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Citations for Lucigen :
151 - 200 of 420 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cloni 5-α (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Genomics 2020Quote: ... media was aspirated and 50-100 μL QuickExtract™ DNA Extraction Solution (Lucigen) were added to each well ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted 48 hrs after electroporation using QuickExtract DNA Extraction Solution (Epicentre). Genomic DNA was extracted in the same way from NK cells for off-target studies comparing WT and HiFi Alt-R Cas9 RNP complexes with editing mediated by optimized delivery conditions ...
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bioRxiv - Microbiology 2020Quote: Saliva samples were treated with the Quick ExtractTM DNA Extraction Solution (QE, Lucigen) by mixing 50 μl of saliva with 50 μl of the QE reagent and heating for 5 minutes at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were expanded and genomic DNA extracted (QuickExtract DNA Extraction Solution; Epicentre, #QE09050), as described above ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI ...
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bioRxiv - Biophysics 2022Quote: ... DNA from these cells was extracted using QuickExtract DNA extraction solution (Lucigen # QE09050) and used as a template for confirmation of homologous recombination by PCR and Sanger sequencing.
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bioRxiv - Bioengineering 2022Quote: DNA was extracted from harvested cells using the QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... Genome DNA was extracted from survival cells using QuickExtract DNA Solution 1.0 (Epicentre), and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were re-suspended in 20 μl of DNA Extraction Solution (Lucigen), and incubated at 68°C for 6 min ...
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bioRxiv - Developmental Biology 2020Quote: ... and Sanger sequencing was then extracted using QuickExtract™ DNA Extraction Solution (Lucigen). The PDX1 knockout line was generated in an analogous way.
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bioRxiv - Molecular Biology 2020Quote: Harvested cells were lysed using QuickExtract DNA Extraction Solution (Lucigen, Cat. No. QE09050) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the genomic DNA of each clone was extracted by using QuickExtract solution (Epicentre) and PCR was carried out ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was extracted with QuickExtract DNA Extraction Solution (catalog no. QE09050; Lucigen, Biosearch technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA of cells was extracted using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2021Quote: ... The gRNA-msDNA hybrid was extracted with the QuickExtract RNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA from each cell line was isolated (QuickExtract DNA Extraction Solution, Lucigen). Total RNA was isolated from each cell line (Maxwell RSC simplyRNA kit ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from the cells using QuickExtract DNA Extract Solution (Lucigen, QE9050). Clones were genotyped by PCR and Sanger Sequencing (Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from cell pellets with QuickExtract DNA Extraction Solution (Lucigen) via manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from individual colonies was isolated using QuickExtract DNA extraction solution (Lucigen). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from clones using QuickExtractTM DNA isolation solution (Lucigen #QE09050). PCRs were performed with KOD hot-start polymerase (Merck #71086) ...
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bioRxiv - Genomics 2024Quote: ... The gapfill and ligation solution was composed of 1X Ampligase buffer (Lucigen A3210K), 50 nM dNTPs (New England Biolabs N0447L) ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Molecular Biology 2023Quote: DNA from GFP-positive cells was isolated using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Neuroscience 2023Quote: ... the genomic DNA was extracted from single clones using QuickExtract solution (Lucigen, QE090500) and HDR was verified by locus specific PCR using DreamTaq DNA Polymerase (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... extraction of genomic DNA was performed with QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2024Quote: ... DNA was extracted using Quick Extract DNA extraction solution (Epicentre Catalog no. QE09050). Colonies were screened by PCR and sequenced using primers flanking each SNP (Primer sequences are provided in Table S6A) ...
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bioRxiv - Genetics 2024Quote: ... The cell pellet was resuspended in QuickExtract DNA Extraction Solution (Lucigen, United Kingdom) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA of sorted cells was extracted with QuickExtractTM DNA Extraction Solution (Lucigen) and amplified by PCR using Phusion High-Fidelity DNA Polymerase (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was extracted from hPSCs using Quick Extract DNA solution (Lucigen, QE09050). Gene targeting specific amplicon was amplified using one primer on the bGH poly A sequence of the insert and the other primer outside the right homology arm ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was isolated using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Neuroscience 2020Quote: ... using Inpp5e WT forward primer 5’-GAGAAGCTGATAGATGGCTAGG and Inpp5e WT reverse primer 5’-AACCAGAAGACCTCATCAAACC and EconoTaq PCR according to manufactures specifications (Lucigen corporation, WI) (Fig ...
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bioRxiv - Microbiology 2021Quote: ... 3-5 μl Hybridase™ Thermostable RNase H (Lucigen) and 7 μl 10x RNase H buffer preheated to 45°C was added ...
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bioRxiv - Bioengineering 2024Quote: ... 5 ’phosphorylated DNA ogligomer was circularized using Circligase (Lucigen) under the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... samples were further treated with RNA 5′- polyphosphatase (Epicentre) as previously described[26].
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bioRxiv - Developmental Biology 2021Quote: ... ear clips were lysed by incubation in 25 μl of QuickExtract Solution (QE09050, Lucigen) at 65 °C and shaking at 600 rpm for 30 min ...
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bioRxiv - Genomics 2020Quote: The genomic DNA of collected cells was extracted using QuickExtract DNA Extraction Solution (Epicentre). Genomic PCR reaction was performed using 100 ng genomic DNA ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Biochemistry 2021Quote: ... from which the genomic DNA was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for subsequent sequencing analysis.
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA of expanded clones was isolated using QuickExtract DNA extraction solution (Lucigen, QE0905T). PCR was performed by amplifying a 241bp area surrounding the mutation site ...
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bioRxiv - Neuroscience 2021Quote: ... the genomic DNA was extracted using the QuickExtract DNA Extraction solution (Epicentre, Madison, USA), according to the manufactureŕs instructions ...