Labshake search
Citations for Agilent :
3501 - 3550 of 3851 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA integrity was confirmed by agarose (1% w/v) gel electrophoresis and by microfluidic electrophoresis using a Bioanalyzer 2100 (Agilent Technologies ...
-
bioRxiv - Plant Biology 2023Quote: ... the first-strand complementary DNA (cDNA) was synthesized from 1 μg of RNA using oligo (dT) primers with Reverse Transcriptase (Agilent). Quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
-
bioRxiv - Genetics 2023Quote: ... Membranes were labelled with primary antibody for 1 hour at room temperature or overnight at 4°C followed by incubation with HRP-conjugated secondary antibodies (Dako). Membranes were developed using the Western Lightning ECL system (Perkin Elmer) ...
-
bioRxiv - Microbiology 2023Quote: Log phase cultures of cells grown without inducer were diluted to an OD600 of 0.05 and grown in 96-well plates for 24 h at 30°C with constant shaking on a BioTek Cytation 1 plate reader (Agilent), measuring the absorbance at 600 nm every 30 min for three technical replicates for each strain ...
-
bioRxiv - Cell Biology 2023Quote: ... Kidneys were equilibrated at 37°C for 1 h in a non-CO2 incubator and then assayed on a Seahorse XFe24 Analyzer (Agilent) following a 12-min equilibration period ...
-
bioRxiv - Cancer Biology 2023Quote: ... Patient-matched primary tumor tissues and PDTOs were immunostained for diagnostic NSCLC markers with antibodies against cytokeratin (CK)7 (mouse, M7018, 1:100; Dako), CK5/6 (mouse ...
-
bioRxiv - Cell Biology 2023Quote: ... siRNA-resistant versions of Cofilin-1 have been obtained by mutating 6 bp of the siRNA-targeting sequence using QuickChange (Agilent). Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche) ...
-
bioRxiv - Immunology 2023Quote: ... 10 µg/mL of either mouse anti-human LAIR-1 (clone Dx26; purified in house) or mouse isotype control IgG1 (Dako) diluted in PBS+1% BSA buffer were incubated overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... For staining of macrophages and platelets the aortic tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After blocking ...
-
bioRxiv - Plant Biology 2024Quote: ... A splitless injection volume of 1 μL was injected onto an HP-5 column with 5% phenyl methylpolysiloxane stationary phase (Agilent) with dimensions of 30 m x 0.25 mm x 0.25 μm ...
-
bioRxiv - Cell Biology 2024Quote: ... One aliquot was solubilized with 1% SDS to determine protein content and the other aliquot was mixed with 1 mL 30% HNO3 and subjected to ICP/MS 8900 (Agilent). Data was normalized by the corresponding protein concentration.
-
bioRxiv - Cancer Biology 2024Quote: ... These tumor sections were stained at the Histology core (Henry Ford Health) according to the manufacturer’s protocols (Ki-67 IHC MIB-1, Dako Omnis). Proliferating cells were immunostained with FLEX monoclonal mouse anti-human Ki-67 (Catalog No ...
-
bioRxiv - Biochemistry 2024Quote: The HAdV5HVR7 vectors (“1st generation” vectors) were obtained by cloning the gene of interest into the pAdEasy-1 plasmid system from Agilent Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 mM Seahorse XF L-glutamine solution and 1 mM Seahorse XF pyruvate solution (103577-100, 103579-100 and 103578-100, all from Agilent) and cells were kept in a CO2 free incubator for 45 min prior to starting the Cell Mito Stress Test ...
-
bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
-
bioRxiv - Neuroscience 2024Quote: Cells were counted and plated at 1 × 105 (mouse astrocytes or neurons) cells per well in a Seahorse XF24 Cell Culture Microplate for all experiments (Agilent). Cells were then treated with indicated inhibitors or agonists in each experiment for 20 h ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 h before being placed into a Seahorse XFe24 Analyzer (Agilent). For OCR and ECAR mitostress test experiments ...
-
bioRxiv - Immunology 2024Quote: ... after which live cell imaging analysis was carried out for 30 minutes at a rate of 1 frame per 30 or 40 seconds using BioTek LION Heart XL (Agilent) or TomoStudio (Tomocube ...
-
bioRxiv - Molecular Biology 2024Quote: ... which was amplified in 15 PCR cycles using Herculase II Fusion DNA polymerase in a 100-μl reaction mixtures (1x Herculase II reaction buffer, 1 mM dNTPs, 200 pM Agilent library ...
-
bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
-
bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
-
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used in this study: rabbit anti-mouse immunoglobulins/HRP (Agilent Cat# P0260, RRID:AB_2636929, 1/10000), goat anti-rabbit IgG (H+L ...
-
bioRxiv - Cell Biology 2024Quote: MBP-MPS1104-330/WT and MPS1104-330/4A were obtained by over-expression in 1 L culture of BL21CodonPlus(DE3)-RIL cells (Agilent Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... infected with VSVΔM51-GFP at the indicated MOIs and treated with 0.3-3µM CBL0137 (or media alone) 1 hpi using the Agilent Bravo liquid handling system (Agilent, CA, USA). 24 hpi ...
-
bioRxiv - Neuroscience 2024Quote: ... The antigen retrieval process involved incubating the sections with DAKO Target Antigen Retrieval Solution (diluted 1:10; Agilent Technologies, USA) in a water bath at 90 °C for 45 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... membranes were immunoblotted for 1 hour with secondary antibodies (1:5,000) anti-mouse or anti-rabbit linked to peroxidase (reference number: P0260 and P0217, respectively, Dako Agilent). Signal for (3xFLAG)TamL was generated using Clarity™ Western ECL substrate chemiluminescence reagent (BioRad) ...
-
bioRxiv - Physiology 2024Quote: ... or 32°C (naked mole- rat) and the O2 level was set to 1%O2 using the gas controller (Agilent). After 4 hours of hypoxia ...
-
bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
-
bioRxiv - Microbiology 2024Quote: ... The amplified vectors were digested with Dpn1 and 1 µL of each (vector and promoter region insert) were transformed into ultracompetent XL-10 Gold cells (Agilent) for in vivo assembly ...
-
bioRxiv - Immunology 2024Quote: ... Serum samples were diluted 1:50 in PBS + 25% FBS and data were acquired with a BioTek Synergy H1 plate reader (Agilent). ELISA data were analyzed with GraphPad Prism (Dotmatics ...
-
bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
-
bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002, Agilent Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002 ...
-
bioRxiv - Cancer Biology 2024Quote: ... The LZK Q240S drug-resistant construct and K195M kinase-dead mutant (1) was introduced using a Site-Directed Mutagenesis Kit (Stratagene) (Primers listed in Supplementary table S2) ...
-
bioRxiv - Cell Biology 2024Quote: ... Fluorescence was monitored with excitation and emission filters set at 465 and 590 nm during gradual heating (1 K/min) from 25 °C to 95 °C in a MX3005P real-time PCR instrument (Stratagene). Data was evaluated using MXPro3005 software (Stratagene ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed 5 times with 1X PBST and incubated with horseradish peroxidase conjugated secondary anti-mouse or anti-rabbit IgG (1:5000, Cat # P0161 and P0448, respectively; DAKO) in 1X PBST for 1 h at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... the peptide arrays were incubated with a 1:1,000 dilution of an appropriate antibody peroxidase conjugate (anti-rabbit immunoglobulins HRP conjugate, DAKO, P0217) for one hour at 25°C ...
-
bioRxiv - Cell Biology 2024Quote: ... A 96-well flat bottom MaxiSorp immuno plate was coated with 1 µg/mL anti-vWF antibody (Dako rabbit polyclonal) in a sodium carbonate/bicarbonate buffer pH 9.5 for 1 h at room temperature prior to blocking with 3% w/v BSA in PBS-T for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... RPE1 cells (1×104) of the indicated genotypes were seeded in 96-well Seahorse XFe96 cell culture microplates (Agilent Technologies) for 24 h in eight replicate wells ...
-
bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
-
bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
-
bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
-
bioRxiv - Microbiology 2020Quote: The quality of purified libraries was analyzed on a Bioanalyzer High Sensitivity DNA chip (Agilent; see Figure 1 for an example) by following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...