Labshake search
Citations for PolyScience :
151 - 200 of 284 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected using PEI (23966-1, Polyscience, Warrington, PA) with the pLentiCRISPRv2-LoxPv1 gRNA containing plasmid ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected using polyethyleneimine MAX (PEI Max) (Polyscience). The amount of the plasmids and PEI Max were optimized in proportion to the relative surface area and the number of cells ...
-
bioRxiv - Biophysics 2022Quote: ... microsphere dispersion (Polybead sulfate, 1.1 µm diameter, Polyscience Inc.) was mixed with rhamnolipid (final concentration of rhamnolipids ...
-
bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
-
bioRxiv - Bioengineering 2022Quote: ... The lentiviral vector was cotransfected into HEK293T cells (RCB2202, RIKEN, Japan) with psPAX2 and pCMV-VSV-G-RSV-Rev using Polyethylenimine Max (Polyscience Inc., Warrington, PA, USA). Culture supernatants containing the virus were collected at 48 hours after transfection ...
-
bioRxiv - Immunology 2022Quote: ... The expression vectors were transiently transfected into HEK-293F cell lines (Clover Biopharma) using PEI (Polyscience) and grown in OPM-293 CD05 medium (OPM ...
-
bioRxiv - Cell Biology 2022Quote: ... Carboxyl-modified fluorescent polystyrene particles (0.50 µm diameter, Polyscience) were introduced hTERT_RPE1 cells by using a ballistic gun (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
-
bioRxiv - Genetics 2022Quote: ... Concanavalin-coated magnetic beads (Polyscience, 86057) were gently washed thrice or four times and resuspended by binding buffer (HEPES-KOH ...
-
bioRxiv - Neuroscience 2022Quote: Cryopreserved aggregates were transferred to a cryomold (PolyScience, #18985) using a 1250μL wide orifice pipette tip and embedded in Tissue-Tek O.C.T ...
-
bioRxiv - Microbiology 2022Quote: Fluoresbrite® Brilliant Blue Carboxylate Microspheres (1.0 µm diameter, Polyscience) were incubated with iC3b (Complement Technology ...
-
bioRxiv - Immunology 2022Quote: ... HEK293-F were transfected with polyethylenimine (Polyscience Europe GmbH) at a ratio of 1:3 DNA:polyethylenimine (Tom et al. ...
-
bioRxiv - Biophysics 2022Quote: ... HFB380-streptavidin coated polystyrene beads (diameter 200 nm; Polyscience) were introduced into the chamber and incubated for 3 min to allow binding with microtubules ...
-
bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl and 0.5 mM spermidine containing protease inhibitor) and captured with 20 μl conacavallin A magnetic beads (Polyscience). Cells were resuspended with antibody buffer (wash buffer with 0.005% digitonin and 2 mM EDTA ...
-
bioRxiv - Biochemistry 2022Quote: ... Temperature was kept at 30 °C using a water bath (PolyScience 7 L, IL). Oxygen consumption reaction mixture was MES 10 mM pH 6 and 500 mg (wet weight ...
-
bioRxiv - Cell Biology 2022Quote: ... using a hypodermic needle into 7.5 μl of Shelton’s Growth Medium (SGM) containing 18.8 μm polystyrene beads (Polybead, Polyscience, Inc. Cat. # 18329). These were mounted between a glass slide and a high precision 1.5H 22 mm x 22 mm glass coverslip ...
-
bioRxiv - Cell Biology 2022Quote: ... 25 mM HEPES pH 7.4) containing 20.8 μm polystyrene beads (Polybead, Polyscience, Inc. Cat. # 18329) between a glass slide and a 1.5 22 mm x 22 mm glass coverslip and sealed with VALAP (1:1:1 vaseline ...
-
bioRxiv - Biophysics 2022Quote: ... the samples were spiked with polystyrene beads (4.5 μm diameter, Polyscience) at a concentration of about 2 × 105 beads per mL as an internal reference ...
-
bioRxiv - Cell Biology 2022Quote: ... using PEI MAX transfection reagent (Polyscience, 24765) for 18 h ...
-
bioRxiv - Genomics 2022Quote: ... Reporter beads were generated by opsonization of dual-colored 1.75 µm latex beads (Fluoresbrite carboxylated 1.75 µm microspheres (yellow-green: 441-nm excitation, 486-nm emission, Polyscience, cat no. 17687-5)) in 50% human male AB serum in PBS for 16 h at 4°C under permanent rotation ...
-
bioRxiv - Microbiology 2022Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
-
bioRxiv - Immunology 2022Quote: ... The recombinant vectors were transiently transfected into HEK293F cells with polyethyleneimine (Polyscience). After three days of expression ...
-
bioRxiv - Neuroscience 2022Quote: ... coverslipped with Aqua Polymount (Polyscience) and subsequently imaged on a Leica SP5-2 confocal microscope.
-
bioRxiv - Microbiology 2022Quote: ... Coverslips were placed on glass slides and samples were preserved with aqua polymount mounting medium (ID#18606, Polyscience).
-
bioRxiv - Pathology 2022Quote: ... Fluoresbrite® YG Microspheres 3.00μm from Polyscience Inc (Warrington ...
-
bioRxiv - Neuroscience 2022Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
-
bioRxiv - Plant Biology 2022Quote: ... 24 h after seeding cells were transfected using a polyethylenimine-based (PEI, linear, MW: 25 kDa, Polyscience) method ...
-
bioRxiv - Neuroscience 2022Quote: ... and an ITR-flanked transgene using polyethylenimine (PEI, Polyscience, 24765-1). AAVs were harvested from the cells and the media 3 days post transfection and purified by ultracentrifugation over iodixanol gradients ...
-
bioRxiv - Neuroscience 2022Quote: ... using Aqua Polymount (Polyscience Inc, 18606-20) and stored at 4°C to dry for at least 16 hours prior to imaging.
-
bioRxiv - Molecular Biology 2022Quote: pCSII-EF-MCS- plasmids expressing Flag-tagged FANCJ WT and AALA mutant were transfected into HEK 293T (shown in Figure 1C and 5A) or FANCJ-KO HeLa cells (shown in Figure 7B) using poly-ethylenimine (PEI, Polyscience, Inc.). At 48 hr post-transfection ...
-
bioRxiv - Immunology 2022Quote: The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... CHRD and human CAP1 expression vectors (250 μg) using polyethylenimine (750 μg; Polyscience). Transfected cells were cultured at 37°C under 6% CO2 on an orbital shaker platform rotating at 135 rpm for 7 days before harvesting ...
-
bioRxiv - Biochemistry 2022Quote: These experiments were carried out using a stopped-flow spectrometer (SX20, Applied Photophysics) equipped with a 7-liter refrigerated circulator (Polyscience) at 22 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... 90 μm Polybead Microspheres (Polyscience, Inc.) were placed between the slide and a No ...
-
bioRxiv - Microbiology 2022Quote: PEI MAX (Polyscience, Inc.) was used to transfect HEK293FT cells with selected plasmids ...
-
bioRxiv - Developmental Biology 2021Quote: ... 10 µl Concanavalin-coated magnetic beads (Polyscience, Cat 86057) for each sample were gently washed twice ...
-
bioRxiv - Molecular Biology 2021Quote: Cell transfections were performed using polyethyleneimine (PEI) reagent (Polyscience Inc., 23966) or jetPRIME (Polyplus transfection ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The cultures were transfected using PEI-25K (Polyscience) with cells grown to a density of 3 million cells per mL and cultivated for 4-5 days ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We equilibrated individuals in a circulating water bath (Polyscience PP15R-30) with Polycool HC -50 anti-freeze liquid ...
-
bioRxiv - Neuroscience 2021Quote: Transient transfections were performed for 24 h using the linear polyethylenimine (PEI, Polyscience, #24765) method [52] with a final concentration of 2 µg/ml PEI and 6 μg of DNA for a 10 cm diameter dish with 10 ml of medium.
-
bioRxiv - Molecular Biology 2021Quote: ... The cultures were transfected using PEI-MAX (Polyscience) with cells grown to a density of 3.0 million cells per mL and cultivated for 3 days ...
-
bioRxiv - Molecular Biology 2021Quote: ... Antibodies were expressed by transient co-transfection of both heavy and light chain plasmids in Expi293F cells using PEI MAX (Polyscience) transfection reagent ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 cells were transiently transfected by endotoxin free construct of MHV-Fc virolectin or its mutant in presence of polyethyleneimine (PEI, Polyscience Inc, 23966). Precisely ...
-
bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
-
bioRxiv - Immunology 2021Quote: ... PEI with an average molecular mass of 25 kDa (PEI25K) (Polyscience, Warrington, PA, United States) was gently vortexed ...
-
bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
-
bioRxiv - Biophysics 2021Quote: The precision of the positional measurements was verified using 300-nm latex beads (PolyScience, Niles, IL, USA) that were similar in size to the cargos ...
-
bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2021Quote: ... Transfection was conducted with 4 μl of PEI (Polyscience) following the manufacturer’s manual and 1μg of CR plasmids and 1μg of rgRNA plasmids ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfection was performed using polyethylenimine Max (Polyscience) according to the manufacturer’s instructions.