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Citations for Agilent :
1951 - 2000 of 2011 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... F4/80 (Serotec MCA497GA, 1:400), wide-spectrum keratin (CKWSS, which labels bile duct epithelium and hepatic progenitor cells (Dako #Z0622, 1:400)) or SMA (Dako #M0851 ...
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bioRxiv - Cell Biology 2021Quote: ... A P/O ratio of 2.75 has been validated for calculating the OXPHOS ATP production rate in cells (Romero N et al Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology). Thus ...
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bioRxiv - Microbiology 2020Quote: ... 2008 was filtered through 3 μm and sent to Bigelow Laboratory for Ocean Sciences (Boothbay Harbor, ME, USA) for cell sorting in a MoFlo™ Flow Cytometer (Dako-Cytomation, Denmark). Digestive vacuoles of heterotrophic protists were stained using the vital stain LysoTracker® (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Cancer Biology 2022Quote: Real time extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in cells were determined using the Seahorse Extracellular Flux (XFe-96) analyzer (Seahorse Bioscience, MA, USA). 1×104 cells were seeded per well of XF96 cell culture plates and incubated for 24 h to allow adherence ...
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bioRxiv - Molecular Biology 2019Quote: ... and extracellular acidification rate (ECAR) in HKC-8 cells were studied by using a Seahorse XF24 Extracellular Flux Analyzer (Agilent, Santa Clara, CA). Key parameters of mitochondrial function were assessed by directly measuring the OCR in HKC-8 cells transfected with 40 nM of either miR-9-5p or miR-NC and treated with 10 ng/ml TGF-β1 for 48h ...
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bioRxiv - Microbiology 2019Quote: ... The reaction products for the total RNA of the human cell line KMM-1 were analysed by microfluidics-based automated electrophoresis with the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 100 µl of 0.5% BSA in PBS and incubated with mouse anti-human CD31 (Agilent Dako, M0823, clone JC70A) antibody-coated magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... The processed T cell samples were pooled and quantified relatively utilizing capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS, Agilent Technologies, Waldbronn, Germany) with analysis performed by Human Metabolome Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 1X with 200 mL warm ATP Assay Medium prepared per protocol (Agilent XF DMEM Medium pH 7.4 (103757-100); 10 mM XF Glucose ...
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bioRxiv - Immunology 2020Quote: ... Cells cultured on 100 mm plates were washed once with PBS and cell monolayers were irradiated with 100 mJ in a UV-Stratalinker 2400 (Stratagene, San Diego, CA) with the plate lid off or on to generate the mock control ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Cell Biology 2022Quote: ... 1.2 × 104 NIH3T3 cells per 100 μl of growth medium were transfected with DNAs or siRNAs as described above and seeded onto a Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) for 32 h ...
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bioRxiv - Microbiology 2022Quote: ... The reduction of resazurin by viable Vero cells was measured with 530 emission and 590 excitation settings on a BioTek Synergy HTX multimode reader (Agilent, Santa Clara, CA) with Gen5 version 3.10 acquisition software ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells (5 × 105 cells/ml) were pretreated for 1 hour in Seahorse Seahorse XF base medium supplemented with glutamine (103334-100, Agilent Technologies, Heverlee, Belgium) in a CO2-free incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Systems Biology 2023Quote: ... of GLUT4-HA-L6 cells myotubes was measured using Seahorse XFp miniplates and a Seahorse XF HS Mini Analyzer (Seahorse Bioscience, Copenhagen, Denmark) as previously described84 ...
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bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.25 × 105 KCL22 NTC and Ptbp2 KO cells were seeded in each well in 180μl of XF RPMI Assay Medium (Seahorse Bioscience, Cat. #103576–000) supplemented either with 2mM L-glutamine (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were harvested from the T-75 flasks and seeded into a Seahorse 96-well XF Cell Culture microplate (Agilent Seahorse Bioscience, CA, USA) in 80 µL of the culture medium at a density of 20,000/well ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Overexpjression of MTf was accomplished by stably transfecting SK-MEL-28 cells with human MTf cDNA or with the empty pCMV-Script® (Stratagene, CA, USA) vector alone14 ...
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bioRxiv - Cancer Biology 2023Quote: The Extra Cellular Acidification Rate (ECAR) of melanoma cells were determined using the Seahorse XF extracellular flux analyzer (Agilent, Santa Clara, CA, USA). The cells were plated ...
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bioRxiv - Neuroscience 2024Quote: Twenty µg of synaptosomal protein in a volume of 80 µl were added to each well of a Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies, 103792-100) coated with polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 20 µg of synaptosomal proteins were added to each well in a volume of 80 µl of a Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies, 103792-100) coated with polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated with primary antibodies for 48 h at 4°C (AXIN2 1:100, FZD1 1:100, LEF1 1:100, AQP1 1:100, OTX2 1:50, β-Catenin cell signaling 1:400, β-Catenin Dako 1:200), washed and subsequently incubated with secondary anti-mouse or anti-rabbit antibodies diluted in PBS (at 1:1000 ...
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bioRxiv - Bioengineering 2020Quote: Glucose and organic acids from cell cultures were measured by an 1100 Series HPLC system equipped with a 1200 Series refractive index detector (RID) (Agilent Technologies, Santa Clara, CA) and Aminex HPX-87H ion-exclusion column (300 mm length ...
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bioRxiv - Neuroscience 2021Quote: The effects of SCFA treatment on mitochondrial oxygen consumption rate (OCR) of differentiated SH-SY5Y cells were assessed using a Seahorse XFe96 analyzer (Agilent, Santa Clara, CA, U.S.A). In this assay ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were further incubated overnight in 1% NHS in TBSTx with rabbit anti-GFAP (diluted 1:500; Dako-Agilent, Santa Clara, CA, USA), rabbit anti-S100B (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were further incubated overnight in 1% NHS in TBSTx with rabbit anti-GFAP (diluted 1:500; Dako-Agilent, Santa Clara, CA, USA), rabbit anti-S100B (1:400 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 samples of myoepithelial cells and 5 samples of s-SHIP GFP+ cap cells were submitted and subjected to quality analysis using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). All Samples had an average RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and extracellular acidification rate (ECAR) were determined using a Seahorse XFe96 analyzer and the Seahorse XF Cell Mito Stress Test (Agilent Technologies, Santa Clara, CA). Cells were seeded into Seahorse XFe96 well plates at a density of 7500 cells/well/80 μl ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre-amplification-free single-cell whole genome sequencing libraries were prepared using a Bravo Automated Liquid Handling Platform (Agilent Technologies, Santa Clara, CA, USA), followed by size-selection and extraction from a 2% E-gel EX (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: Respiration and acidification rates were measured on monolayer culture of undifferentiated hPSCs and cells at S5 using a Seahorse XFe96 extracellular flux analyser (Agilent, Santa Clara, CA, US). Geltrex coated XFe96 assay plates were utilized for seeding of 3×103 undifferentiated hPSCs and 2×105 hPSC-derived cardiac cells in each well ...
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bioRxiv - Molecular Biology 2022Quote: ... Respirometry of isolated mitochondria from brain and SPC tissue and from cell cultures was performed using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience; Agilent, Santa Clara, CA). Mitochondrial oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2022Quote: ... and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Cell Energy Phenotype kit on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Oxygen consumption rate (OCR) of mitofusin mutants expressed using adenovirus in H9c2 cells was measured using a Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience, Billerica, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). 10-12 day old i3Neurons were treated with 5 nM vincristine ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture of early/late apoptosis and some necrotic death was induced by irradiating Jurkat cells and the isolated neutrophils under a UV lamp (254nm, Stratalinker UV 1800, Stratagene Corporation, La Jolla, CA) for 1 and 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... The sequence of bPAC-cMyc-IRES-mCherry was carried in pShuttle-CMV vector for generation of adenovirus (AdbPAC) in 293AD cells with the AdEasy system (Agilent Technologies, Santa Clara, CA) (34) ...
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bioRxiv - Immunology 2023Quote: ... The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured with an XF Cell Mito Stress Kit (Agilent, Santa Clara, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Primary cells isolated from parotid salivary glands were plated on an Agilent Seahorse XF96 Cell Culture Microplate (Part No. 101085-004, Agilent Technologies, Cedar Creek, TX) at a seeding density of 150,000 cells/well and cultured prior to irradiation at 24 hours or 48 hours before running the Seahorse ATP Rate Assay ...
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bioRxiv - Cancer Biology 2024Quote: Investigation of oxygen consumption rate/ extracellular acidification rate (OCR/ECAR) ratios and mitochondrial function were performed using XF96 analyzers with XF96 V3 cell culture microplates (Agilent, Santa Clara, CA, USA). FaDu and HaCaT cells were plated at 8000 cells per well and treated with bizine 24 hours after plating ...