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Citations for Agilent :
1901 - 1950 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... qRT-PCR was run on a Roche Lightcycler 480 using the Brilliant II SYBR Green qPCR Master Mix (Stratagene, 600804) using the following primers ...
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bioRxiv - Genomics 2019Quote: ... Approximately one-third of the library was amplified over three 70 μl PCR reactions using 5 μl of template each and Herculase II Fusion DNA Polymerase (Agilent). The products were MinElute purified ...
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bioRxiv - Neuroscience 2019Quote: ... All GFP-Parkin variants were generated using PCR mutagenesis on the GFP-Parkin WT plasmid (addgene#45875) according to the manufacturer’s protocol (Agilent Technologies). Constructs were verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2019Quote: ... PCR was carried out in a 20 μl reaction volume containing 10 μl Brilliant III SYBR Green Master Mix (Agilent), 500 nM of primers ...
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bioRxiv - Microbiology 2019Quote: ... The relative expression levels of targeted genes were measured by qRT PCR using Brilliant III ultra-fast SYBR green QPCR mix (Stratagene) in an Applied Biosystems 7500 Real-Time PCR system ...
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bioRxiv - Neuroscience 2019Quote: ... the mouse cDNA sequence for Ntrk2 (GenBank accession number X17647, nucleotides 188-722) was amplified by PCR and cloned into pBluscript II KS (-) plasmid (Stratagene). Antisense probes were synthesized with T3 RNA polymerase (Promega #2083 ...
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bioRxiv - Developmental Biology 2021Quote: ... a BirA-2A-Cherry-SV40pA-FRT-Kan-FRT cassette was PCR-amplified using Herculase II fusion DNA polymerase (Agilent Technologies) and recombined into the first coding exon of tcf21 within the DKEYP 79F12 BAC clone as previously described (Weinberger ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... qRT–PCR reactions were performed in duplicate using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) on a C1000 Touch™ thermos Cycler CFX96™ Real-Time System (BioRAD ...
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bioRxiv - Bioengineering 2021Quote: ... All PCR reactions for cloning and amplification of sequencing templates were performed using Herculase II Fusion DNA Polymerase (Agilent Technologies), and using GoTaq (Promega ...
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bioRxiv - Cell Biology 2021Quote: PCR amplification of inserted sgRNAs from the genomic DNA was done with the Herculase II Fusion DNA Polymerase (Agilent Technologies) using the primers oMCB-1562 and oMCB-1563 ...
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bioRxiv - Microbiology 2020Quote: ... the absence of DNA contamination was conformed using by PCR and the quality and quantity of the RNA samples was established using a 2100 Bioanalyzer (Agilent). Samples were sent for 150 bp paired-end sequencing via an Illumina platform and bioinformatic analysis ...
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bioRxiv - Cell Biology 2021Quote: Expression vectors were produced either by homologous recombination with PCR-amplified or synthetic genes and amplified backbones or site directed mutagenesis (QuikChange, Agilent). The wild type (Kumar et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was done using the Brilliant III Ultra-Fast SYBR® Green Master mix (Agilent Technologies, Santa Clara, CA) in an AriaMx (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: A library of IpaC mutants with missense mutations in the coiled-coil domain and flanking regions was generated using error-prone PCR with the GeneMorphII (Agilent) domain mutagenesis kit ...
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bioRxiv - Systems Biology 2020Quote: ... 3.5 μl/well was then used as template for 2nd PCR in a 50 μl reaction together with 0.5 μl Herculase II fusion DNA polymerase (Agilent Technologies), 10 μl 5* Herculase II reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Differentially labeled (Cy5–IgA-; Cy3–IgA+) half hairpin amplicons were PCR amplified and allowed to undergo competitive hybridization onto microarrays (Agilent). Candidate negative regulators were identified as genes that are enriched in the IgA+ population at least 2-fold over the IgA-population ...
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bioRxiv - Biophysics 2022Quote: All of mutations in this study were made by using overlap-extension polymerase chain reaction (PCR) with Pfu polymerase (Stratagene) on the template of the mbr5 splice variant of mslo1 (Uniprot ID ...
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bioRxiv - Microbiology 2021Quote: ... a ∼700 bp region in the S gene containing the P681H mutation and a ∼300 bp NSP3 covering the 3675-3677 deletion were amplified by PCR using Pfu UltraII (Agilent) using the following primers:
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bioRxiv - Molecular Biology 2020Quote: ... Converted libraries were enriched by 10 cycles of PCR with the following reaction composition: 1 μl Pfu TurboCx Hotstart DNA polymerase (Stratagene), 5 μl PfuTurbo Cx reaction buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with 15 PCR cycles used for the final amplification step and passed through quality control using a 2100 Bioanalyzer (Agilent). Both quality control and sequencing was carried out at Babraham Institute Next Generation Sequencing facility ...
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bioRxiv - Biochemistry 2020Quote: Mutants were prepared by QuikChange® site-directed mutagenesis using the Pfu-Turbo Hotstart PCR Master Mix (Agilent, Waldbronn, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Quality and quantity of PCR products were determined by using an Agilent 4150 TapeStation with High Sensitivity D5000 ScreenTape (Agilent). Final libraries were indexed using one of the 16 indexes for Illumina sequencing ...
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bioRxiv - Genetics 2022Quote: ... A ∼400bp region around the expected Cas9 cut site in exon1 of EXOSC2 was amplified by PCR using PfuTurbo DNA polymerase (Agilent), according to the manufacturer’s instructions using primers ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA sequence libraries were prepared from genomic DNA by two rounds of PCR using the Herculase II Fusion DNA Polymerase (Agilent). sgRNA sequences were amplified by the primers oMCB1562 and oMCB1563 and then indexed using the Illumina TruSeq LT adaptor sequences (AD002 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each sample pool was barcoded with a specific 10 bp-sequence attached at one side of the amplicons through PCR (7 cycles) and cleaned using AMPure beads (Agilent) at x0.7 ratio ...
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bioRxiv - Neuroscience 2019Quote: ... or Linker 2 (between Kv3.1 and miRFP670) were generated either by overlap extension PCR or using site-directed mutagenesis (QuickChange, Agilent, USA). Plasmid pPuro-CAG-CheRiff-IRES-NLS-mTagBFP2 encoding the optogenetic activating opsin CheRiff was made by subcloning CheRiff ...
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bioRxiv - Cancer Biology 2020Quote: ... The quality and concentration of the PCR products was determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies). To generate the NGS libraries ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT N-terminal point mutants were generated from pCR4 TOPO NOCT (Open Biosystems) and Quikchange site directed mutagenesis PCR (Agilent) to generate NOCT(1-431 ...
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bioRxiv - Immunology 2019Quote: ... The qRT-PCR reaction was made using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... ChIP and Input DNA were used to amplify target sequences of interest using PerfeCTa SYBR green PCR mix (Quanta Biosciences) and a MX3000 (Agilent) instrument ...
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bioRxiv - Microbiology 2019Quote: ... In case of ELF1α amplification was carried out in a 15 μL reaction volume containing 7.5 μL of 2X Brilliant III Ultra-Fast SYBR® Green QRT-PCR Master Mix (Stratagene), 0.75 μL of each forward and reverse primers (0.5 μM) ...
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bioRxiv - Cell Biology 2020Quote: The mCherry-TRAK1 deletion mutant mCherry-TRAK1Δ was obtained by inserting a stop codon after amino acid 635 of the mCherry-TRAK1 encoding nucleotide sequence by means of a PCR-based mutagenesis (Agilent Technologies ...
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bioRxiv - Genetics 2020Quote: ... The previously reported RALY intronic duplication 23 was genotyped by size differentiation of PCR products on a Fragment Analyzer (Agilent). Five PCR-assays (ventral promoter assays 1 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR purification step was performed followed by assessment of size distribution using an Agilent high-sensitivity DNA chip (Agilent technologies) and expression of selected genes was also examined at this stage using Taqman real-time qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... The Tah1 mutation in the MEEVD binding site was achieved by PCR-based site-directed mutagenesis (Quick-Change method, Stratagene) using suitable primer sequences with the desired mutation ...
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bioRxiv - Zoology 2021Quote: ... Forty cycles of PCR were carried out on a Stratagene Mx3005P QPCR system using MXPro MX3005 v3 software (Stratagene, UK). Cycle threshold (Ct ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape ...
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bioRxiv - Developmental Biology 2021Quote: ... Half of the cDNA was amplified for 17 PCR cycles and a 1:4 dilution of the resulting library was assessed by Agilent Bioanalyzer High Sensitivity DNA kit ...
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bioRxiv - Cell Biology 2020Quote: ... 6 PCR reactions including two biological replicates and 3 technical replicates per gene were performed using Brilliant SYBR master mix (Agilent), running cycles of 10 minutes at 95°C ...
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bioRxiv - Microbiology 2021Quote: ... three independent PCR reactions were carried out in a final volume of 50 µl with Herculase II fusion DNA polymerase (Agilent), then pooled and confirmed on gel electrophoresis for both fungi and bacteria ...
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bioRxiv - Molecular Biology 2022Quote: ... Phosphorylation site mutations and gRNA sequences were introduced into vectors by PCR amplification with mutagenic primers (Table 3) with Phusion (Thermo) or PfuUltra II (Stratagene) polymerase ...
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bioRxiv - Immunology 2022Quote: ... 60ng of DNA was used for PCR mix reaction prepared according to the manufacturer protocol (Herculase II Fusion DNA Polymerase, Agilent). The following Cre primers were used to carry out the PCR amplification ...
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bioRxiv - Microbiology 2022Quote: ... a pool of sgRNA-encoding inserts was generated by PCR amplification with primers oJMP697 and oJMP698 from a 78-nt custom oligonucleotide library (2020-OL-J, Agilent) with the following conditions per 500 µl reaction ...
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bioRxiv - Genomics 2022Quote: ... Hi-C material was then amplified from the beads with 7-9 cycles of PCR using Herculase II Fusion DNA polymerase (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Guide sequence libraries were prepared from genomic DNA by two rounds of PCR using the Herculase II Fusion DNA Polymerase (Agilent). First ...
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bioRxiv - Plant Biology 2024Quote: ... The synthesized cDNA was subsequently used as template for quantitative real time PCR based analysis of the expression levels of the target genes using Brilliant III Ultra-Fast SYBR QPCR MM (Agilent) in conjunction with Rotor-Gene® Q (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of the amplified barcode product in each PCR was measured using D1000 screentape reagents (5067-5582, Agilent Technologies) and the Tapestation instrument (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR reactions were performed in duplicate using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) on a C1000 Touch™ thermos Cycler using the CFX96™ Real-Time System (BioRAD ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of the genomic DNA was adjusted to 200 ng/µL before it was PCR amplified using Herculase II (Agilent). Approximately 400 ng purified PCR product (PCR purification kit ...
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...