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Citations for Agilent :
3451 - 3500 of 3851 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Plant Biology 2022Quote: ... adsorption at 45°C for 5 minutes and desorption at 250°C for 1 minute onto a DB-624 capillary column with 60m x 0.25mm x 1.40μm film thickness (Agilent Technologies, Böblingen, Germany). The GC temperature was programmed as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-GFAP immunohistochemistry (Polyclonal Rabbit Anti-Human, Proteinase K enzymatic antigen retrieval, dilution 1:1000, DAKO Omnis, Code Number: GA524) was employed to assess reactive astrogliosis ...
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bioRxiv - Molecular Biology 2023Quote: ... we used horseradish peroxidase (HRP)-conjugated isotype-specific anti-rabbit IgG or anti-mouse IgG (1:10000; DAKO, Cambridgeshire, UK) secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Bone marrow was flushed out using a syringe and 1 ml of PBS + 5% FBS and filtered through a 40 μm strainer (Dako). Cells were counted and analyzed immediately ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse, Agilent) for measuring the extracellular acidification rate (ECAR ...
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bioRxiv - Biochemistry 2022Quote: ... for a 200 μL sample of water in a well of a 96-well microtiter plate using the microplate reader and an 800 μL sample of water in a 1 cm quartz cuvette using a UV−Vis-NIR spectrophotometer (Cary 7000, Agilent) in the equation below ...
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bioRxiv - Plant Biology 2024Quote: ... 500 mg (1 mL) of the powder was transferred immediately to a 20 mL head-space vial (Agilent, CA, USA), containing NaCl saturated solution ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-GGCT (16257-1-AP; proteintech) antibodies were used for immunodetection on rehydrated sections pretreated with Target Retrieval Solution (S1699; DAKO). The Envision™+ system (HRP/DAB ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed overnight at 4 °C with rabbit monoclonal anti-PSyn EP1536Y diluted 1:320,000 in DAKO antibody diluent (Agilent #S0809). After three 5-min washes with PBST ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Biochemistry 2024Quote: ... For all experiments the TIMS elution voltages were calibrated by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 hour before being placed into a Seahorse XFe24 Analyzer (Agilent). For oxygen consumption rate (OCR ...
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bioRxiv - Neuroscience 2023Quote: ... The steroid extraction was performed as earlier described for plasma [39] with a further modification of the CSF analysis to encompass a different size of solid-phase extraction columns (100 mg Bond elute C18 solid-phase extraction cartridges; 1 ml; Agilent) and therefore corresponding volume changes for conditioning (1 ml MeOH followed by 2×1 ml dH2O) ...
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bioRxiv - Microbiology 2023Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...
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bioRxiv - Microbiology 2022Quote: We extracted extracellular metabolites from the seawater matrix using Bond Elut PPL cartridges (1 g/6 ml sized cartridges, Agilent) following the protocol of Dittmar et al ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 minutes with the rabbit anti-human HES1 antibody diluted 1:100 in a low background diluent (Dako). A linker rabbit was used to amplify the signal ...
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bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were incubated overnight with the following primary antibodies: rabbit polyclonal anti-glial fibrillary acidic protein (GFAP) (1:6,000, DAKO, UK), rabbit polyclonal anti-allograft inflammatory factor 1 (IBA1 ...
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... Membranes were then washed in TBST and incubated with the secondary HRP-conjugated antibodies: goat anti-rabbit (1∶1000, P0448, DAKO) or polyclonal anti-mouse (1∶2000 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with 1x PBST for 5 min and incubated with secondary antibodies anti-mouse-HRP (1:10,000) (Agilent Dako, #P0447) and anti-rabbit-HRP (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality was determined by running 1 μL of the sample on a BioAnalyzer RNA Nano chip (Agilent, No. 5067-1511) for RIN ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... The percentage of the genome fragmented to sizes between 1 – 4 kb was then empirically measured by fragmenting 1 µg of genomic DNA followed by quantification on a Genomic DNA ScreenTape assay (Agilent). Hpy166II fragments 41% of the genome to within the target size range ...
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bioRxiv - Plant Biology 2023Quote: CDS sequences from PhDEF and PhGLO1 were amplified from Petunia x hybrida R27 inflorescence cDNAs with primers MLY2382/MLY2383 and MLY2384/2385 respectively (Supplementary Table 1) and cloned into the in vitro translation vector pSPUTK (Stratagene) by NcoI/XbaI restriction ...
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were incubated for 60 min at room temperature with primary antibodies (Monoclonal Mouse Anti-Human Ki67, clone MIB-1, Dako North America Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... we calibrated the TIMS elution voltages by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...