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Citations for Lucigen :
1 - 50 of 420 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... coli 5-alpha Chemically Competent cells (Lucigen), performing a thermal shock for 45 seconds at 42°C followed by 2 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Cell Biology 2024Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Synthetic Biology 2022Quote: ... with QS solution (1.5 M NaCl, 100 mM MOPS, 15% v/v isopropanol) and PlasmidSafe (Epicentre) were used in lieu of the Qiagen Large Construct Kit and Qiagen exonuclease ...
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bioRxiv - Genomics 2024Quote: ... 10% v/v Phi29 enzyme (Lucigen), 0.2 mg/ml BSA ...
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bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
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bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
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bioRxiv - Microbiology 2020Quote: ... The samples were chilled on ice for 5 minutes and 175 μl of MPC protein precipitation solution (Lucigen, USA) was added to 300 μl of the lysed sample and vortexed vigorously for 10 seconds ...
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bioRxiv - Microbiology 2024Quote: ... v.2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions and quantified with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was spun down at 5,000 x g for 5 minutes and the pellet was resuspended in 100 µl of QuickExtract™ DNA Extraction Solution (Lucigen) and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre ...
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bioRxiv - Neuroscience 2021Quote: ... adding CopyControl solution (EpiCentre) 2 hrs before the miniprep ...
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bioRxiv - Microbiology 2021Quote: ... 5’PPP structures were then converted into 5’P ends using RNA 5’ Polyphosphatase (5’PP, Epicentre), to which RNA adapters were ligated ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: Quick extract DNA extraction solution (Lucigen) was tested in accordance with the manufacturer’s suggested buffer to sample ratio ...
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bioRxiv - Genetics 2021Quote: ... 25 μM ATP standard solution (Epicentre) was used for preparing a concentration gradient (0 ...
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bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Immunology 2024Quote: ... QuickExtract DNA Extraction Solution 1.0 (Epicentre) was used to generate bulk gDNA from dissociated cells to validate editing efficiency before clonal selection ...
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bioRxiv - Microbiology 2020Quote: ... 5′-deadenylase (Epicentre) was used to deadenylate the pre-adenylated linkers ...
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bioRxiv - Microbiology 2024Quote: ... the 5’PPP structures were removed using RNA 5’ polyphosphatase (Epicentre). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL DNA QuickExtract solution (Lucigen QE09050) was added ...
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bioRxiv - Microbiology 2022Quote: ... 2 ml/L fosmid autoinduction solution (Epicentre), each plate supplemented with 0.3% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μl QuickExtract DNA Extraction Solution (Lucigen) was used following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... or QuickExtract DNA extraction solution (Lucigen, #QE0905T) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... or QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). A 601 bp genomic region (chr11:92,975,259- 92,975,860 ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were polyA-tailed and 5’-PPP-ends were trimmed to 5’-P-ends via RNA 5’-polyphosphatase (Epicentre). First-strand cDNA synthesis was performed using an oligo(dT)-adapter and M-MLV reverse transcriptase followed by high-fidelity PCR amplification of the cDNA using primers suitable for Illumina TruSeq sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg RNA was incubated with 5 U RNase R (#RNR07250; Epicentre) in 1× RNase R buffer (#RNR07250 ...
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bioRxiv - Microbiology 2023Quote: ... Triphosphate in 5’ were removed using a RNA 5’ polyphosphatase (Euromedex Lucigen) and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman) ...
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bioRxiv - Biochemistry 2024Quote: ... and 5’ polyphosphatase (Lucigen), using 1 μL of enzyme per 5 μg of uncapped RNA ...
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bioRxiv - Biochemistry 2024Quote: ... 5’ polyphosphatase buffer (Lucigen) and 5’ polyphosphatase (Lucigen) ...
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bioRxiv - Genetics 2024Quote: ... and aliquots mock-treated or treated with T4-endonuclease V (T4endoV, Epicentre). DNA was electrophoresed in 1.3% alkaline agarose gels ...
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bioRxiv - Microbiology 2023Quote: ... Successful RppH treatment was verified by incubating 100 ng of 5’P or 5’PPP transcripts with 20 U RiboLock and 1 U Terminator™ 5’Phosphate-Dependent Exonuclease (Lucigen) in 1x TEX Buffer B for 30 min at 42°C ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was extracted (DNA extraction solution, Epicentre Biotechnologies) and edited regions were specifically amplified by PCR (primers are listed in Supplementary Table 1) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Microbiology 2021Quote: ... resuspended a 0.5 mL ReadyLyse lysozyme solution (Epicentre), and then sample incubated for one hour at 37°C with shaking ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... and lysed with Ready-lyse lysozyme solution (Epicentre). Lysates were sonicated using a sonicator (QSonic ...
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bioRxiv - Neuroscience 2020Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen) followed by PCR and Sequencing to determine efficient rescue.
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bioRxiv - Microbiology 2021Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Epicentre), 2 U/mL Zymolyase (Zymo research) ...
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested using Quick Extract solution (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were lysed by QuickExtraction solution (Lucigen) per the manufacturer’s instruction at 65°C for 15 min ...
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bioRxiv - Immunology 2024Quote: Genomic DNA was isolated using QuickExtract solution (Lucigen). Reactions were incubated for 10 min at 65 °C followed by 5 min at 98 °C and cooling to 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient repair of the mutation.
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bioRxiv - Microbiology 2022Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Lucigen), 2 U/mL Zymolyase (Zymo Research Corporation) ...
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bioRxiv - Microbiology 2022Quote: ... and 10,000 units of ReadyLyse Lysozyme solution (Lucigen). Samples were subjected to bead beating for ten minutes at maximum speed on a vortex mixer with 0.5 mm glass beads (MoBio) ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...