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Citations for Lucigen :
551 - 600 of 760 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Microbiology 2023Quote: ... Transposomes were prepared using 100 ng transposon DNA and EZ-Tn5 transposase (Lucigen, USA) according to the suppliers specifications ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Microbiology 2024Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sing1 gDNA was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen, MPY80200). Total RNA was extracted with TRIzol™ (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was precipitated using ispopropanol and contaminating RNA removed using RiboShredder™ RNase Blend (Epicentre). RNase was removed using an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was harvested using QuickExtract solution (screening of CRISPR/Cas9 clones; Epicentre EPI-QE0905T) or NucleoSpin Tissue kit (routine genotyping ...
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bioRxiv - Systems Biology 2021Quote: ... thermophilus HB8 was isolated using the MasterPure Complete DNA purification Kit (Lucigen, Middleton, Wisconsin, US) and used as template for the amplification of the dxs (1-deoxy D-xylulose 5-phosphate synthase ...
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bioRxiv - Microbiology 2021Quote: ... To extract DNA 10 ul of culture was added to 500 ul QuickExtract Solution (Epicentre) and treated 65 °C for 6 min followed by 98 °C for 2 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... and lysed in containing 200 μl of QuickExtract™ DNA Extraction Solution (Lucigen Cat. # QE09050) per well of 48-well plates ...
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bioRxiv - Genomics 2022Quote: ... the clean-up DNAs underwent the protocol SYGNIS TruePrimeTM WGA & Single Cell WGA Kits (Lucigen), instead of the REPLI-g single cell kit ...
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bioRxiv - Developmental Biology 2022Quote: ... Single cells were FACS sorted into 1 ul each of QuickExtract DNA Extraction Buffer (Lucigen). DNA was extracted by incubating cells for 10 minutes at 65°C followed by 5 minutes at 98°C ...
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bioRxiv - Microbiology 2020Quote: ... Germany). MasterPure DNA purification kit (Cat. No. MCD85201) was obtained from Epicentre (Middleton, WI, USA). GoTaq Green Master Mix (Cat ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from at least 104 harvested cells using the QuickExtract protocol (Lucigen). The target locus was then amplified by PCR with forward and reverse primers where at least one primer annealed outside the integration site (Supplementary Table 1) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted using the Epicentre MasterPure nucleic acid extraction kit (Epicentre, Madison, WI) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Biochemistry 2024Quote: ... The CRISPR/Cas9-edited mutants were validated by DNA extraction using QuickExtract solution (Lucigen, QER090150) followed by sanger sequencing prior cell line expansion28,38 ...
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bioRxiv - Genomics 2024Quote: ... the DNA & rRNA depleted RNA samples were treated with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). After a one-hour incubation ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Genetics 2023Quote: ... 400 ng of DNA was incubated with 5 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cells were resuspended in 50 µl of QuickExtract DNA Extraction Solution (Lucigen, Catalog #QE09050). Genomic DNA was extracted by treating the cells to the following protocol ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with 1 U/μl NxGen Phi29 DNA polymerase (Lucigen, cat# 302210-2), 250 μM dNTPs ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting DNA was transformed to an electro-competent ER2738 E.coli strain (Lucigen, 60522-1) and cultured on 2YTG (2% glucose ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL of purified DNA was transformed into 25 μL of Endura electrocompetent cells (Lucigen) following the manufacturer’s instructions with 2 mL of prewarmed recovery media ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL of purified DNA was transformed into 25 μL of Endura electrocompetent cells (Lucigen) following the manufacturer’s instructions with 2 mL of prewarmed recovery media ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Cell Biology 2020Quote: RNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, MC85200), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Plant Biology 2021Quote: ... then further purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, USA). 100 ng of DNA was used for qPCR ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Microbiology 2022Quote: ... It was then purified using the MasterPure Gram Positive DNA purification kit (Lucigen, Middleton, Wisconsin, USA) using the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
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bioRxiv - Systems Biology 2020Quote: RFP positive A549 VIM-RFP cells were harvested and DNA was extracted using QuickExtract (Epicentre, QE09050). Primers were designed for left homology arm and right homology arm junction PCR (left junction Fwd ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... gargle solution or sputum sample were mixed 1:1 with 2x QuickExtract DNA extraction solution (Lucigen) and heat inactivated for 5 minutes at 95°C ...
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bioRxiv - Genetics 2020Quote: For single cell clones or bulk sequencing genomic DNA (gDNA) was extracted by quick extract (Lucigen). PCR amplification was performed with LongAmp Polymerase (NEB ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. All samples were treated with Invitrogen TURBO DNA-Free kit to remove DNA contamination ...
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bioRxiv - Genomics 2024Quote: ... and cellular debris precipitated using steps and reagents from the MasterPure Yeast DNA Purification Kit (Lucigen). DNA was finally purified using the Monarch Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... they were further expanded and gDNA was harvested using Quick Extract DNA Extract Solution (Lucigen, QE9050). Excision and inversion clones were genotyped via ddPCR and Sanger sequencing (Table S66).
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bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... jirovecii-infected patients was extracted using the MasterPure Yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA). Total RNAs for P ...
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bioRxiv - Bioengineering 2020Quote: ... and had genetic material isolated via the MasterPure™ genomic DNA purification kit (Lucigen, Cat. No. MC85200). Genomic DNA was fragmented to 300 bp via Covaris sonication ...