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Citations for Agilent :
3601 - 3650 of 3851 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... was performed to remove adapter dimers and 1 μl of the cleaned pool was visualised using the Agilent High Sensitivity DNA Kit for Bioanalyzer 2100 (Agilent Technologies) to confirm adapter-dimer removal.
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bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... frozen femur sections were blocked with 3% BSA and 5% goat serum in PBS, incubated with Cgrp antibody (rabbit polyclonal, abcam #ab47027, 1:300 in DAKO antibody diluent (Agilent, #S0809)) ...
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed a further 3 times in TBS-T for 5 minutes and developed with DAB diluted at a 1:50 ratio in DAB-chromagen (Agilent; #K3468) until appearance of brown staining ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The putative ESRRA binding site 1 was mutated using PCR-based site-directed mutagenesis and QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518) to obtain mutated constructs pGL3-Leptin mut 1-luc.
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bioRxiv - Molecular Biology 2023Quote: ... desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies) coupled to a 5975B mass spectrometer (Agilent Technologies) ...
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bioRxiv - Plant Biology 2023Quote: ... 500 mg (1 mL) of sample powder was transferred immediately into a 20-mL headspace vial (Agilent, Palo Alto, CA, USA) that contained an NaCl-saturated solution ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies for 1 h at RT (Goat-anti-Rabbit-HRP, Goat-anti-Mouse-HRP, both Dako Denmark). Marker lanes were incubated with Streptavidin-biotinylated-HRP (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Plant Biology 2023Quote: ... the pET-28a plasmid bearing WT CG-1 domain or its six-point mutants (M1 to M6) were transformed into BL21-Codon Plus (DE3)-RIL (Stratagene, USA) cells ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-CD61 immunohistochemistry (Monoclonal Mouse Anti-Human, Citrate Buffer HIER, dilution 1:75, Clone Y2/51, Dako Omnis, Code Number: M0753) was also employed to evaluate the presence of platelet-enriched microthrombi ...
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bioRxiv - Neuroscience 2022Quote: A one-in-six series of coronal brain sections from each mouse were stained on slides using a modified immunofluorescence protocol22 for the following antibodies - astrocytes (rabbit anti-GFAP, 1:1000, Agilent Z0334), microglia (rat anti-mouse CD68 ...
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of the library was hybridized to the Agilent SureSelect XT Human All Exon V4 probes (Agilent Technologies 5190-4634). The targeted exon fragments were captured by Streptavidin beads (Fisher 65602) ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Evolutionary Biology 2024Quote: The prepared carbon dots were studied using UV−vis (1 cm cell quartz cuvette) in a Cary 100 UV−vis spectrophotometer (Agilent Technologies) in DI water ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed three consecutive times with Tris buffer (5min) and 1:10000 diluted donkey- or goat-anti-rat-HRP (Agilent Technologies) or 1:25000 diluted goat-anti-mouse-HRP (BioRad ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... After irradiation the sample was combined with 10 µl of formic acid 1 % and 10 µl analyzed by LC-MS using an Ultimate 1100 HPLC system (Agilent Technologies) connected to an Amazon Speed ETD ion-trap mass spectrometer (Bruker Daltonics) ...
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bioRxiv - Immunology 2023Quote: ... pH 7.4) for 1 h without CO2 prior to measurement of O2 consumption and extracellular acidification rate (ECAR) by XFe24 (Seahorse Bioscience) with sequential addition of 1 μM of oligomycin ...
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bioRxiv - Evolutionary Biology 2023Quote: The total RNA extracted from the floral meristem of species listed in Supplementary Table 1 were checked for quality using RNA ScreenTape (Agilent Technologies) and Qubit 2.0 fluorometer was used for determining the concentration ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... neurons were incubated for 1 hour in CO2-free incubator in Agilent Seahorse DMEM assay medium (Agilent Technologies, cat# 103575-100) supplemented with 1 mM sodium pyruvate (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Biochemistry 2023Quote: ... blots were incubated for 1h at room temperature with a horse radish peroxidase-labeled secondary antibody (anti-rabbit, Dako; 1/1000), and revealed by ECL chemiluminescence (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 mM glucose (for the Cell Mito Stress Test Kit) or 1 mM glutamine (for the Glycolysis Stress Test Kit) was used as the assay medium (103681-100; Agilent Technologies). Briefly ...
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bioRxiv - Pathology 2023Quote: ... EGTA 1 mM and 1mg/ml bovine serum albumin) supplemented with respiratory substrates (glutamate/malate) and 50 nM MitoXpress (Agilent, USA). Each well was loaded with 10μl respiratory substrates ...
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bioRxiv - Microbiology 2022Quote: ... ΔNef ANCH3 (HIV-1 ANCH3 IND116A) was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent #200522), for integrase (IN ...
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bioRxiv - Biochemistry 2022Quote: ... Point mutations (I91F and K176E) were introduced using the QuickChange II XL Site-Directed Mutagenesis Kit (SI Table 1; Agilent Technologies). The full-length sequence of the PAI-1 constructs was confirmed by Sanger sequencing (SI Table 1) ...
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bioRxiv - Bioengineering 2022Quote: Cells were dissociated from differentiation wells and seeded at 8 × 104 cells well−1 in CM support medium on Matrigel-coated XFe96 monolayer plates (Agilent Technologies). Culture medium was changed the next day to the well’s respective treatment ...
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bioRxiv - Cell Biology 2022Quote: ... The sulfo-SANPAH solution was placed on gels and activated using 1 Joule of 254 nm UV light (Stratagene UV crosslinker). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice and incubated for 1 h at 37°C in the non-CO2 chamber with 180 μl of the assay medium (Agilent; 103575) supplemented with L-glutamine (2 mM ...
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bioRxiv - Cell Biology 2022Quote: ... treated cells were then equilibrated in seahorse XF base medium supplemented with 1g/L glucose at 37°C in non-CO2 incubator for 1 hour prior to analysis using a Seahorse XF24 Extracellular Flux Analyzer (Seahorse Bioscience).
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bioRxiv - Immunology 2022Quote: ... The biotinylated antibodies were detected with HRP-conjugated streptavidin (for two replicate dilution series) and with a 1:1000 dilution of HRP-conjugated anti-mouse antibody (Dako, P0260) (for another two replicate dilution series) ...
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bioRxiv - Genomics 2022Quote: ... in a final volume of 50 μL and were diluted to 1.5 ng uL-1 to be measured in the High-Sensitivity ScreenTape D1000 reagents (Agilent Technologies, Germany). We included blank controls in all laboratory processes together with our samples ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... containing 1 µM sulfadimethoxine as an internal standard before analyzing 15 µL on a 1290 Infinity II UHPLC system (Agilent Technologies) coupled to an ImpactII ultra-high resolution Qq-TOF mass spectrometer (Bruker Daltonics ...
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bioRxiv - Microbiology 2023Quote: ... all tissue section slides were treated with a mouse monoclonal Anti-Human IgG p27KIP1 (Clone SX53G8.5 dilution 1:100; Code M7203; Dako Cytomation, Denmark) that cross-reacts with p27kip1 using a protocol described previously [24] ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
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bioRxiv - Plant Biology 2023Quote: ... allowing a sub-sample (∼1 μg) into the pyrolizer of the GC/MS apparatus (Agilent, 7890A/5975C, Agilent Technologies AB, Sweden). Following pyrolysis ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: The tissue sections used for the Visium spatial experiments were stained with DAPI Fluoromount G and Ki67-Cy5 (1:100 DAKO #M7420). The individual channels corresponding to DAPI and Cy5 were scanned and saved as TIFF files ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR was performed using the 1-Step Brilliant II SYBR Green quantitative RT-PCR master mix kit (Agilent Technologies) on a Bio-Rad C1000 Thermal Cycler CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 was scored on the glass slide of whole tissue sections by a breast pathologist (BC) according to the Ki-67 IHC MIB-1 pharmDx (Dako Omnis) Interpretation Manual for Breast Carcinoma ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Plant Biology 2024Quote: ... Powdered samples (1 g) were weighed and transferred immediately to a 20 mL head-space vial (Agilent, Palo Alto, CA, USA), containing NaCl saturated solution to inhibit potential enzyme reactions ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with Alexa Fluor®-conjugated secondary antibodies for 1 hour at room temperature and mounted on slides using DAPI-containing mounting media (Dako). Images were acquired using a Zeiss LSM 800 series confocal microscope at the Yale CCMI facility ...
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bioRxiv - Plant Biology 2024Quote: ... Fatty acid methyl esters (1 ml) were injected onto a DB-23 capillary column (60 m · 0.25 mm · 0.25 mm (Agilent Technologies, United States). A mix of FAME standards was used as references for peak identification by retention times ...