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Citations for Lucigen :
401 - 450 of 719 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and expressed in BL-21 (DE3) cells (Lucigen, Middleton, WI). Bacterial cultures were grown overnight at 37°C in 2x YT (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporations were recovered in 1 mL recovery media (Lucigen) for 1 hour and subsequently grown overnight in LB + selection.
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μL of Endura electrocompetent bacteria (Lucigen 60242-1) were transformed with 150 ng of library DNA using a 0.1 cm Gene Pulser electroporation cuvette (Bio-Rad 1652083 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then thawed and 35,000 U of Ready-Lyse (Epicentre) was added ...
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bioRxiv - Genomics 2020Quote: ... They were then amplified using electroporation in Endura electrocompetent cells (Lucigen), to achieve at least 250 colonies per sgRNA in the library ...
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bioRxiv - Genomics 2020Quote: ... using a Gibson assembly master mix (New England Biolabs).Gibson assembly products were transformed into electrocompetent cells (E. cloni, Lucigen) and plated on 245mm x 245mm square LB-agar plates to obtain the sufficient number of bacterial colonies at a ∼50× library coverage ...
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bioRxiv - Bioengineering 2019Quote: ... genomic DNA was extracted from cells using Quick Extract (Lucigen, QE09050) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... coli Cloni® 10G cells (Lucigen, USA; 1ul crude reaction mix). Kanamycin selected clones were screened by colony PCR and verified by sequencing ...
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bioRxiv - Genetics 2020Quote: ... DNA collection from cultured cells was performed using QuickExtract (Lucigen QE09050).
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bioRxiv - Microbiology 2020Quote: ... NEB Builder reaction mixtures were transformed into E.coli EPI300 cells (Lucigen) and successful integration of the S protein cDNA was confirmed by Sanger sequencing ...
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bioRxiv - Neuroscience 2021Quote: NeuroD1-pNOTAT was transformed into C41 competent cells (Lucigen, Middleton, WI) and induced with IPTG at 0.1mM for protein expression ...
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bioRxiv - Microbiology 2021Quote: ... and transformed into the BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen). The replicon vector was maxipreped using a NucleoBond Xtra BAC kit (Takara Bio.).
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bioRxiv - Genomics 2022Quote: ... The product was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli TransforMax Epi300 electrocompetent cells (Ref. EC300110, Epicentre, Madison, WI) and Saccharomyces cerevisiae VL6-48N strain [30] were used to propagate the pCC1BAC-His3 containing viral cDNA ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were lysed with 4000U of RNase-free lysozyme (Lucigen) at 37 °C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells (Strand et al., 2014; Lucigen, Middleton, WI, Catalog #EC300110) were transformed via heat shock with sequence-verified pPtPBR1 episomes ...
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bioRxiv - Physiology 2022Quote: ... sorted (eGFP+) cells was extracted using QuickExtract DNA Extraction Solution (Lucigen). EnGen Mutation Detection Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: Ligation products were transformed into Lucigen Endura Electrocompetent Cells (Lucigen, 60242) using a Gene Pulser II (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in a Ready-Lyse lysozyme solution (Epicentre Technologies) according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... The construct was transformed into chemically competent C41 (DE3) cells (Lucigen) and plated after heat shock and recovery on LB agar supplemented with ampicillin ...
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bioRxiv - Genetics 2023Quote: ... Cell pellets were resuspended in 50 µL of Quick Extract (Lucigen), and genomic DNA was prepared per the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen) using Gene Pulser Xcell Electroporation Systems (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligated pUSEPR plasmid DNA was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37⁰C ...
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bioRxiv - Microbiology 2024Quote: ... the phagemids were electroporated into phage-competent TG1 bacterial cells (Lucigen) with the MicroPulsuerTM system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... in combination with 1 µl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... in combination with 1 μl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Immediately after electroporation 1 ml of prewarmed recovery medium (Lucigen, #800261 was added to the bacteria and the cells were incubated for 1 hour at 37 °C shaking at 250 rpm in a bacterial incubator ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Biochemistry 2021Quote: ... Cloning was performed by Gibson assembly (46) using E.cloni 10G cells (Lucigen) and point mutations were performed using the Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: DNA was extracted from cells using the QuickExtract DNA Extraction Solution (Lucigen) and sequencing was performed with 110x coverage using 100 base pair paired end read lengths ...
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bioRxiv - Immunology 2022Quote: ... The Gibson assembly reaction was electroporated using Endura(tm) ElectroCompetent Cells (Lucigen), plated on LB + Ampicillin plates at an estimated 150,000 colonies per replicate library to limit library complexity ...
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bioRxiv - Genomics 2019Quote: gDNA was extracted by cell lysis using QuickExtract DNA Extraction Solution (Lucigen). From a confluent culture in 96-well plate ...
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bioRxiv - Genomics 2019Quote: RNA from 100,000 K562 cells was extracted with RNA QuickExtract (Lucigen QER090150). RNA was treated with DNaseI from the same kit ...
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bioRxiv - Developmental Biology 2021Quote: ... approximately 30,000 cells were lysed in 25ul Quick Extract Buffer (Lucigen #QE09050), according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... BAC plasmid was delivered into BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen) by electroporation and bacteria was propagated according to the manufacturer’s guide ...
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bioRxiv - Genomics 2021Quote: ... and 5 electroporations were performed into Endura electrocompetent cells (Lucigen, Middleton WI) and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Microbiology 2022Quote: ... The library was electroporated into Endura electrocompetent cells (# 60242; Lucigen Bioresearch Technologies) and plasmid was prepared from > 80 million bacterial colonies (>1,000X representation) ...
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bioRxiv - Immunology 2023Quote: 0.1 × 106 cells were collected and lysed in QuickExtract™ (Lucigen Epicentre) according to the supplier’s instructions ...
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bioRxiv - Immunology 2023Quote: 0.1 × 106 cells were collected and lysed in QuickExtract™ (Lucigen Epicentre) according to the supplier’s instructions ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... The resulting ligated DNA was transformed into electrocompetent cells E.coli SS320 (Lucigen) and propagated overnight.
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was harvested from cell pellets using QuickExtract DNA Extraction Solution (Lucigen). The ROS1 kinase and C-terminal domains were PCR amplified using the primers ROS1 5707F (5’-GACAAAGAGTTGGCTGAGCTG-3’ ...
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...