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Citations for Lucigen :
251 - 300 of 420 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans Fosmid Library (Source BioScience) and were amplified in bacteria using CopyControl Induction Solution (Lucigen, #CCIS125) and purified using a DNA midi prep kit (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) to the cells ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre, Cat # QE09050) to the cells ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Bioengineering 2024Quote: ... DNA extraction from various hiPSC clones was carried out using the QuickExtract DNA Extract Solution (Epicentre), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from each CFU recovered using the QuickExtract DNA extraction solution (Epicentre, # QE09050) and each colony was screened for the presence of tbpA and lbpA genes ...
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bioRxiv - Molecular Biology 2024Quote: ... a fraction of the cells was used for genomic DNA extraction using QuickExtract DNA Solution (Lucigen). PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted from individual samples using 40 µL QuickExtract DNA extraction solution (Lucigen QE0905T). Embryos were incubated for 15 min at 25 °C ...
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bioRxiv - Genetics 2024Quote: ... they were further expanded and gDNA was harvested using Quick Extract DNA Extract Solution (Lucigen, QE9050). Excision and inversion clones were genotyped via ddPCR and Sanger sequencing (Table S66).
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bioRxiv - Genomics 2020Quote: To test the Terminator™ 5’-phosphate-dependent exonuclease (Lucigen, Cat. # TER5120), 100 ng of total RNA in 2 μL was combined with 18 μL TEX mix ...
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bioRxiv - Genomics 2021Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by CIP (NEB ...
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bioRxiv - Genetics 2021Quote: ... RNA samples for small RNA-seq were treated with 5’polyphosphatase (Lucigen) and prepared using the SMARTer Apollo system with modifications for small RNA library preparation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sample was treated with 20 units of 5’ RNA polyphosphatase (Lucigen) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by quickCIP (NEB ...
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bioRxiv - Immunology 2021Quote: ... Monophosphorylated RNAs were selectively degraded by Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen) and RNAs were 5’dephosporylation by quickCIP (NEB) ...
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bioRxiv - Genomics 2021Quote: ... and 5 electroporations were performed into Endura electrocompetent cells (Lucigen, Middleton WI) and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Genomics 2021Quote: ... Prior to incubation with Terminator™ 5′-Phosphate-Dependent Exonuclease (TEX) (Lucigen) to remove all residual RNAs containing 5’ monophosphate ...
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bioRxiv - Microbiology 2023Quote: ... unligated 5’-P RNA fragments were removed using terminator exonuclease (TEX, Lucigen), followed by ligation of transcriptional start site (TSS)-specific adaptors.
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bioRxiv - Genomics 2023Quote: ... The RNA 5’ ends were dephosphorylated using Heat Labile Alkaline phosphatase (Epicentre), purified using ZYMO columns and treated with RNA 5’ Pyrophosphohydrolase (RppH ...
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bioRxiv - Genomics 2023Quote: ... The eluted RNA was then treated with 5’ Terminator exonuclease enzyme (Epicentre) to remove the uncapped RNA ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA templates were prepared by lysing cells in QuickExtract DNA extraction solution (Lucigen, Cat No. QE0905T). Target regions were amplified by using specific PCR primers (ETV2_F ...
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bioRxiv - Cell Biology 2022Quote: ... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from individual female adult flies from stocks of interest using QuickExtract solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: One swab each was stored in 350 μl Tissue and Cell lysis solution (Lucigen, #MTC096H, Middleton, WI) lysis buffer with 100 μl glass beads (0.1 diameter ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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bioRxiv - Cell Biology 2022Quote: ... the individual clones were expanded and the DNA was isolated using the QuickExtract DNA Extraction Solution (Lucigen). A successful homozygous genomic modification was confirmed by PCR (data not shown ...
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from these clones using the QuickExtract DNA extraction solution (Catalog No. QE09050, Lucigen) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... gDNA isolation was performed from a portion of the clones via QuickExtract DNA Extraction Solution 1.0 (Epicentre). Genotyping primers were created by designing flanks around the mutation site ...
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bioRxiv - Molecular Biology 2024Quote: ... for sorting and then genomic DNA was extracted using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre), adhering to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... an aliquot of cells was used to extract the DNA by QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). To assess the genomic editing efficiency ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... the genomic DNA derived from transfected single cell colonies was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for Sanger sequencing and the genomic DNA with biallelic editing was further subjected to whole-genome sequencing ...
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bioRxiv - Systems Biology 2021Quote: ... We then added 3.8 mL of 1x BMg/THS buffer and 0.8 μL of ReadyLyse lysozyme solution (Lucigen), 40 μL of 10 mg/mL RNase A ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from 10,000 – 50,000 target cells by resuspending them in a QuickExtract DNA Extraction Solution (LGN-QE09050, Lucigen). PCR amplification of the targeted junction from the wild-type and tagged cells was performed using primers designed to anneal outside of the homology arms of the specific gene ...
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bioRxiv - Biochemistry 2021Quote: 5’ RNA polyphosphatase (Rpp) and T4 Polynucleotide Kinase (PNK) were purchased from Epicentre and NEB ...