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Citations for Agilent :
2201 - 2250 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: Treated and control cell lysates were subjected to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described24 ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial respiration and glycolysis were assessed using Seahorse extracellular flux analyzer (XFe96) and Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, USA). Cells were seeded in 96-well plates (2 × 104 cells/well) ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Cell Biology 2020Quote: His-tagged Ran in the pET28 vector (a gift from Dr. Günter Blobel) was transformed into BL21 (DE3) RIL-competent cells (Stratagene, St. Louis, MO). Expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were assessed for quality and quantification according to the Chromium Single Cell 3’ Reagent kit v3 user guide using the High Sensitivity D5000 ScreenTape (Agilent 5067-5592) and 2200 TapeStation Controller software (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit, 302107).
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bioRxiv - Cell Biology 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of NSPCs was measured using the Seahorse XF Cell Energy Phenotype Test Kit (Agilent, # 103325-100), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... slices were then incubated with anti-cleaved caspase 3 antibody or anti-Ki67 antibody (Cell Signalling) and further processed with secondary antibody (LSAB2 horseradish peroxidase kit; Dako, Copenhagen, Denmark).
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bioRxiv - Biochemistry 2021Quote: ... fluorescence of labeled and unstained control cells was quantified with an ACEA NovoCyte benchtop flow cytometer (Agilent Technologies, Santa Clara, CA, USA). After applying a cut-off of 7.5 × 105 to the forward scatter ...
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bioRxiv - Immunology 2019Quote: Individual subunits (Strep-Tactin and dead streptavidin-SpyCatcher or dead streptavidin and Strep-Tactin-SpyCatcherΔ) were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies # 230280) and refolded from inclusion bodies using a modified version of the protocol previously described by Howarth and Ting43 ...
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bioRxiv - Neuroscience 2022Quote: Co-transfection of pAAV-CAG-DIO-(hCAR)off-(GtACR2Kv2.1-MuGFP)on-W3SL with pDPI and pDPII plasmids (Grimm et al., 2003) into AAV293 cells (Agilent Technologies, CA, USA) preceded harvesting and iodixanol gradient purification (as described by (Zolotukhin et al. ...
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bioRxiv - Plant Biology 2022Quote: ... USA) equipped with a Cobalt cell (Teledyne Photon Machines) and connected to an Inductively Coupled Mass Spectrometer (model 8900, Agilent Technologies, USA). Analysis was done with the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 1 hour followed by vehicle or TGF-β (5 ng/ml) and 3000 cells were seeded into Seahorse assay microplates (Agilent Technologies). After 24h of incubation ...
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bioRxiv - Cell Biology 2022Quote: ... Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in MIN6 cells using an XF24 Analyzer (Seahorse Bioscience, MA, USA).
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Biophysics 2023Quote: ... The cells were fixed with 3% formalin 3 days after the transfection and analyzed with the ACEA Quanteon (Agilent NovoExpresss Version 1.5.0), as described (22 ...
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bioRxiv - Immunology 2023Quote: Strep-Tactin SpyCatcher and dead streptavidin (monovalent) or Strep-Tactin SpyCatcher and Strep-Tactin (tetravalent) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies #230280) were combined and refolded from inclusion bodies ...
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bioRxiv - Cell Biology 2023Quote: ... and Extra Cellular Acidification Rate (ECAR) values were normalized based on cell numbers per each well using Wave software (Agilent Technologies, Inc). Data analysis and visualization were performed using GraphPad Prism (Version 9.5.1 (528)).
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bioRxiv - Neuroscience 2024Quote: ... OCR and ECAR were measured as follows: cell culture media was removed and 80µl of XF Media (Agilent Technologies; Cat #: 103334-100) containing no pH buffers and supplemented with 5.5 mM glucose ...
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bioRxiv - Neuroscience 2024Quote: ... 10K HMC3 microglia per well were seeded in 80µl of a Agilent Seahorse XF96 Cell Culture Microplate (Agilent Technologies; Cat #: 101085-004) and incubated overnight ...
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bioRxiv - Systems Biology 2024Quote: Media to be used for cultivating cells for intracellular metal quantification with ICP-MS was through a PVDF membrane plate (Agilent 200931-100) into fresh deep-well (2mL ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Neuroscience 2024Quote: OCR assay: Cells were washed and incubated for 1 hour with 180 μL assay medium [in mmol/L: 5 glucose (Agilent #103577-100), 1 pyruvate (Agilent #103578-100) ...
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bioRxiv - Cell Biology 2024Quote: MitoSnap+ and MitoSnap-cells were purified by FACS and their oxygen consumption rates (OCR) were measured using a XF96 MitoStress Test (Seahorse Agilent, 103015-100). Activated CD8+ T cells were washed in RPMI 1640 without sodium bicarbonate ...
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bioRxiv - Cancer Biology 2024Quote: ... Drug was then washed out and 1x105 cells plated in 5 replicate wells for immediate analysis using the Mito Stress Test Kit (Agilent, 103015-100) according to the manufacturer’s instructions and using the following drug concentrations ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... UACC-62 and RPE cells was characterized by extracellular flux analysis using Agilent Seahorse XF96 Analyzer (Agilent Seahorse Bioscience, Santa Clara, CA, USA), as an indicator of the functional bioenergetic capacity of the mitochondria and overall cellular health in response to TRMT61B downregulation.
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bioRxiv - Genomics 2021Quote: Exome capture was carried out for six cell lines and 39 mixtures using SureSelect Human All Exon V6 (Agilent Technologies, Inc., CA, USA). To minimize duplicate reads in ultra-deep sequencing ...
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bioRxiv - Immunology 2021Quote: ... F4/80 (Serotec MCA497GA, 1:400), wide-spectrum keratin (CKWSS, which labels bile duct epithelium and hepatic progenitor cells (Dako #Z0622, 1:400)) or SMA (Dako #M0851 ...
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bioRxiv - Cell Biology 2021Quote: ... A P/O ratio of 2.75 has been validated for calculating the OXPHOS ATP production rate in cells (Romero N et al Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology). Thus ...
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bioRxiv - Microbiology 2020Quote: ... 2008 was filtered through 3 μm and sent to Bigelow Laboratory for Ocean Sciences (Boothbay Harbor, ME, USA) for cell sorting in a MoFlo™ Flow Cytometer (Dako-Cytomation, Denmark). Digestive vacuoles of heterotrophic protists were stained using the vital stain LysoTracker® (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Cancer Biology 2022Quote: Real time extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in cells were determined using the Seahorse Extracellular Flux (XFe-96) analyzer (Seahorse Bioscience, MA, USA). 1×104 cells were seeded per well of XF96 cell culture plates and incubated for 24 h to allow adherence ...
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bioRxiv - Molecular Biology 2019Quote: ... and extracellular acidification rate (ECAR) in HKC-8 cells were studied by using a Seahorse XF24 Extracellular Flux Analyzer (Agilent, Santa Clara, CA). Key parameters of mitochondrial function were assessed by directly measuring the OCR in HKC-8 cells transfected with 40 nM of either miR-9-5p or miR-NC and treated with 10 ng/ml TGF-β1 for 48h ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 1X with 200 mL warm ATP Assay Medium prepared per protocol (Agilent XF DMEM Medium pH 7.4 (103757-100); 10 mM XF Glucose ...
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bioRxiv - Immunology 2020Quote: ... Cells cultured on 100 mm plates were washed once with PBS and cell monolayers were irradiated with 100 mJ in a UV-Stratalinker 2400 (Stratagene, San Diego, CA) with the plate lid off or on to generate the mock control ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Cell Biology 2022Quote: ... 1.2 × 104 NIH3T3 cells per 100 μl of growth medium were transfected with DNAs or siRNAs as described above and seeded onto a Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) for 32 h ...
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bioRxiv - Microbiology 2022Quote: ... The reduction of resazurin by viable Vero cells was measured with 530 emission and 590 excitation settings on a BioTek Synergy HTX multimode reader (Agilent, Santa Clara, CA) with Gen5 version 3.10 acquisition software ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells (5 × 105 cells/ml) were pretreated for 1 hour in Seahorse Seahorse XF base medium supplemented with glutamine (103334-100, Agilent Technologies, Heverlee, Belgium) in a CO2-free incubator ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were harvested from the T-75 flasks and seeded into a Seahorse 96-well XF Cell Culture microplate (Agilent Seahorse Bioscience, CA, USA) in 80 µL of the culture medium at a density of 20,000/well ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Overexpjression of MTf was accomplished by stably transfecting SK-MEL-28 cells with human MTf cDNA or with the empty pCMV-Script® (Stratagene, CA, USA) vector alone14 ...