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Citations for Agilent :
2351 - 2400 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of each PCR product was subjected to Chip-based capillary electrophoresis with the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and the proportional amount of each fragment to the whole yield of splicing products was calculated ...
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bioRxiv - Microbiology 2020Quote: ... Touch-down method of PCR was used for increased specificity of primer amplification in a Surecycler 8800 (Agilent Technologies, Santa Clara, CA). The upper limit of annealing temperature was set at 65 °C and the lower limit at 53 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μL of the cDNA was used as template with the Thermo Scientific DyNAmo Flash SYBR Green PCR Master Mix in a Mx3005P QPCR System (Agilent Technologies, USA). A 2 step qPCR protocol was used ...
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bioRxiv - Microbiology 2020Quote: ... Lens was introduced to the leader region of the MC-II through MC-VI and MC-V-dotO arrays in our donor plasmids by quickchange PCR with Pfu Turbo Polymerase (Agilent #600250-52). boxA(−58 ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cancer Biology 2022Quote: ... by polymerase chain reaction (PCR). Ki-67 (Catalog no. M7240) and CD4 (Catalog no. M731029-2) antibodies were purchased from Dako (CA, USA). FOXO3a (Catalog no ...
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bioRxiv - Immunology 2019Quote: ... qRT-PCR reactions were made for selected genes using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies) as previously described.
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bioRxiv - Immunology 2019Quote: ... qRT-PCR reactions were made for selected genes using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies) as previously described.
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bioRxiv - Cancer Biology 2021Quote: ... PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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bioRxiv - Molecular Biology 2022Quote: ... The purity and integrity of the PCR products were analysed on an Agilent 2100 Bioanalyzer following manufacturer recommendations for high sensitivity DNA chips (Agilent 5067-4626). Sequencing libraries were prepared using Nextera DNA Library Prep Kit (Illumina FC-131-1024 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified mRNA was transferred to a clean nuclease-free PCR tube before the yield and size distribution was assessed using an RNA Pico Chip (Agilent Technologies, Inc.).
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bioRxiv - Plant Biology 2024Quote: Quantitative PCR (qPCR) was carried out in a 25 μL reaction volume using the Brilliant II SYBR Green PCR Master Mix (Stratagene, Agilent technologies). Primer concentrations were set at 0.2 μM ...
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bioRxiv - Immunology 2023Quote: ... The analysis of the PCR products was performed using the LabChip electrophoretic Agilent 2100 Bioanalyzer microchip electrophoresis system (Agilent Technologies, Wokingham, UK). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... All UTX isoforms and mutants were generated by PCR cloning into the pDONR221 vector or by site-directed mutagenesis using the QuikChange strategy (Agilent, CA, USA). Inserts were transferred to the pCDNA5_FRT_TO_N-GFP destination vector using GATEWAY cloning according to instructions of the manufacturer (Life technologies ...
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bioRxiv - Systems Biology 2023Quote: ... 7 cycles were used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System) and DNA 7500 (Agilent 2100 Bioanalyzer) ...
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bioRxiv - Developmental Biology 2024Quote: ... The CrxE1/CAG::OC1 element was cloned using polymerase chain reaction (PCR) with the high fidelity Herculase II Fusion DNA Polymerase (Agilent cat# 600675) to add “Sal1” sites to the 261bp enhancer sequence ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the supernatant was removed before exposing the plate to a temperature gradient for 3 minutes in a PCR machine (Agilent SureCycler 8800), followed by 3 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (KapaBiosystems; Cat#: KR0405) and library profiles were assessed using the DNA High Sensitivity HS Kit (Agilent; Cat#: 5067-4626) on an Agilent Bioanalyzer 2100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries fragment size distribution and concentration was assessed using Qubit dsDNA HS Assay Kit (Thermo Fischer Scientific, Q32851) and Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626) on a bioanalyzer ...
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bioRxiv - Cancer Biology 2024Quote: ... PolyA+ stranded RNA library was prepared using the Illumina Stranded mRNA Prep kit and quality was assessed by using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). RNA-seq libraries have been pooled equimolarly and sequenced using NovaSeq6000 (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... The electrical characteristics of the brain phantom were determined with a VNA equipped with a dielectric probe kit (85070E kit, Agilent Technologies, Santa Clara, CA) and were representative of the averaged human brain (er = 66.34 and σ = 0.49 S m−1) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit, Agilent, Santa Clara, CA, United States). Libraries were subsequently prepared and sequenced at Psomagen ...
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bioRxiv - Genomics 2024Quote: ... RNA quality was assessed using an Agilent 2100 Bioanalyzer with an RNA 6000 Nano Kit or RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA). ERCC ExFold RNA Spike-In Mixes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... IMAGE:6292760)) cloned in the pOTB7 vector (IRAUp969D10104D, RZPD Imagenes, Germany) served as template for site-directed PCR-mutagenesis (QuickChange, Stratagene, La Jolla, CA) according to the manufacturer’s instructions and as described in [52] and using the primer pairs (10 nmol ...
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bioRxiv - Physiology 2019Quote: ... amplified products were concentrated using a solid-phase reversible immobilization method for the purification of PCR products and quantified by electrophoresis using an (Agilent, Santa Clara, CA) 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2019Quote: ... Site-directed mutagenesis of the spaO gene was performed according to the QuikChange PCR site-directed mutagenesis protocol (Agilent, Santa Clara, CA, USA). All primers used in this study can be found in Table S5.
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments carrying both adapters after sodium bisulfite conversion were enriched by 8 cycles of PCR using the Pfu Turbo Cx Hotstart DNA Polymerase (Agilent, Santa Clara, CA). Primer dimers were subsequently removed using two rounds of purification using AMPure beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... The anc-1 3’UTR RNAi construct was generated by PCR amplifying part of the 3’UTR-region of the anc-1 transcript using Paq5000™ DNA Polymerase (Agilent Technologies, 600682). The primer sequences are detailed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... Fragment size was determined by loading 1 ng/μl cleaned up PCR product on the Fragment Analyzer System (Agilent Technologies, Santa Clara, USA) using the qualitative DNA Kit dsDNA Reagent 35-5000bp (Cat No ...
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bioRxiv - Microbiology 2020Quote: ... Full-length Vero-PVR1 and Vero-PVR2 cDNA was amplified with the Easy-A High Fidelity PCR Cloning Enzyme (Agilent Technologies, CA, USA), and cloned into T-vector pMD20 (Takara Bio Inc.) ...
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Fusion constructs and large deletions were generated by overhang extension PCR [79] using primers from IDT (IDT, Coralville, IA) and cloned Pfu DNA polymerase (Stratagene, La Jolla, CA). Point mutations and small deletions or insertions were generated using QuickChange [80] with Turbo Pfu DNA polymerase (Stratagene ...
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bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies, Waldbronn, Germany) which was used a PCR template for site-directed mutagenesis (A279T ...
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bioRxiv - Pathology 2020Quote: ... beads were collected for 2 min on a magnet and then resuspended in 25 µL PCR master mix containing PCR1 primers and Herculase-II (Agilent, Santa Clara CA). PCR cycling was as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... The locus-specific HDR donors were generated by PCR amplification of the MACHETE bicistronic cassette using a high-fidelity DNA polymerase (Herculase II, Agilent or Q5, NEB). PCR fragments were column purified (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... A total of 13 cycles was then used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System). Each type of library was pooled and sequenced using Illumina NovaSeq6000 System on SP flowcells [61] ...
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bioRxiv - Genomics 2023Quote: ... The PCR products obtained were purified (AMPure XP system) and library quality was assessed on an Agilent Bioanalyzer 2100 system (Agilent Technologies, United States). Library preparations of RNA were sequenced on the Illumina NovaSeq 6000 platform (Illumina Inc ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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bioRxiv - Microbiology 2024Quote: ... Quality assessment and quantification of the PCR products were respectively performed using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, CA, USA) and a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... and examined with the 2100 Bioanalyzer (RNA6000 Pico Kit, Agilent, cat.no ...
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bioRxiv - Cancer Biology 2021Quote: ... Quick Change XL Site-Directed Mutagenesis Kit (200516, Agilent Technologies) was used to construct ZHX2 mutants ...
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bioRxiv - Cancer Biology 2021Quote: ... and High Sensitivity DNA Kit (Agilent Technologies, Cat # 5067-4626). Generated DNA fragments were quantified with Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... capillary electrophoresis instrument and the CRISPR Discovery Gel Kit (Agilent). To verify that the E-box was successfully replaced by the LexO site ...
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bioRxiv - Cell Biology 2020Quote: ... Both vectors were edited using the QuickChange® kit (Stratagene) to generate constructs encoding PICK1 coding variants ...
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bioRxiv - Genetics 2021Quote: Site-directed mutagenesis was performed with QuikChange II kit (Agilent), using manufacturer recommended procedures.
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bioRxiv - Developmental Biology 2021Quote: ... Quality was confirmed using Agilent RNA 6000 Nano kit (Agilent) on the 2100 Bioanalyzer Instrument (Agilent ...
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bioRxiv - Genomics 2020Quote: ... DNA libraries were prepared by SureSelectXT Library Prep Kit (Agilent), hybridized to the appropriate capture panel ...
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bioRxiv - Cancer Biology 2022Quote: ... were sheared by the Sure Select Enzymatic Fragmentation kit (Agilent Technologies Inc. ...
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bioRxiv - Physiology 2022Quote: ... using an Agilent High Sensitivity DNA Kit (Agilent Technologies, France). Libraries were prepared from 0.15 ng cDNA using the Nextera XT Illumina library preparation kit (Illumina ...