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Citations for Agilent :
1801 - 1850 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Oxygen consumption rate (OCR) was measured using a Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100). iMACs were resuspended in an RPMI1640 medium containing LPS (50 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... individual stained coverslips with cells were carefully transferred with tweezers to slides with prepared mounting medium (S3023, DAKO). Fluorescence detection of ORF1p and ORF2p was performed the day after the mounting medium dried using a ZEISS 700LSM confocal microscope with plan-apochromat 40x oil objective lens ...
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bioRxiv - Cancer Biology 2023Quote: Mitochondria respiration and real-time ATP production was assessed by Seahorse XF Cell Mito Stress Test Kit (Agilent) and Seahorse XF Real-Time ATP Rate Assay Kit (Agilent) ...
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bioRxiv - Cell Biology 2023Quote: ... the cell maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 1mM sodium pyruvate (Corning Cellgro) ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary VSMCs (2x104-3x104) were seeded onto to an Xfe96 poly-D-Lysine (PDL) Cell Culture Plate (Agilent) in 80uL of 5% FBS ...
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bioRxiv - Cell Biology 2023Quote: The oxygen consumption rate in cybrid cell lines was detected using an XFe24 extracellular flux analyzer (Seahorse Bioscience) as described59 with some modifications ...
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bioRxiv - Cell Biology 2024Quote: Myotubes were cultured in 96-well Seahorse XF cell culture microplates (#103015-100, Seahorse Bioscience, Agilent Technologies, US). Before running the assay ...
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bioRxiv - Cell Biology 2024Quote: Myotubes were cultured in 96-well Seahorse XF cell culture microplates (#103015-100, Seahorse Bioscience, Agilent Technologies, US). Before running the assay ...
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bioRxiv - Genomics 2024Quote: ... the pETM11-Sumo3-Tn5(E54K,L372P) plasmid was transformed into Escherichia coli BL21(DE3) codon + RIL cells (Stratagene). Cells were grown in TB-FB media (1.2 % Bacto-Tryptone ...
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bioRxiv - Cell Biology 2024Quote: ... the cells’ ATP production rate was analysed with the Seahorse XF Real- Time ATP Rate Assay kit (Agilent) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the plate containing the follicles was transferred to a live-cell imaging system (Agilent BioTek Cytation 5, USA) to capture images at 6-minute intervals during ovulation ...
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bioRxiv - Bioengineering 2024Quote: ... and the number of cells that were alive or dead were quantified using Gen 5 software (Agilent Technologies).
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bioRxiv - Cell Biology 2024Quote: ... and cells were washed twice with 200 µL of the respective assay medium for mito-stress test (Agilent Base Medium supplemented with 1mM pyruvate ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells (0.2 x 106/well) were counted and plated in XF RPMI medium (pH 7.4, Agilent 103576) containing 10mM glucose (103577 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated in 500 µL Seahorse XF Base Medium Minimal DMEM without Phenol Red (Agilent, 103335-100) 1 h at 37 °C in CO2 free-atmosphere ...
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bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
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bioRxiv - Biophysics 2021Quote: Room-temperature (RT) absorption spectra of cells were acquired on a Varian Cary 4000 UV–Vis-spectrophotometer (Agilent technologies) equipped with an integrating diffuse reflectance sphere (DRA-CA-50 ...
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bioRxiv - Biophysics 2021Quote: ... Mutations were introduced through site-directed mutagenesis (QuikChange II XL, with 10 XL Gold cells; Agilent Technologies, Kista, Sweden) and confirmed by sequencing at the Linköping University Core Facility.
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bioRxiv - Cell Biology 2020Quote: ... 2 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 0.5 μM rotenone + 0.5 μM antimycin from the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Data was analyzed by the Seahorse XF Cell Mito Stress Test Report Generator ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR products of positive clones were then cloned into competent cells using the StrataClone PCR Cloning Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... A specialised flow cell was employed in conjunction with an inline Agilent 1200 HPLC system (Agilent Technologies, Stockport, UK); sense and antisense AluSx1 RNA samples were injected onto a Shodex KW403-4F (Showa Denko America Inc. ...
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bioRxiv - Genomics 2020Quote: ... The ratio between cell-free DNA and high-molecular weight DNA (HMW) was calculated with the Prosize 3.0 software (Agilent). To this end ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial function was assessed in live cells using an XFp Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA, USA). After 24 hours silencing (siNeg vs siCFH ...
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bioRxiv - Biochemistry 2019Quote: ... encoding N-terminally 6xHis tagged MBP-MS2 (Gift from Nadège Liaud, UC-Berkeley) was transformed into BL21 Codon+ RIL chemically competent cells (Agilent). An overnight culture was used to inoculate 3L of ZYM-5052 auto-inducing media (Studier ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... FACS sorted MPs were plated on XF96 cell culture microplates coated with 10% Matrigel in warm assay medium (Agilent), the cell culture plate was centrifuged with 200 g for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Seahorse records were corrected for well-to-well differences in the cell numbers and analyzed using Wave software (Agilent). Leukemia cells (40,000 cells/well for cell lines ...
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bioRxiv - Cancer Biology 2020Quote: ... A549N cells were seeded in normal media in Seahorse xFP plates (Cat# 103022-100, Agilent Technologies, Santa Clara, CA) 24 h prior to conducting the assay ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2×107 HeLa cells were crosslinked on ice with 0.8 J/cm2 UV 254 nm in a UV Stratalinker 2400 (Stratagene), washed in PBS with 2 mM EDTA and 0.2 mM PMSF ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Cancer Biology 2020Quote: Untargeted metabolomics profiling of PCK1-overexpressing cells was performed using ultra-high-performance liquid chromatography (Agilent 1290 Infinity LC) coupled to with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Subsequently we strictly adhered to the Seahorse XF Cell Mito Stress Test Kit protocol (Agilent Technologies Cat# 103015-100). Cells were counted before and after the assay using a Celigo S Image cytometer (Nexcelom Biosciences ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... at 22.4µg/ml concentration per well to prepare Cell-Tak coated XF96 microplates according to the manufacturers guidelines (Agilent Technologies). Cells were seeded into the coated microplates immediately before the assay by centrifugation with 200g for 1min without brake ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 104 cells were seeded into each well of a V3 96-well plate (Agilent, Cat. # 101085-004) and cultured 24 h before measuring OCR ...
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bioRxiv - Cell Biology 2020Quote: ... The number of cells per well was directly incorporated into the Wave software (Seahorse Wave Controller 2.4 software, Agilent) to normalize OCR and ECAR values ...
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bioRxiv - Bioengineering 2020Quote: ... VO2max = 1.04×10−16 mol/s/cell was measured for hiPSC-Heps using the Seahorse XF24 cellular respirometer (Agilent) (see ...
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bioRxiv - Cell Biology 2021Quote: Oxygen consumption rates (OCR) from differentiated SVF cells were analyzed by the Seahorse XF24 Extracellular Flux Analyzer - (Agilent Technologies). Cells were seeded ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolated mouse cardiomyocytes were seeded at 2K per well in the Seahorse XF96 cell culture microplates (Agilent Technologies, 102416100) pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were overlaid with 175 μL of mitochondrial assay solution [33] supplemented with the Seahorse Plasma Membrane Permeabilizer (Agilent), 4 mM ADP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: iNeurons were replated on DIV 4 onto polyornithine/laminin coated in the Seahorse XF96 Cell Culture Microplate (Agilent Technologies) at a density of 50 000/well ...
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bioRxiv - Neuroscience 2019Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience) via changes in the oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2019Quote: ... harvested after 72 hours of culture and plated on 96-well XF cell culture microplates in XF assay medium (pH 7.4, both from Agilent) supplemented with D-glucose (20mM ...
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bioRxiv - Biochemistry 2021Quote: ... These data were then normalized to the corresponding DNA/protein/cell number in the Wave data analysis software (Agilent) and exported to Microsoft Excel.
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cryopreserved tumour samples or cultured cells using the Total RNA Isolation Micro kit (Agilent) and cDNA then synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... and centrifuged again to remove the supernatant.RNA was then extracted from the isolated disc cells using Absolutely RNA Nanoprep Kit (Stratagene), following manufacturer’s protocol ...