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Citations for Agilent :
2101 - 2150 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... A mitochondrial stress test kit (Agilent, 103015-100) was used to monitor OCR where baseline measurements were made followed by sequential injection of ADP (4 mmol/L) ...
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bioRxiv - Cell Biology 2024Quote: ... A glycolysis stress test kit (Agilent, 103020-100) was used to monitor ECAR where baseline measurements were made followed by sequential injection of glucose (10 mmol/L) ...
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bioRxiv - Cell Biology 2024Quote: ... and AffinityScript QPCR cDNA Synthesis Kit (Agilent Technologies) with a set of specific primers (F ...
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bioRxiv - Molecular Biology 2024Quote: ... using the RNA ScreenTape Analysis Kit (Agilent Technologies).
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bioRxiv - Genomics 2020Quote: ... RNA was isolated using the RNeasy MinElute kit as per manufacturer’s instructions and was quantified using the Agilent Tapestation High Sensitivity RNA kit (Agilent 067-5576). RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and 75 base-pair single-end reads were sequenced using the Illumina NEXTseq 500 ...
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bioRxiv - Immunology 2022Quote: ... Human B Cell Kit (10X Genomics) and the quality and quantitation of the products were monitored using the High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina sequencer with Illumina adaptor primers found in the Chromium i7 Multiplex Kit.
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bioRxiv - Systems Biology 2020Quote: ... Total RNA was isolated from snap-frozen cells by using Quiagen AllPrep DNA/RNA Mini Kit (see simultaneous purification of DNA and RNA) and quantified using Agilent RNA 6000 Nano Kit (Agilent Technologies) on the Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2021Quote: ... IL17A and IL23 using Affinity QPCR cDNA Synthesis kit and Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix as per kit instructions (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2021Quote: The bioenergetic profile of BMDMs were assessed by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using a XF cell Mito Stress Test kit and XF glycolysis stress kit (Agilent Technologies) as per manufacturer’s instructions using a Seahorse XF extracellular flux analyzer (Agilent technologies ...
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bioRxiv - Microbiology 2020Quote: ... The library concentrations were measured using the Qubit™ dsDNA HS Assay Kit and the library insert size determined by the Agilent DNA 1000 Kit in a 2100 Electrophoresis Bioanalyser Instrument (Agilent, USA).
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bioRxiv - Immunology 2020Quote: ... RNA and cDNA quantity and quality were assessed using the Agilent RNA 6000 Nano Kit and Agilent RNA 6000 Pico Kit according to the manufacture’s protocol (Agilent Technologies, USA). Labelled cDNA were hybridised on a MouseWG-6 v2.0 Expression BeadChip (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... as described previously (67) by using the Seahorse XFp Cell Mito Stress Test Kit and XF glycolysis stress test kit (Agilent Technologies). OCR and ECAR were measured under basal conditions and in response to several metabolic drugs provided in the kit ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were quantified using a Qubit dsDNA HS DNA Kit (Thermo Fischer Scientific) and visualized on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced in a HiSeq2500 (Illumina Inc. ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial respiration and glycolysis were determined by measuring OCRs and ECARs in the extracellular space with a Seahorse XF96 Extracellular Flux Analyzer with the XF Cell Mito Stress Test Kit and XF Glycolysis Stress Test Kit (Seahorse Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified with Qubit dsDNA HS Assay kit and visualized with BioAnalyzer High Sensitivity DNA Analysis kit (Agilent 5067-4626) and 2100 BioAnalyzer Instrument (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was stored at −80°C and RNA quality was assessed by Bioanalyzer (Agilent 2100 Bioanalyzer, RNA 6000 Nano Kit for cells, RNA 6000 Pico Kit for EVs, Agilent Technologies).
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bioRxiv - Microbiology 2023Quote: ... Specific mutant constructs were subcloned from the mutants using appropriate restriction digestion sites or using the site-directed mutagenesis kit (Quick Change II Site directed mutagenesis kit, Agilent Technologies) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: Glycolysis and Mitochondrial stress tests were performed on confluent HUVECs according to the manufacturer’s kits and protocols (Agilent Seahorse XF glycolysis stress test kit, Agilent Technologies, Inc.). 1 μM Oligomycin was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 mM glucose (for the Cell Mito Stress Test Kit) or 1 mM glutamine (for the Glycolysis Stress Test Kit) was used as the assay medium (103681-100; Agilent Technologies). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... the quality of the libraries was assessed using Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer 2100 DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq 6000 with the sequencing configurations recommended by 10X Genomics ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Biophysics 2021Quote: ... Site-directed mutagenesis was performed using custom-designed primers (Eurofins Genomics) and regular PCR with PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies). Plasmids were linearized using EcoRI (for mASIC1a ...
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bioRxiv - Biochemistry 2021Quote: Impβ charge reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Impβ mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
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bioRxiv - Biochemistry 2021Quote: ... and Rev charge-reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Rev mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
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bioRxiv - Genomics 2019Quote: ... fragments with UMIs were generated using 200 ng starting material (purified by ethanol precipitation) in 2 cycles of PCR with Herculase II Fusion DNA Polymerase (Agilent Technologies) using an equimolar mixture of P023poolseqNN-primers (1 mM final concentration ...
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bioRxiv - Biochemistry 2019Quote: The genomic DNA template was amplified by the polymerase chain reaction (PCR) using Pfu Ultra High-Fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of genomic DNA (~12 ng) ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies).
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bioRxiv - Systems Biology 2021Quote: ... 607 steady-state LC and 208 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
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bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
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bioRxiv - Systems Biology 2021Quote: ... 700 steady-state LC and ~300 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
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bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies). 9-12 libraries were sequenced as a multiplex on one Illumina HiSeq 2000 lane using 125bp paired-end read length.
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bioRxiv - Cancer Biology 2020Quote: ... QRT-PCR was performed in triplicate including a non-template control using the Mx3000P system (Agilent Technologies, Inc., Santa Clara, CA). Oligos (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Bioengineering 2021Quote: The integrated sgRNA library was PCR amplified and separately barcoded for each collected population with Herculase II Fusion DNA Polymerase (Agilent 600677). Samples were then pooled and sequenced on an Illumina NextSeq flow cell with aligned read counts as follows for each screen:
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
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bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the three PCR reactions were then combined and analyzed for size on a High Sensitivity DNA chip (Agilent Technologies) and sequenced as SE100 ...
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bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
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bioRxiv - Microbiology 2020Quote: ... Abundances of bacterial and archaeal amoA genes and bacterial 16S rRNA genes were determined by quantitative PCR using a Mx3000P instrument (Agilent, CA). Primer combinations were AmoA-1F/ AmoA2R (Rotthauwe et al. ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification verification and library validation was done using a TapeStation electrophoresis system and D1000 ScreenTape reagents (Agilent, Santa Clara, California). Libraries were quantified using a Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... cDNA were amplified by 10 PCR cycles and purified before assessing their quality on the bioanalyzer (2100 high sensitivity DNA assay, Agilent Technologies). 150 pg of cDNA were used as input to prepare the libraries using the Nextera XT DNA kit (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: The full-length Fe-S cluster assembly protein SufB from the Mycobacterium tuberculosis H37Rv strain (Mtu FL-SufB) and its isolated intein (Mtu-SufB-I) genes were PCR-amplified using Pfu Ultra High-Fidelity DNA Polymerase (Agilent Technologies) from heat-killed Mtu genomic DNA ...
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bioRxiv - Genetics 2021Quote: ... All PCR amplification steps were performed using the high-fidelity Herculase II Fusion DNA Polymerase (Agilent, Santa Clara, CA, United States), and assembly of the recombinant vector was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies) and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed on 1:10 diluted cDNA using either SYBR-containing Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies) and gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA 7500 kit (Agilent Technologies).
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bioRxiv - Microbiology 2022Quote: ... the gentamicin resistance marker (PflaB-aaC1) was amplified from pMH105 (20) by PCR and then TA-cloned into pSC-A-amp/kan (Agilent Technologies). Following confirmation of the TA clones ...
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bioRxiv - Neuroscience 2023Quote: ... All point mutations of plasmids were generated by Quick Change PCR mutagenesis using Pfu Ultra polymerase (Agilent Technologies, Santa Clara, CA).