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Citations for Agilent :
5101 - 5150 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared for each time-points and seady-state sample using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) automated on the NGS WorkStation (Agilent) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
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bioRxiv - Microbiology 2020Quote: ... Mutations to the NF-κB and IRF binding sites in pLTR(Sp1)-luciferase were generated using the QuickChange IIXL site-directed mutagenesis kit (Stratagene). Primers used for site-directed mutagenesis are listed in Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality of final single cell 5’ GEX libraries was assessed by Qubit quantification and fragment analysis (DNF-474 High Sensitivity NGS Fragment Analyzer Kit, Agilent). The sequencing was performed on a NextSeq500 device (Illumina ...
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bioRxiv - Genetics 2021Quote: ... RNA quality control (QC) was performed with the RNA 6000 Nano kit analysed using the 2100 Bioanalyzer Eukaryote Total RNA Nano assay (Agilent). cDNA libraries for sequencing were produced using the KAPA mRNA HyperPrep Kit for Illumina Platforms (Kapa Biosystems ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The BsaI site in the backbone of the binary destination vector pCAMBIA2300 was removed via the QuikChange Site-Directed Mutagenesis Kit (Agilent) (Hajdukiewicz et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the LdNT3-targeting construct shown in Figure S2D was modified with the QuikChange Site-directed Mutagenesis Kit (Stratagene, Lajolla, CA) using the primers listed in Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent) and single-end 125bp sequences generated on an Illumina Hi-seq 2500.
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bioRxiv - Molecular Biology 2020Quote: The quality of 500 ng bisulfite-treated mRNA (see above) was assessed using Agilent RNA 6000 Pico Kit (Agilent, #NC1711873), and then subjected to NGS libraries preparation using VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme) ...
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bioRxiv - Molecular Biology 2020Quote: ... F561A and K568A) were generated by site directed mutagenesis using the Quick-Change Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... We measured the size distribution and concentration of each well in 96-well plate using Fragment Analyzer High-sensitivity NGS kit (Agilent). We normalized the concentration of cDNA of each well to a concentration of 0.4 ng/µL ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and RNA quality was assayed on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Pico Kit (Agilent Technologies, USA). Libraries were built using TruSeq Stranded Total RNA Library Prep Kits (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Substitution mutations in the IDR were constructed by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The pBad-Gfp-IDRFtsZ was constructed by cloning the coding sequence of Gfp-IDRFtsZinto the NheI and HindIII sites on the arabinose inducible vector ...
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bioRxiv - Cell Biology 2021Quote: ... and were mutated by deleting 6-7 base pairs using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). The primers used for mutagenesis are listed in Supplementary Table S6 ...
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bioRxiv - Cell Biology 2021Quote: ... The quantity and quality of RNA samples were analyzed using Agilent RNA 6000 Nano kit with the bioanalyzer from Agilent. RNA samples with RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... All mutant constructs used in this study were generated from this construct either by a QuickChange II XL kit (Stratagene) or a Q5® Site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated and purified using the Absolutely RNA Miniprep Kit (Cat# 400800, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol for extremely small samples ...
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bioRxiv - Neuroscience 2021Quote: ... KCNQ2 mutants were generated by site-directed mutagenesis using a QuikChange kit according to manufacturer’s protocol (Stratagene, San Diego, CA). cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... after quality control using the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA), poly-T oligo-attached magnetic beads were used to purify poly-A containing mRNA ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... quality of libraries was assessed using a high sensitivity Small DNA Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... with an average fragment length of 625 bp (ranging from 300 to 2000 bp, measured on Bio Analyzer High Sensitivity DNA Kit, Agilent). The prepared library was sequenced on Illumina NovaSeq 6000 Sequencing system with S4 flow cells ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA library quality was quantified by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent, Santa Clara, CA). Library was sequenced as 101 bp paired-end on Illumina NovaSeq6000.
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bioRxiv - Neuroscience 2020Quote: ... An AgeI site was inserted into exon 1 of Cacna1b by mutagenesis using a QuickChange II XL site-directed mutagenesis kit (Stratagene) with primers 1-For and 1-Rev ...
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bioRxiv - Immunology 2020Quote: ... The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA kit (Agilent), diluted and pooled in multiplex sequencing pools ...
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bioRxiv - Molecular Biology 2022Quote: ... Final size selected libraries were checked for quality on the Agilent BioAnalyzer with High Sensitivity DNA analysis kit (5067-4626, Agilent) to confirm proper size selection ...
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bioRxiv - Molecular Biology 2022Quote: ... The final libraries were assessed on a fragment analyzer using the High Sensitivity NGS Analysis Kit (Agilent, DNS-474-FR) and sequenced using the NextSeq550 Illumina platform (75-bp paired-end reads) ...
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bioRxiv - Neuroscience 2022Quote: ... following the manufacturer’s protocol and RNA quality was assessed using the RNA 6000 Pico Kit on a 2100 Bioanalyzer (Agilent Technologies). RNA sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA libraries were quantified using Fragment Analyzer 5300 (Advanced Analytical) kit FA-NGS-HS (Agilent, Cat#: DNF-474-1000) and Spectramax M2 (Molecular Devices ...
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bioRxiv - Genetics 2022Quote: ... This variant was deemed a SNP without functional consequences but subsequently corrected and other missense variants were generated by QuickChange Multi Site-Directed Mutagenesis Kit (Agilent). WT and missense variant cDNAs were transferred from pDONR223 to MSCV_IP_N terminal-HA-FLAG vector using Gateway recombination (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Library size and quality was checked using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, #5067–4626) or the Fragment Analyzer CRISPR discovery kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The coding exons and flanking intronic regions were enriched with the SureSelectXT Low Input All Exon v7 kit (Agilent Technologies) followed by dual index ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA-VP4-KpnI/BamHI was built by insertion of point mutations in pcDNA-VP4-SA11 using the QuikChange site-directed mutagenesis kit and protocol (Agilent) to insert KpnI and BamHI restriction sites in VP4 ...
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bioRxiv - Genomics 2022Quote: ... The quantity of extracted HMW DNA was evaluated with the HS Qubit DNA kit and the fragment profile and overall quality was assessed with the Femto Pulse instrument (Agilent). Pacific Biosciences (PacBio ...
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bioRxiv - Immunology 2022Quote: ... and quality assessed using the RNA 6000 Pico Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... Mutagenesis was carried out to introduce the H840A mutation into eSpCas9(1.1) variant using pJSC114 plasmid (addgene, 101215) and QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518). eCas9 nickase was expressed using the mutagenesis-modified pJSC114 plasmid ...
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bioRxiv - Genetics 2022Quote: ... An AgeI site was introduced upstream of the SV40 polyA signal and the BamHI site downstream of the SV40 polyA signal was deleted using the QuikChange Lightning site-directed mutagenesis kit (Agilent). Synthesized oligos were amplified with 0.05pmol of template per 50μL PCR reaction for seven cycles using MPRA library amplification primers ...
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bioRxiv - Immunology 2022Quote: Seahorse assays were performed according to the manufacturer’s instructions to analyze glycolysis metabolism using the Seahorse XF Glycolysis Stress Test Kit (Agilent Technologies). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Quality control of cDNA and scRNA-seq libraries were performed using the Bioanalyzer High Sensitivity kit (Agilent Technologies Inc., CA). Libraries were sequenced using Novaseq 6000 (Illumina Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... Mutations were inserted into MP and GFP:MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S5.
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bioRxiv - Immunology 2022Quote: ... Slides were washed in PBS and incubated with horseradish peroxidase (HRP) conjugated secondary antibody included in the peroxidase substrate kit (Dako EnVision + System HRP labelled polymer anti-mouse or anti-rabbit IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... V37W) from pLOC-NT5C2 plasmid by site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) according to manufacturer’s guidelines.
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bioRxiv - Cell Biology 2022Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2022Quote: ... Quantitation and quality assessment of each of the 12 libraries were performed on 2200 Tapestation analyzer using the High Sensitivity D1000 ScreenTape kit (Agilent).
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bioRxiv - Biochemistry 2019Quote: Mutants were generated for each domain by introducing single point mutations using the Quick change site-directed mutagenesis kit (Stratagene). The primer sequences used for mutagenesis are listed in the supplementary S1 Table ...