Labshake search
Citations for Agilent :
3801 - 3850 of 3851 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Aliquots of 1 μL were injected into the column (HP5-MS, 30 m, 250 μm ID, 2.5-μm film, Agilent Technologies, Palo Alto, CA, USA) using a pulsed split-less mode and He as carrier gas at a flow rate of 1 mL min-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... precipitates were diluted in water and 1 μg of pure DNA was checked for its quality and quantity on Genomic DNA ScreenTape (Agilent, cat. no. 5067-5365) using TapeStation 4150 (Agilent).
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bioRxiv - Biochemistry 2024Quote: ... method was set up for detection of methionine and its oxidation products (Table 1), using a Sciex 6500 QTrap mass spectrometer (Framingham, MA, USA) coupled to an Infinity 1290 LC system (Agilent, Santa Clara, CA, USA). Samples were stored on the autosampler tray at 5 °C prior to injection ...
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bioRxiv - Cell Biology 2024Quote: ... and then washed with Roswell Park Memorial Laboratory medium for XF (Roswell Park Memorial Institute medium; RPMI, 103576-100, Agilent Technologies; containing 1% FBS) for XF before analysis ...
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bioRxiv - Microbiology 2024Quote: ... Sample DNA were serially diluted 1:10 in sterile nuclease-free water to dilute the concentration of any PCR inhibitors using a liquid handler (Agilent, Agilent Velocity 11 Vprep). 16S rRNA concentrations from embryonic tissue are reported from undiluted DNA as its concentration was approaching the limit of detection in diluted reactions ...
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bioRxiv - Neuroscience 2024Quote: ... Immunohistochemistry (IHC) for HLA-DR and OPCs was carried out by incubating with anti-human HLA-DR (1:200; Agilent, M0746, TAL.1B5 clone) and anti-human NG2 (1:25 ...
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bioRxiv - Neuroscience 2024Quote: ... carb3 clone) and the specific molecule of antigen presenting-cells HLA-DR (low or absent in M-MDSCs; 1:100, Agilent M076, TAL.1B5 clone). After incubating the tissue with the primary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent, Santa Clara, CA, USA) (m/z 622 ...
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bioRxiv - Cell Biology 2024Quote: ... The TIMS elution voltage was linearly calibrated to obtain 1/K0 ratios using three ions from the ESI-L Tuning Mix (Agilent, Santa Clara, CA, USA) (m/z 622 ...
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bioRxiv - Evolutionary Biology 2024Quote: A phage display PAI-1 variant library was constructed on the I91L PAI-1 background using error prone PCR as previously described (7, 8) with the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) using primers that preserved the AscI and NotI restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies, Santa Clara, CA, USA) using the Neon Transfection System (Thermo ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat. X0943 - Agilent Technologies, Santa Clara, CA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and quality were assessed by means of a 1% agarose electrophoresis gel and in Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... a high-power resistor of 1 Ω was placed in series with the coils and voltage drop across the resistor was observed with an oscilloscope (Agilent Technologies, Santa Clara, CA) during PEMF exposure period ...
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bioRxiv - Genomics 2020Quote: ... and assessed for quality using a 1 % (w/v) pulse-field gel electrophoresis and with genomic ScreenTape on the TapeStation 2200 platform (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA). A solution of fatty acid methyl esters (C8 to C30 ...
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bioRxiv - Cell Biology 2020Quote: ... and pom1521-200-GFP (pKBB520) fusions were generated by amplifying sGFP from pJK19-1 using PCR with PfuUltra™ HF (Agilent, Inc. Santa Clara, CA) and primers that generated chimeric Pom152-sGFP PCR products for homologous recombination in yeast ...
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bioRxiv - Microbiology 2021Quote: ... The integrity of RNA extracts was evaluated using gel electrophoresis on 1% agarose in 1x TBE and the Agilent 2200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA). Libraries were prepared from three samples per treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis. Membranes were incubated with primary antibodies (Suppl. Table 1) overnight and probed with respective secondary antibodies (Dako-Agilent, Santa Clara, CA, USA) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at room temperature for 1 hour and analyzed using gas chromatography-mass spectrometry (6890N GC, coupled to 5873N Mass Selective Detector; Agilent Technologies, Santa Clara, CA, USA). Statistical significance was assessed by mixed linear model with pBMI ...
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bioRxiv - Molecular Biology 2023Quote: Optimal temperature was measured using the enzyme activity assay described above in 1 mL cuvettes using a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA) at 22 °C and temperatures from 30 °C to 50 °C with 5 °C increments using a circulating water bath ...
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bioRxiv - Neuroscience 2023Quote: ... IDH 1 (R132H) (QM002; quartett GmbH, Potsdam, Germany), ATRX (BSB-108; Bio SB, Goleta, CA, USA) and Ki67 (MIB1; Agilent Technologies, Santa Clara, CA, USA) were routinely performed according to standard protocols.
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bioRxiv - Cancer Biology 2023Quote: ... IHC was performed on an automated Ventana Discovery Ultra staining system using primary antibodies recognizing Ki-67 (M7240, DAKO, mouse monoclonal, clone MIB1, 1:50) for 30min ...
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bioRxiv - Microbiology 2023Quote: K65R and M184V mutations in RT were generated by site-directed mutagenesis on an HXB2 or LAI HIV-1 backbone using the QuikChange XL site-directed mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... Desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled to PEGASUS 4D mass spectrometer (LECO Corporation) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Immunodetection was carried out by blocking PVDF membranes in 5% (w/v) fat-free dried milk powder followed by incubation overnight in rat anti-FLAG antibodies diluted 1:500 (Agilent Technologies, Santa Clara, CA, USA). After a brief rinse in Tris-buffered saline with Tween™ 20 detergent (TBST) ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent, catalog no. DNF-474-1000). Genes with low expression value (mean FPKM value<0.5 ...
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bioRxiv - Microbiology 2022Quote: ... CFSs were diluted in SDW (ratio 1/20) and injected into a high-performance liquid chromatography (HPLC) system (Agilent 1100 Series 203 HPLC System, Agilent Technologies, Palo Alto, CA, USA), equipped with a diode array and quaternary pump ...
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bioRxiv - Cancer Biology 2022Quote: ... explants treated with BrdU substrate for 24 hours prior to fixation as described in Section 6.3.1) (DAKO, Cat. M0744; 1:50 overnight at 4 °C), cytokeratin (DAKO ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Microbiology 2024Quote: ... Twenty-microliter reactions were prepared with 1×Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc. ...
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bioRxiv - Microbiology 2023Quote: Headspace CO2 of fungal incubations (1 mL) were measured for CO2 concentration on a Hewlett Packard HP 5890 Series II GC (Agilent Technologies, Inc, Santa Clara, USA) coupled with a Thermal conductivity detector (TCD) ...
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bioRxiv - Molecular Biology 2023Quote: ... Liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) was performed using a C3 column (Agilent Poroshell 300SB-C3, 1 x 75 mm, 5 µm) with a gradient of 5% to 95% acetonitrile (0.1% v/v formic acid ...
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bioRxiv - Microbiology 2023Quote: ... the cells were washed once with the same buffer and incubated with FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody from DAKO (dilution 1:40 in BP) for 1 hr at 4°C on ice with shaking ...
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bioRxiv - Cell Biology 2024Quote: ... the size distribution of each ChIP DNA sample was assessed by running a 1 µL aliquot on Agilent High Sensitivity DNA chip using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The concentration of each DNA sample was determined using a high sensitivity Quant-iT™ dsDNA Assay Kit and a Qubit Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... split flow of 30 mL · min-1) into a gas chromatograph equipped with an FID detector and headspace autosampler (Agilent 7890 series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50 m by 320 μm by 1.05 μm column ...
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bioRxiv - Microbiology 2021Quote: ... split flow of 30 mL min-1) into a gas chromatograph equipped with a FID detector and headspace autosampler (Agilent 7890 Series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50m × 320 µm × 1.05 µm column ...
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Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switchingbioRxiv - Microbiology 2021Quote: ... split flow of 30 mL min−1) into a gas chromatograph equipped with an FID detector and headspace autosampler (Agilent 7890 Series; Palo Alto, CA, USA). Analytes were separated on a HP-5 capillary column (50 m × 320 μm × 1.05 μm column ...
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bioRxiv - Neuroscience 2021Quote: Samples were removed from the −80°C freezer and allowed to warm to room temperature then vortexed for approximately 1 minute before being placed into the autosampler and held at 24°C (Agilent 1100 series autosampler, Palo Alto, CA) for the LC/MS/MS analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... derivatization followed by gas chromatography – electron-ionization-time-of-flight mass spectrometry (GC-EI-TOFMS) in split mode (split 1:50, Agilent straight split liners with glass wool) was used15 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.6) and then incubated with the secondary antibody (goat anti-mouse IgG antibodies coupled to horseradish peroxidase from DAKO at 1:5000 in Roti®-Block) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Milk powder and 0.5% Tween-20) and incubated with HRP-conjugated secondary antibody (diluted 1:2000 in Blocking buffer, rabbit α-mouse DAKO #0260, swine α-rabbit DAKO # P0217) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... For intact protein analysis,10 μL of desalted protein (∼1 mg/ml) was injected onto a Zorbax 300Å Stable Bond C8 column (Agilent Technologies, 4.6 x 50 mm, 3.5 μM) for reversed phase separation at 60°C and 0.4 mL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... fixed tissues were embedded in paraffin and 4 mm sections were histologically processed for hematoxylin-eosin staining and immunohistochemistry using anti-human Ki67 antibody (DAKO #M7240, 1:75, 30 min at 37°C). All these procedures were performed using standardized protocols by Atrys Health S.A.