Labshake search
Citations for Lucigen :
201 - 250 of 719 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
2> Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Microbiology 2019Quote: ... were used to amplify lnt that had been disrupted by the insertion of Tn5
2> (Epicentre) [6] ... -
bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
2> Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Neuroscience 2021Quote: ... We used 1.5 μL of the ligation mix and electroporated 30 μL competent bacteria (Lucigen, 60242-2) with ...
-
bioRxiv - Synthetic Biology 2022Quote: ... DNA of 2 mL overnight culture was extracted with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and whole PCR Tag analysis was performed on population level to test the general presence of all tRNAs within the population (data not shown) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G SOLO cells (Lucigen). Whole transformations were plated on LB-agar + 25 μg/mL chloramphenicol and grown overnight ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G cells (Lucigen, USA). Cells were grown overnight at 37 °C on LB-agar + 50 μg/mL kanamycin plates and transformants scraped for plasmid DNA isolation ...
-
bioRxiv - Microbiology 2019Quote: ... coli 10G ELITE cells (Lucigen) via electroporation ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... cloni 10G electrocompetent cells (Lucigen) using the accompanying protocol ...
-
bioRxiv - Microbiology 2021Quote: ... coli C41(DE3) cells (Lucigen). Transformants were grown at 37°C and 225 rpm until an OD600 of 0.4–0.6 was reached ...
-
bioRxiv - Biochemistry 2021Quote: ... coli C43(DE3) cells (Lucigen). Transformed cells were recovered for 45 minutes at 37 °C then and diluted into 200 mL of sterile LB media with the added carbenicillin ...
-
bioRxiv - Biochemistry 2020Quote: ... Cloni 10G ELITE cells (Lucigen) in 1.0-mm Bio-Rad cuvettes using a Gene Pulser Xcell Electroporation System (settings ...
-
bioRxiv - Microbiology 2021Quote: ... coli EPI300 cells (TransforMaxTM, Lucigen) and electroporation for transformation highly increased cloning efficiency for the CRISPR-Cas12a constructs in E ...
-
bioRxiv - Microbiology 2020Quote: ... cloni Supreme electrocompetent cells (Lucigen), providing > 10,000x coverage of both libraries in total transformants.
-
bioRxiv - Microbiology 2022Quote: ... coli cells (Lucigen, Middleton, WI) electroporated with Rmona658B genomic DNA that had been linearized by digestion with SmaI and ligated into the linear vector pJAZZ (Lucigen) ...
-
bioRxiv - Genetics 2022Quote: ... Endura Electrocompetent cells from Lucigen; Mighty Mix T4 DNA ligase from Takara ...
-
bioRxiv - Biochemistry 2022Quote: ... Cloni 10G electrocompentent cells (Lucigen) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... cloni 10G competent cells (Lucigen). Protein expression was performed as described (45) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... coli cells (Lucigen, Cat #: EC300110) were thawed on ice for 20 mins ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli BL21(DE3) cells (Lucigen).
-
bioRxiv - Molecular Biology 2023Quote: ... coli C43 (DE3) cells (Lucigen) while VLCADWT (res ...
-
bioRxiv - Immunology 2023Quote: ... coli ER2738 cells (Lucigen Corporation) for titer determination and overnight amplification ...
-
bioRxiv - Cancer Biology 2023Quote: ... coli Endura electrocompetent cells (Lucigen), plated onto prewarmed LB medium/agar containing 100 μg/mL carbenicillin in a 245 mm square bioassay dish (Corning 431111) ...
-
bioRxiv - Immunology 2022Quote: ... from Lucigen (60810-1), were used in this study ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
-
bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
-
bioRxiv - Microbiology 2021Quote: ... Transposon mutagenesis was performed with the EZ-Tn5
2>Tnp Transposome kit (Epicentre, Illumina, CA, USA) and the insertion site of all mutants was determined via arbitrary PCR (Segev et al ... -
bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
-
bioRxiv - Cell Biology 2022Quote: ... coli 10G SUPREME electrocompetent cells (Lucigen). This plasmid pool was used to produce lentivirus-containing supernatants in HEK293T cells ...
-
bioRxiv - Biochemistry 2020Quote: ... cloni® 10G Electrocompetent Cells (Lucigen) (> 5 x109 cfu/μg ...
-
bioRxiv - Cell Biology 2020Quote: ... coli 10G chemically competent cells (Lucigen). Plasmids were isolated from transformants and Sanger sequencing was used to identify those containing the correct insertion.
-
bioRxiv - Synthetic Biology 2022Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen) were transformed with each library and expressed in a 96-well deep well format.21 General methods for liquid handling and fluorescence plate reader screening at pH 5 ...
-
bioRxiv - Biophysics 2020Quote: ... coli OverExpress C43 (DE3) cells (Lucigen) cells were transformed with the pET-26b vector containing the DNA of GLIC mutants ...
-
bioRxiv - Immunology 2020Quote: ... Escherichia coli C43(DE3) cells (Lucigen) transformed with pOPTH-1124 were grown in terrific broth medium ...
-
bioRxiv - Genomics 2021Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen) (2 μl of the ligation mix per reaction ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen E. cloni 10G); post-heat shock recovery time was limited to 15-20 minutes to avoid cell division during recovery and ensure that each resulting colony was the result of an independent transformation event ...
-
bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen E. cloni 10G). Around 800 thousand colonies were recovered ...
-
bioRxiv - Immunology 2022Quote: ... transformed into Endura electrocompetent cells (Lucigen) and maxiprepped (Plasmid Plus Midi or Maxi Kit ...
-
bioRxiv - Immunology 2022Quote: ... transformed into Endura electrocompetent cells (Lucigen) and midi- or maxiprepped (Plasmid Plus Midi or Maxi Kit ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen) were transformed with 2.5 µl of the eluate each ...
-
bioRxiv - Bioengineering 2023Quote: ... coli 10G Chemically Competent Cells (Lucigen) were transformed with the ligation mixtures and plated on LB plates containing 50 µg kanamycin (LB-Kan ...
-
bioRxiv - Biochemistry 2023Quote: ... coli ER2738 cells (Lucigen, Middleton, WI) were transformed with the resulting phagemids by electroporation followed by single-colony PCR and DNA sequencing to assess correct size and sequence of the inserts ...
-
bioRxiv - Biochemistry 2023Quote: ... cloni 10F ELITE Electrocompetent cells (Lucigen), and plated on two 140 mm Petri dishes containing LBkan-agar ...
-
bioRxiv - Microbiology 2024Quote: ... cloni 10G Elite competent cells (Lucigen) via electroporation ...
-
bioRxiv - Biochemistry 2023Quote: ... cloni 10F ELITE Electrocompetent cells (Lucigen), and plated on two 140 mm Petri dishes containing LBkan-agar ...
-
bioRxiv - Microbiology 2019Quote: ... Isolated RNA was treated with 1 μL (1 unit) of Baseline Zero DNase (Epicentre) at 37°C for 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... The puck was immersed in 200 µL of hybridization buffer (6X SSC, 2 unit / µL Lucigen NxGen RNAse inhibitor) for 30 minutes at 37°C for the binding of RNA to the oligos ...
-
bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.