Labshake search
Citations for Agilent :
1851 - 1900 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2020Quote: ... the fragments were joined by Gibson assembly and used to transform chemically competent BL21-Gold(DE3) cells (Agilent Technologies). Individual clones were sequence-verified by Sanger sequencing (Microsynth AG) ...
-
bioRxiv - Molecular Biology 2019Quote: ... N≥50 worms (10 worms/ well) were transferred to the Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) in a total volume of 180 uL ...
-
bioRxiv - Cell Biology 2020Quote: Oxygen consumption rates (OCR) were measured in iBAs cells using a Seahorse XFe96 (Agilent Technologies, Santa Clara, CA, USA). Briefly ...
-
bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...
-
bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were blocked in 0.2% triton X-100 solution containing 1% BSA and 5% normal goat serum (DAKO, X0907) for 1 hour.
-
bioRxiv - Developmental Biology 2021Quote: ... at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat. no. 103575-100) supplemented with 10mM glucose (Agilent cat ...
-
bioRxiv - Cell Biology 2020Quote: Forty-thousand HUVECs representing each genotype (0RV, 1RV, and 2RV) were seeded on V7 cell culture plates (Seahorse Bioscience). Cells were either left untreated or treated with IFNɣ (50pg/mL ...
-
bioRxiv - Microbiology 2020Quote: ... The MUF production (fmol MUF cell-1 h-1) was measured with a Cary Eclipse spectrofluorometer (FL0902M009, Agilent Technologies) at 359 nm (excitation ...
-
bioRxiv - Microbiology 2020Quote: ... approximately 2×107 cells were crosslinked on ice with 0.8 J/cm2 UV 254 nm in a UV Stratalinker 2400 (Stratagene), washed in PBS with 2 mM EDTA and 0.2 mM PMSF ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3.104 cells were seeded into 96 well plates suitable for a 96-well metabolic analyser (Seahorse XF96, Agilent Technologies). 24 hours later and prior to the assay ...
-
bioRxiv - Immunology 2020Quote: ... A total of 1 × 106 PBMC was seeded in 24-well XF24 cell culture microplates (Agilent Technologies, Waldbronn, Germany), while four wells were kept free from cells as background correction ...
-
bioRxiv - Immunology 2021Quote: ... After 2h of AIM2 activation cells were processed per manufacturer’s directions and analyzed using the Agilent Seahorse Mito Stress Test kit (Agilent). For over-night stimulation with LPS cells were treated 2h after plating with 10 ng/mL LPS and incubated overnight at 37°C.
-
p97/VCP induces GLI1 to control XBP1-dependent endoplasmic reticulum stress transcriptional responsebioRxiv - Cancer Biology 2021Quote: ... Then in the dark (to protect the signal from light) the cells were incubated with 3,3’--diaminobenzidine (DAB, diaminobenzidine, EnVision System, Dako) for 10 min ...
-
bioRxiv - Microbiology 2022Quote: ... cooled down to room temperature for 20 minutes and used for the transformation of XL1 blue competent cells (Agilent). All identified mutations (Fig ...
-
bioRxiv - Cell Biology 2022Quote: ... the cell nuclei were stained with a 1:2000 dilution of SPY-DNA dye (Spirochrome) in antibody diluent (Dako). SPY-DNA 505 ...
-
bioRxiv - Genetics 2022Quote: The wild-type and mutant BRD-1-BRC-1 chimeras were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown at 37°C until OD600 0.6 and were induced by 0.2mM isopropyl-β-D-thiogalactoside in the presence of 100μM ZnCl2 at 37°C for 6 hrs ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and mounted on glass slide using mounting media (Dakocytomation Fluorescence Mounting Media, Dako, USA). The cells were examined using either a Nikon Eclipse 80i (Japan ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 0.5μM/0.5μM rotenone/antimycin A (all XFp Cell Mito Stress Test Kit, Agilent Technologies, 103010-100) according to manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were cloned into the StrataClone TA-cloning vector and transformed into StrataClone SoloPack competent cells according to the manufacturer’s protocol (StrataClone PCR Cloning Kit, Agilent), resulting in pFF-162 (SpoY) ...
-
bioRxiv - Cell Biology 2022Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used ...
-
bioRxiv - Cancer Biology 2023Quote: ... were seeded at a density of 5,000 cells/well in a 96-well plate (Part no.101085-004, Agilent) one day before the analysis ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and mounted on glass slide using mounting media (Dakocytomation Fluorescence Mounting Media, Dako, USA). The cells were examined using either a Nikon Eclipse 80i (Japan ...
-
bioRxiv - Microbiology 2023Quote: Vero TMPRSS2 cells were plated in 96-well tissue culture treated E-Plate VIEW plates (#300-601-020, Agilent) to be subconfluent at time of assay ...
-
bioRxiv - Pathology 2023Quote: Mitochondrial bioenergetics was determined by measuring oxygen consumption rate (OCR) of RPE cells with a Seahorse XFe96 Analyzer (Agilent, Santa Clara ...
-
bioRxiv - Immunology 2023Quote: ... surfaced stained cells were fixed and permeabilized according to the manufacturer’s instructions and analyzed immediately using the Novocyte Advanteon instrument (Agilent). The data were analyzed using FlowJo software ...
-
bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
-
bioRxiv - Biochemistry 2023Quote: ... COS1 cells were transfected with pAdTrack-CMV expression plasmids (Table S1) using the GeneJammer transfection reagent (#204130, Agilent Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... mESC or HCT116 cells were irradiated with 0.2 J/cm2 of 254 nm UV light in a Stratalinker 2400 (Stratagene). Cells were lysed in 1 ml of lysis buffer with Complete protease inhibitor (Roche) ...
-
bioRxiv - Cancer Biology 2023Quote: xCELLigence experiments were performed using the Real-Time Cell Analyzer (RTCA) Dual Plate (DP) instrument according to the manufacturer’s instructions (Agilent.com). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice and changed into Seahorse XF DMEM or RPMI medium (Agilent Technologies, 103680-100, 103681-100) containing 10 mM glucose ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed and incubated for 60 min in a CO2-free incubator in seahorse assay base media (Agilent) containing 25mM glucose (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... NEBuilder HiFi DNA Assembly Master Mix was used to assemble the inserts and backbones according to the manufacturer’s protocol and transformed into XL10-Gold competent cells (Agilent). Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research ...
-
bioRxiv - Cancer Biology 2023Quote: ... Thermo Fisher Scientific)-coated XF96 plates at 0.12×106 cells/well in 180 μL of XF base medium minimal DMEM media (#102353-100, Agilent) supplemented with 20 mM D-glucose (G6152 ...
-
bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
-
bioRxiv - Physiology 2023Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described11,24.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Raw 264.7 cells and BMDMs were seeded in Seahorse XF2 96 culture plates (102416-100, Agilent, Craven Arms, UK) 24 hours prior to treatment ...
-
bioRxiv - Cell Biology 2024Quote: Cellular OCR was measured using a Seahorse XF Cell Mito Stress Test and a Seahorse Bioanalyzer XFe96 (Agilent Technologies) according to the manufacturer’s standard protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent] ...
-
bioRxiv - Cell Biology 2024Quote: The oxygen consumption ratio (OCR) was determined using Seahorse XF Cell Mito Stress Test (Agilent, Santa Clara, CA, US). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... cell culture media was replaced with assay media (Seahorse XF DMEM medium pH 7.4, Cat. No. 103575-100, Agilent Technologies ...
-
bioRxiv - Biophysics 2023Quote: ... The PCR product was digested overnight with DpnI at 37 °C and transformed into XL1-Blue Supercompetent cells (Agilent) and the mutation was confirmed by sequencing (Eurofins Genomics) ...
-
bioRxiv - Cancer Biology 2023Quote: The mitochondrial function of MAFs was measured with a Seahorse XF Cell Mito Stress Test Kit (103015-100, Agilent) using a Seahorse XF96 Extracellular Flux Analyzer ...
-
bioRxiv - Immunology 2023Quote: ... 293FT cells were grown to ∼90% confluency in Corning Hyperflasks and co-transfected with 129 μg of pHELPER (Agilent), 238 μg of Rep-Cap plasmid encoding MG1.2 ...
-
bioRxiv - Cell Biology 2023Quote: HAECs were seeded at a density of 5,000 cells/well per on Seahorse XF24 tissue culture plates (Seahorse Bioscience). On the day of experiment ...
-
bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
-
bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
-
bioRxiv - Molecular Biology 2024Quote: HAECs were seeded at a density of 5,000 cells/well per on Seahorse XF24 tissue culture plates (Seahorse Bioscience). On the day of experiment ...
-
bioRxiv - Neuroscience 2024Quote: RNA samples of sorted Gsh2-lineage cells (eGFP+) from control and cKO embryos (E15.5) were submitted for quality control with the BioAnalyzer (Agilent) at the Genomics Core (KU Leuven) ...