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Citations for Lucigen :
351 - 400 of 719 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... pNT-15 was constructed using NEB HiFi DNA Assembly with a gBlock® from IDT (Coralville, IA) and cloning vector pSMART-HCKan (Lucigen, #40704-2). All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Neuroscience 2020Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, Message BOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... TOPO plasmids were electroporated into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... The purified product was transformed into electrocompetent cells (Lucigen) via a MicroPulser (Bio-Rad) ...
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bioRxiv - Biophysics 2020Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen Corporation, Middleton, USA) were transformed with a pT7 plasmid containing the ClyA-AS gene ...
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bioRxiv - Biophysics 2020Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) followed by 0.5% SDS treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly resuspended in 50 µl QuickExtract (Lucigen) and incubated at 65 °C for 20 min and 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) in TE for 60 min ...
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bioRxiv - Bioengineering 2023Quote: ... TG1 electrocompetent cells were procured from Lucigen (Middleton, WI). M13KO7 helper phage particle ...
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bioRxiv - Genomics 2023Quote: ... then were transformed into Endura Electrocompetent Cells (Lucigen, 602422) by electroporation (Gene Pulser Xcell Electroporation System ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was resuspended in Quick-Extract (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The final product was transformed into electrocompetent cells (Lucigen), incubated overnight in LB broth at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The electroporated cells were recovered in recovery medium (Lucigen) for 1 h and then plated on Terrific Broth (TB ...
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bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
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bioRxiv - Microbiology 2020Quote: Samples were analyzed directly or mixed 1:1 with one of the following buffers: Quick Extract DNA Extraction Solution (Lucigen), Virotype Tissue Lysis Reagent (INDICAL BIOSCIENCE GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology) ...
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bioRxiv - Genomics 2020Quote: ... 1 ul Hybridase Thermostable RNase H (Lucigen, H39500) and 1 ul 10 X digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... electroporated in triplicate into Endura electrocompetent cells (Lucigen, Middleton WI), and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Microbiology 2021Quote: ... The vector was transformed into BigEasy-TSA Electrocompetent Cells (Lucigen) for propagation ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet resuspended in 100μL QuickExtract solution (Lucigen #QE09050), transferred to PCR-tubes and incubated in a thermocycler for 15 minutes at 68°C ...
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bioRxiv - Bioengineering 2021Quote: ... Each library was independently electroporated into Endura electrocompetent cells (Lucigen), plated on agar bioassay plates ...
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bioRxiv - Genetics 2019Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was extracted from HEP3B cells using QuickExtract (Epicentre).
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bioRxiv - Genomics 2022Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Genomics 2022Quote: ... Coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Genetics 2023Quote: ... The purified product was electroporated into Endura competent cells (Lucigen). We extracted the plasmid pool using the Plasmid Maxi prep kit ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were lysed using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Genomics 2023Quote: ... coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Systems Biology 2022Quote: ... gDNA was isolated from obtained cell clones using QuickExtract (Lucigen), the gRNA-targeted sites were PCR-amplified ...
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bioRxiv - Genetics 2023Quote: ... 5 Gibson reactions were electroporated into supercompetent cells (Endura, Lucigen) and grown in liquid culture to give a coverage of around 100 million barcodes ...
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... cells were lysed by QuickExtract™ DNA Extraction Solution (Lucigen) to extract genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... then electroporated 10 uL into Endura competent cells (Lucigen #602422) and then expanded the cells in liquid culture for 18 hours at 30°C and purified library plasmid with the Nucleobond Xtra Midi EF kit (Machery-Nagel #740420.50) ...