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Citations for Lucigen :
301 - 350 of 719 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 2 μl Maxima H- RTase (EP0751 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen) and 31 μl water ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 U/ml Rnase Inhibitor (Lucigen), 2.5 μM Template Switch Oligo ...
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bioRxiv - Genomics 2021Quote: ... 6uL 100mM MgSO4 (Lucigen, F98695-1), 10uL Betaine (Lucigen ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Systems Biology 2023Quote: ... cloni 10G supreme (#60081-1; Lucigen).
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bioRxiv - Systems Biology 2023Quote: ... cloni 10G supreme (#60081-1; Lucigen). To estimate the approximate complexity of the resulting libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
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bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
2> Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ... -
bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (E. cloni 10G Elite, Lucigen, USA). Further details on the microfluidic screening can be found in the Supplementary Information.
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bioRxiv - Cell Biology 2019Quote: ... then transformed into Endura ElectroCompetent cells (Lucigen, 60242) according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2019Quote: ... coli 10G chemically competent cells (Lucigen, Middleton, WI). Colonies resistant to kanamycin (Kan ...
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bioRxiv - Cell Biology 2021Quote: ... coli EXPRESS BL21(DE3) chemically competent cells (Lucigen). A dense overnight culture was diluted 1:100 into 15 mL LB (Miller ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were confirmed by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Biophysics 2019Quote: ... cloni 10G chemically competent cells (Lucigen, Middleton, WI). Colonies resistant to kanamycin (Kan ...
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bioRxiv - Genetics 2019Quote: ... and transformed into electrocompetent cells (Lucigen, Middleton, WI) via a MicroPulser (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... was transformed into electrocompetent Endura cells (#60242, Lucigen) in quadruplicates ...
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested using Quick Extract solution (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... coli SS320 host cells (Lucigen Corporation, Middleton, WI) were electroporated using 30-50 ng of each sub-library DNA in a 0.1 cm cuvette with the following settings ...
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bioRxiv - Genomics 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were lysed by QuickExtraction solution (Lucigen) per the manufacturer’s instruction at 65°C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were verified by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
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bioRxiv - Developmental Biology 2023Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
2> Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Developmental Biology 2024Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Neuroscience 2019Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA collected from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 u/µl NxGen RNase inhibitor (Lucigen) and 5% (v/v ...
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bioRxiv - Bioengineering 2019Quote: ... and 1 μL 10X ampligase buffer (Lucigen). Circularization was achieved under the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl of RiboGuard RNase Inhibitor (Epicentre) was added to the eluted RNA to protect against ribonucleases ...
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bioRxiv - Genomics 2019Quote: ... 1 μL ScriptSeq Index PCR Primer (Epicentre), 1 μL Reverse PCR Primer (Epicentre) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:200 diluted endonuclease (Lucigen: OC7850K) was immediately added to the pulverized tissue at a constant ratio of 15μL/mg of tissue ...
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bioRxiv - Genomics 2021Quote: ... 10uL 10x OmniAmp buffer (Lucigen, F883707-1), 8uL dNTP (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1 U/μL Rnase Inhibitor (Lucigen, #F83923), 2.5 μM Template-Switching Oligo primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 U/mL Baseline-Zero DNase (Lucigen), and 0.5 mM PMSF ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x Ampligase® Reaction Buffer (Lucigen), 10 U Ampligase (Lucigen) ...
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bioRxiv - Genomics 2022Quote: ... 1 U/μL RNase Inhibitor (NxGen, Lucigen), 1 U/μL reverse transcriptase (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... in 1× RNase R buffer (#RNR07250; Epicentre) in a 10 µl reaction volume at 37 °C for 10 min followed by heat inactivation at 95 °C for 3 min ...