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Citations for Agilent :
4901 - 4950 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Point mutations were made with complementary primers (listed below) in pTRCHisA-padi2 vectors using QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-C1-tubbyCT R332H and pEGFP-C1-tubbyCT Y343F were generated using QuikChange II XL Site-Directed mutagenesis kit (Stratagene, Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was determined by bleach agarose gel electrophoresis (Aranda et al., 2012) and further analyzed on a Bioanalyzer 2100 using the RNA 6000 Nano LabChip Kit (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... and extracellular acidification rate (ECAR) were measured in MSCs using a Seahorse XF24 Extracellular Flux Analyzer and kits from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Genetics 2021Quote: ... Quality assessment and library size estimation of the individual libraries was done using an HS DNA kit (Agilent, #5067-4626) in a Bioanalyzer 2100 ...
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bioRxiv - Developmental Biology 2021Quote: RNA integrity was assessed on an Experion bioanalyzer using a Bio-Rad Experion RNA High-Sensitivity kit or an Agilent 2100 bioanalyzer using a Bioanalyzer RNA 6000 Pico assay (Agilent). RNA concentrations were measured using a Qubit 4 fluorometer with a Qubit high-sensitivity RNA assay.
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (reripes samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Molecular Biology 2021Quote: ... after which quantification and qualification of purified band(s) were undertaken using the Agilent DNA 1000 kit and BioAnalyzer (Agilent). Illumina NGS was performed with 2×250 bp sequencing without fragmenting the amplicons ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Biophysics 2020Quote: ... by introducing a stop codon at position 475 (aa 233–474) using the QuikChange XL site-directed mutagenesis kit (Stratagene). STIM1 fragment 344-449 (CAD ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA integrity was assessed through the RNA 6000 Pico Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3μg RNA per sample was used for mRNA purification and library construction via the Truseq™ RNA Sample Prep Kit (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... Size distribution and concentration of the final products were then evaluated using the Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... and RNA 6000 Pico and RNA 6000 Nano kits –depending on the experiment– with a BioAnalyzer 2100 (Agilent Technologies Inc.). Libraries for each sample type were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
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bioRxiv - Molecular Biology 2022Quote: Concentrations were measured by Qubit RNA HS assay kit and the quality of RNA was analyzed on Tapestation D5000 HS (Agilent). Removal of rRNAs from isolated samples of IFNβ stimulated ESCs and MEFs was done following the protocol of Ribo-Zero rRNA removal kit ...
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... K22M mutation of the kinase dead PMVK mutant (PMVKmut) was generated using QuickChange Site-Directed Mutagenesis Kit (Agilent, Catalog # 200518). Lentiviral particles were used to constitutively express shPMVK (pLV[shRNA]-Hygro-U6> hPMVK[shRNA#4] ...
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bioRxiv - Biochemistry 2022Quote: ... P286R and V411L variants were obtained by carrying out point mutation to these clones using the QuikChange II site directed mutagenesis kit (Agilent). The oligonucleotides used to produce these mutations are shown in Table 2 ...
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bioRxiv - Biochemistry 2022Quote: The mutations S26C and K28C were introduced to SD-Aβ40 fusion protein19 via site-directed mutagenesis using a QuikChange Lightning Kit (Agilent, USA) following a modified protocol.22 The successful introduction of mutations was confirmed by sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2019Quote: FANCJ point mutations were generated by site-directed mutagenesis using a Quick Change II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... quality and quantity of both transcriptome and translatome samples were evaluated in an Agilent 2100 Bioanalyzer using Nano and Small RNA kits (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and concentration were checked using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Site-directed mutagenesis to generate the miCLIP-mutants was performed using the QuikChange II Site-Directed Mutagenesis Kit from Agilent as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, cat# 5067-4626) and quantified using KAPA Library Quantification Kits (KAPA Bio ...
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bioRxiv - Molecular Biology 2021Quote: RNA purified from polysomal fractioning (the reconstituted total mRNA and pooled polysomal RNA) was tested for quality using a RNA 6000 nano kit (Agilent) on a Bioanalyzer 2100 (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... 154 mutated to leucine) was generated using the QuickChange Site Directed Mutagenesis Kit according to the manufacturer’s protocol (Agilent #200519). The BirA Ligase containing plasmid was purchased (Addgene #64395) ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...
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bioRxiv - Cell Biology 2022Quote: ... of hPASMC were measured using Seahorse XF96 metabolic analyzer and Seahorse XF Cell Mito Stress Test kit (103015-100, Seahorse XF Technology, Agilent), following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Other variants containing single or multiple amino-acid changes in the spike gene from wildtype or D614G were made by mutagenesis using QuickChange lightning Multi Site-Directed Mutagenesis Kit (cat # 210515, Agilent) or via synthesis and cloning (Genscript) ...
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bioRxiv - Genomics 2020Quote: ... the amplified libraries were purified using a Qiagen MinElute PCR Purification Kit and eluted in 20 μl Elution Buffer before quantitation on an Agilent 4200 TapeStation (Agilent Technologies LDA UK Limited ...
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bioRxiv - Genomics 2020Quote: ... RNA quality was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and was checked on RNase free agarose gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Extracted RNA samples were then quantified and qualified using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, USA) with RIN number >7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quality of cDNA libraries were studied with BioAnalyzer DNA analysis (5067-1504, Agilent, Agilent High Sensitivity DNA kit, 5067-4626). Final DNA concentration was determined with Quantus Fluorometer (E6150 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant Mul DNA gyrases (Ala91Val or Asp95Gly) were generated from WT gyrA and gyrB using a QuickChange site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Final and cDNA libraries were then QCed on a bioanalyzer (Bioanalyzer High Sensitivity DNA kit: Agilent Technologies, Inc., 5067-4626) and qubit (dsDNA high sensitivity kit ...
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bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Constructs for BaxT182I and G179P were generated with a QuickChange site-directed mutagenesis kit (#210518; Agilent Technologies, Santa Clara, CA) on the pTYB1 WT-human Bax plasmid (Suzuki et al. ...
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bioRxiv - Genomics 2019Quote: The mutagenesis reactions to remove the Adenosine nucleotides near the cleavage sites were carried out using the QuikChange Site-Directed Mutagenesis Kit (Agilent). The mutagenesis DNA primers for the site mutation reactions are available in Supplemental Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... were quantified using KAPA library quantification kits (KAPA) and fragment size distributions were determined using a Bioanalyzer or Tapestation (Agilent). Sequencing was performed on a HiSeq 4000 (Illumina).
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bioRxiv - Plant Biology 2020Quote: ... Library validation included size and purity assessment with the Agilent 2100 Bioanalyzer and the Agilent DNA 1000 kit (Agilent Technologies). Samples were diluted to 10nM and pooled to create the 12X multiplexed DNA libraries ...
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bioRxiv - Biochemistry 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA concentration was measured with an RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... the kaiC36803 gene in the pGEX-kaiC36803 vector was modified by site directed mutagenesis using the Quick-Change Site-Directed Mutagenesis Kit (Stratagene) or Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2019Quote: ... Mutations in the miR-204 binding sites were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the mutated sequences were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...