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Citations for Agilent :
2001 - 2050 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded at a density of 40,000 cells per well in a Seahorse 96-well plate (Seahorse XFe96, Agilent Technologies). Cells were incubated with 10nM GIP or vehicle for 3 days ...
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bioRxiv - Cancer Biology 2022Quote: ... Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were measured in cell lines using a Seahorse XFe96 Analyzer (Agilent).
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bioRxiv - Cell Biology 2022Quote: ... on a OCR and ECAR were measured using a Seahorse XF Cell Mito Stress Test kit (103015-100, Agilent Technologies) as described above.
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bioRxiv - Neuroscience 2022Quote: Endogenous OCR was measured in adherent SH-SY5Y cells using a Seahorse XF-24 Analyzer (Seahorse Bioscience, Billerica, MA, USA) following the manufacturer’s protocol with minor modifications (63) ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Neuroscience 2023Quote: ... Primary fibroblasts plated in 96-well assay plate at a density of 40,000 cells/well were cultured overnight and then equilibrated with Seahorse XF base medium (Agilent Technologies) supplemented with glucose ...
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bioRxiv - Biochemistry 2022Quote: ... The sensor plate was calibrated in the machine prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 analyser (Agilent). Following assays ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Immunology 2023Quote: ... and 1-2×105 T cells per well were attached in 5-8 replicates in Seahorse XF RPMI medium (Agilent) supplemented with 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously (71 ...
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bioRxiv - Cancer Biology 2023Quote: The oxygen consumption rate (OCR) of WT and TARBP1 KO cells was measured by using the XF96 extracellular flux analyzer (Agilent). 30000 cells were seeded onto one well of mini plates ...
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bioRxiv - Cell Biology 2023Quote: ... Vectors encoding the recombinant proteins were constructed as described in the previous section and introduced into the BL21-CondonPlus (DE3)-RIL competent cells (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... GEMs were used to generate barcoded cDNA libraries following the manufacturer’s protocol (Single Cell 3’ Reagent Kit v3.1, 10X Genomics) and quantified using the TapeStation High Sensitivity D5000 kit (Agilent, Germany). Subsequently ...
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Genomics 2023Quote: ... the size of the library of 2000 cells was measured using a High Sensitivity D1000 ScreenTape (Agilent Technologies, 5067-5584) on a TapeStation (Agilent Technologies ...
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bioRxiv - Pathology 2023Quote: ... and oxygen consumption rates (OCR, XF Cell Mito Stress Test) were performed with a Seahorse XF24 Extracellular Flux Analyzer and test kits (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The different instrumentation used corresponds to the two separate experimental batches (HySic Study I: A549 and NCI-H358 cell lines, H-FX-Agilent 1290 setup ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence in the plasmid used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: OCR and ECAR were measured in XF96 cell culture microplates using a Seahorse XFe96 Extracellular Flux Analyzer (Seahorse, Agilent Technologies). ISE6 cells were seeded at densities of 120 – 150,000 cells per well in complete L15C300 media41 and incubated at 34°C for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Agilent Seahorse XF Cell Mito Stress Test Kit was used to perform Mitostress test in the presence of 10mM glucose (Agilent) with addition of Oligomycin ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Immunology 2023Quote: 3.5×105 HIF-1α-deficient or WT BMDCs were plated in Seahorse XF96 Cell Culture Microplates (Agilent Technologies, #101085-004). Cells were kept in 180 μL of assay medium (supplemented with 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of live and dead cells in 30 µL was counted using a NovoCyte 2100YB Flow Cytometer (Agilent Technologies). Vincristine sulfate (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The sensor plate was calibrated prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 (Agilent). Following assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell Mito Stress Test was further combined with substrate pathway-specific inhibitors (etomoxir, UK5099, BPTES) (Agilent Technologies, Santa Clara, CA) to interrogate which of the three primary substrates (long chain fatty acids ...
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bioRxiv - Biochemistry 2023Quote: ... Successful mutation of isolated clones was verified by Sanger sequencing and clones were retransformed into BL21-CodonPlus (DE3)-RP competent cells (Agilent) for protein expression.
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2023Quote: ... non-targeted BMDCs or HIF-1α-deficient BMDCs were plated in Seahorse XF96 Cell Culture Microplates (Agilent Technologies, #101085-004) and stimulated with 10 mM of L-LA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Measurements of real-time of oxygen consumption rates (OCR) were determined in whole cells using a Seahorse XF96 extracellular flux analyser (Agilent) as described previously (78) ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from HEK293T cells as described above and RIN determined to be 10 for all samples (Bioanalyzer 6000, Agilent). Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell 3′ RNA-seq and single-cell ATAC-seq libraries were quantified on an Agilent Bioanalyzer with a high-sensitivity chip (Agilent), and Kapa DNA quantification kit for Illumina platforms (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Several metabolic parameters were assessed in sorted cDC1 using the XF Cell Mito Stress Test and Seahorse XFe96 Analyzer (Agilent). cDC1s were labeled with PE-XCR1 antibody for positive selection using magnet after pan-DC enrichment (Miltenyi) ...
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bioRxiv - Microbiology 2023Quote: ... cover slips containing cells were mounted using VITROCULD mounting media (Deltalab) and viewed using Lionheart LX Automated Microscope (Agilent BioTek) at 40X magnification ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.5 × 105 Bmal1::luciferase MAFs or Per2::luciferase PDA cells were seeded per well in 96-well black-sided imaging plates (101085-004, Agilent) pre-coated with 0.2% gelatin for 1 hour at 37°C ...
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bioRxiv - Physiology 2023Quote: ... The cells were transfected with 100 ng of NF-κB firefly luciferase reporter plasmid p(NF-κB)3-Luc (Stratagene) and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... the corresponding pAAV plasmid construct along with AAV helper plasmids encoding the structural elements were transfected using polyethylenimine (PEI) into the AAV-293 cell line (CVCL_6871, Agilent, USA). The day before transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... then plated at 3mL cell suspension/well into 44mm 24-deep-well round-bottom culture plates (Agilent Technologies #202061-300) using a Multidrop Combi (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
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bioRxiv - Microbiology 2023Quote: ... Membrane fluidity was determined by using an excitation wavelength of 350 nm and measuring fluorescence at 460 nm and 500 nm emission wavelengths on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The generalised polarisation (GP ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl were aliquoted into black 96 well plates in triplicate and fluorescence was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The excitation wavelength used was 488 nm and emission wavelength used was 530 nm.
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bioRxiv - Molecular Biology 2023Quote: ... The DNA obtained from cell cultures transfected with the RNP complex was amplified by nested PCR using Herculase II Fusion DNA Polymerase (Agilent). The first amplification was performed with primers IG36 and IG37 as follows ...
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bioRxiv - Microbiology 2023Quote: ... 500 or 750 μM DIP prior to transfer of 200 μL to the wells of a Seahorse XF96 V3 PS cell culture microplate (Agilent). The seahorse XF sensor cartridge (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were tested for oxygen consumption rate and ATP production following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Measurements were taken both at baseline prior to mitochondrial stress and following the addition of 2 μM FCCP uncoupling agent.