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Citations for Agilent :
4851 - 4900 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 154 and 175 of Twist1 in the pMPB3 vector were performed using the QuikChange XL site-directed mutagenesis kit accorinding to the manufacturer’s instructions (Stratagene), using the following primers ...
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bioRxiv - Neuroscience 2024Quote: ... or 27 into methionine on histone H3.3A using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies #200521) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Extracellular flux analysis (Seahorse) was conducted using the Seahorse XF Real-Time ATP rate assay kit (Agilent, 103592-100). The day prior to the assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Telomeric FISH using a Cy3-labelled PNA probe was performed according to the manufacturer’s instructions (Telomere PNA FISH Kit/Cy3, Dako), all other methods including the cell cultivation followed the detailed protocols available in Supplementary Data of Altmanová et al ...
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bioRxiv - Microbiology 2024Quote: ... and the quality was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies) or Qubit (Thermo Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... gRNA-target-resistant constructs were generated by site directed mutagenesis using QuikChange II Site-directed mutagenesis kit (200521, Agilent) or Q5 Site-directed mutagenesis (E0554 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The final libraries were then quality-checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific) ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) ...
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bioRxiv - Immunology 2024Quote: ... PCR fragments were purified with Qiagen MinElute PCR Purification Kit and library concentration and quality were determined by Agilent High Sensitive DNA Kit and TapeStation ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... The library quality was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent #5067-4626). The libraries were sequenced at Source BioScience using Illumina NovaSeq 150bp paired-end reads.
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s instructions (Illumina #20020594) ...
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bioRxiv - Molecular Biology 2024Quote: ... The Agilent QuikChange Primer Design Program and the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, USA) were utilized to incorporate point mutations into the coding sequence of human GAT1 ...
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bioRxiv - Genetics 2024Quote: ... These mutations were introduced into pJW1 by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies, Cat #200523) to make pJW1-KR>DD and pJW1-KR>AA ...
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bioRxiv - Cell Biology 2024Quote: ... The Agilent SureSelect Strand Specific mRNA library kit was used as per the manufacturer’s instructions (Agilent, Santa Clara, CA). Library construction began with two rounds of polyA selection using oligo dT containing magnetic beads ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were run on an Agilent 2100 bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent, Diegem, Belgium). The bioanalyzer electropherograms were analyzed by Agilent 2100 Expert Software to determine the RNA quantity and quality ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotenone/Antimycin A was measured using the mitochondrial stress test kit (Agilent, USA). The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... Blood T cell fractions were estimated using TcellExTRECT using exons from the corresponding capture kit (Agilent custom V2 Exome) with a median coverage threshold set to five.
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bioRxiv - Physiology 2024Quote: ... for 24h then incubated in the Seahorse XF media (+/- 0.25mM succinate) and processed using the Cell Mito Stress Test Kit (#103015 Agilent).
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bioRxiv - Biochemistry 2024Quote: ... MicroRNAs (miRs) were polyadenylated and then re-verse-transcribed using the miR 1st-Strand cDNA Synthesis Kit (Agilent Technologies) according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... and the quality of the libraries was checked with a 2100 Bioanalyzer with a high-sensitivity DNA kit (Agilent). Libraries were sequenced using a P3 flow cell on a NextSeq2000 in 100 bp paired-end mode (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... the library preparations were sequenced on an Illumina Hiseq Ten platform with 150 bp paired end module.The panel kit was customized from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration of the final libraries was determined using the Tape Station High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... The quality and concentration of the RNA was assessed with Agilent RNA 6000 Pico Kit (Agilent, Cat#5067-1513) on Bioanalyser 2100 (Agilent) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA integrity was assessed with 1µl of each mRNA-purified sample using RNA 6000 Pico kit (Agilent, 5067-1513) on an Agilent Bionalyzer with RNA Integrity Number (RIN ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... The individual libraries were assessed using a Qubit™ and the Agilent Bioanalyzer dsDNA High Sensitivity Kit (Agilent Technologies). We sequenced at 2*76 base-pairs on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... of SspH1 was generated in the pcDNA3::2xHA-SspH1 backbone using the Quikchange II site-directed mutagenesis kit according to the manufacturer’s protocol (Agilent). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Two µL of each diluted sample were run using the HS Large Fragment 50kb Kit #DNF-464-0500 (Agilent) according to the manufacturer’s instructions on the 5200 Fragment Analyzer System # M5310AA ...
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bioRxiv - Biophysics 2024Quote: ... The resulting WGBS libraries were evaluated on a 2100 Bioanalyzer using Agilent High-Sensitivity DNA Kit (Agilent #5067-4626) and quantified via qPCR using the KAPA Library Quantification Kit (KAPA #KK4824) ...
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bioRxiv - Neuroscience 2024Quote: ... Quality and concentration of extracted RNA was assessed using a Tapestation 2100 with the High Sensitivity RNA kit (Agilent) for samples extracted form sorted somata and using a 2100 Bioanalyzer with the RNA 6000 Pico kit (Agilent ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Five micrograms of total purified RNA was enough for the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of the pCMV-S4 vector was performed using protocols and materials from QuickChange Lightning mutagenesis kit (Agilent). Nearly confluent monolayers of HEK293 cells in 12 well plates were transfected with either 0.5 μg of empty vector or 0.25 μg each of WT σ3 or σ3 K293T expression vector using 1.5 µl Lipofectamine 2000 according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Plamids for overexpression of the Nrd1 CID variants R133G and R133D were obtained using QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). Cyclin D1 KO was conducted by CRISPR/Cas9 genome editing (Xie et al. ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting pure DNA and RNA fractions were quality assessed using agarose gel electrophoresis and Bioanalyser RNA 6000 Pico Kit (Agilent). Nucleic acid concentration was quantified using Qubit dsDNA HS Assay and Qubit RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: The lyase deficient mutant of HMGA2 was generated by sequential site-directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent) of the first three arginines in the third AT-Hook domain ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid by digestion with AscI and MIuI and mutagenesis for RTEL1K48R was performed using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Cells were infected with lentiviruses expressing the indicated RTEL1 proteins and infected cells were selected with puromycin as previously described (Garzia et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples quantity (RNA Integrity Number = RIN) and quality were assessed using the Agilent RNA 6000 Pico Kit (Agilent #5067-1513) on the Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2020Quote: ... The pcDNA3-HA-Bbs5-S19A construct was prepared by mutating Ser19 of Bbs5 to alanine with pcDNA-HA-Bbs5-WT as template and site-directed mutagenesis kit (Stratagene). The primers were FWD1 (5′-CTTCGACGTGTCCGCACAACAGATGAAAAC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: Mito fuel flex test: Agilent Seahorse XF Mito Fuel Flex Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf) ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA were extracted and the quality of these samples was assessed (Agilent RNA 6000 Nano Kit Quick, Agilent Bioanalyzer 2100); RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Cell Biology 2020Quote: ... pWZL-Hygro-HDAC4PAM-ER pCW-Hygro-HDAC4PAM were obtained by sub-cloning a mutagenized HDAC4 (QuikChange Site-Directed Mutagenesis Kit, Agilent) into the linearized empty backbones by a restriction-based approach ...