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Citations for Agilent :
1701 - 1750 of 2011 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Endogenous OCR was measured in adherent SH-SY5Y cells using a Seahorse XF-24 Analyzer (Seahorse Bioscience, Billerica, MA, USA) following the manufacturer’s protocol with minor modifications (63) ...
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bioRxiv - Biochemistry 2023Quote: ... of C2C12 cells expressing UCP1 under the control of the doxycycline-inducible promoter (TRE3G) were measured with Seahorse XFe24 analyzer (Agilent). Cells were seeded at 4 x 104 cells per well in XFe24 cell culture microplates (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... medium was removed and cells were washed according to manufacturer’s protocol with Seahorse assay medium consisting of DMEM (103575-100, Agilent, USA) with 10 mM glucose ...
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bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
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bioRxiv - Bioengineering 2024Quote: The OCR and ECAR of hMSCs was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyzer (Agilent), in order to assess the mitochondrial respiration and glycolysis ...
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bioRxiv - Cell Biology 2024Quote: ... Concentration and insert size distribution for both the gene expression library and the cell multiplexing library were determined with a BioAnalyzer High Sensitivity DNA Assay (Agilent). Sequencing was performed on a NovaSeq 6000 on multiple flowcells with a paired-end 150 bp configuration ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Cancer Biology 2024Quote: Cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Cell Mito Stress Test in a XFe24 Bioanalyzer (Agilent). Cells were plated at a concentration of 30,000 (HF2303 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Pu.1 (1:200; 2258, Cell Signalling), hamster anti-CD3 (1:500; MCA2690, AbD Serotec) or rabbit anti-GFAP (1:2400, Z0334, Dako) diluted in the blocking solution overnight at 4°C plus one additional hour at room temperature (RT) ...
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bioRxiv - Molecular Biology 2024Quote: Data analysis for cell culture EVs was done using the Spectrum Mill MS Proteomics Workbench software package v 4.2 beta (Agilent Technologies). All extracted spectra were searched against a UniProt database containing human reference proteome sequences ...
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bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Primary mouse AT2s were isolated and plated as described above with measurement of mitochondrial respiration performed using the Seahorse XF Cell Mito Stress Test Kit (Agilent) or the Seahorse XF Palmitate Oxidation Stress Test Kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence and were used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2023Quote: ... Successful differentiation into cholangiocyte-like and hepatocyte-like cells was tested validated by immunostaining against albumin (Agilent, Cat. Nr. A0001). Primary human hepatocytes (PHHs ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed by NovoCyte 3000 flow cytometer equipped with three lasers and 13 fluorescence detectors (Agilent, Santa Clara, CA). GFP and Alexa Flour 647 fluorophores were excited by the 488 and 640 nm lasers ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed in 1X PBS and exposed to UV (10 J/m2) in a Stratalinker UV crosslinker oven (Stratagene).After UV exposure ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
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bioRxiv - Neuroscience 2023Quote: ... Primary fibroblasts plated in 96-well assay plate at a density of 40,000 cells/well were cultured overnight and then equilibrated with Seahorse XF base medium (Agilent Technologies) supplemented with glucose ...
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bioRxiv - Biochemistry 2022Quote: ... The sensor plate was calibrated in the machine prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 analyser (Agilent). Following assays ...
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bioRxiv - Neuroscience 2023Quote: Double immunofluorescence for activated pro- and anti-inflammatory microglial cells was performed as above with rat anti-CD86 (M1; 1:1600 dilution, Dako) and goat anti-CD206 (M2 ...
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bioRxiv - Immunology 2023Quote: ... and 1-2×105 T cells per well were attached in 5-8 replicates in Seahorse XF RPMI medium (Agilent) supplemented with 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: The oxygen consumption rate (OCR) of WT and TARBP1 KO cells was measured by using the XF96 extracellular flux analyzer (Agilent). 30000 cells were seeded onto one well of mini plates ...
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bioRxiv - Cell Biology 2023Quote: ... Vectors encoding the recombinant proteins were constructed as described in the previous section and introduced into the BL21-CondonPlus (DE3)-RIL competent cells (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... GEMs were used to generate barcoded cDNA libraries following the manufacturer’s protocol (Single Cell 3’ Reagent Kit v3.1, 10X Genomics) and quantified using the TapeStation High Sensitivity D5000 kit (Agilent, Germany). Subsequently ...
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Genomics 2023Quote: ... the size of the library of 2000 cells was measured using a High Sensitivity D1000 ScreenTape (Agilent Technologies, 5067-5584) on a TapeStation (Agilent Technologies ...
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bioRxiv - Pathology 2023Quote: ... and oxygen consumption rates (OCR, XF Cell Mito Stress Test) were performed with a Seahorse XF24 Extracellular Flux Analyzer and test kits (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The different instrumentation used corresponds to the two separate experimental batches (HySic Study I: A549 and NCI-H358 cell lines, H-FX-Agilent 1290 setup ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence in the plasmid used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: OCR and ECAR were measured in XF96 cell culture microplates using a Seahorse XFe96 Extracellular Flux Analyzer (Seahorse, Agilent Technologies). ISE6 cells were seeded at densities of 120 – 150,000 cells per well in complete L15C300 media41 and incubated at 34°C for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Agilent Seahorse XF Cell Mito Stress Test Kit was used to perform Mitostress test in the presence of 10mM glucose (Agilent) with addition of Oligomycin ...
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bioRxiv - Genomics 2023Quote: ... and mock-edited control cells (CTRL) were plated on poly-D-lysine coated 96-well Seahorse assay plates (XF96, Agilent) in DMEM (Gibco A144300 ...
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bioRxiv - Immunology 2023Quote: 3.5×105 HIF-1α-deficient or WT BMDCs were plated in Seahorse XF96 Cell Culture Microplates (Agilent Technologies, #101085-004). Cells were kept in 180 μL of assay medium (supplemented with 2 mM glutamine ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of live and dead cells in 30 µL was counted using a NovoCyte 2100YB Flow Cytometer (Agilent Technologies). Vincristine sulfate (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The sensor plate was calibrated prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 (Agilent). Following assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell Mito Stress Test was further combined with substrate pathway-specific inhibitors (etomoxir, UK5099, BPTES) (Agilent Technologies, Santa Clara, CA) to interrogate which of the three primary substrates (long chain fatty acids ...
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bioRxiv - Biochemistry 2023Quote: ... Successful mutation of isolated clones was verified by Sanger sequencing and clones were retransformed into BL21-CodonPlus (DE3)-RP competent cells (Agilent) for protein expression.
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bioRxiv - Biochemistry 2023Quote: His6-SUMO-tagged and His6-SUMO-SNAP-tagged Tm1 protein constructs and GST-Khc 1- 365 were expressed in BL21-CodonPlus(DE3)-RIL cells (Stratagene) by isopropyl β-D-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2023Quote: ... non-targeted BMDCs or HIF-1α-deficient BMDCs were plated in Seahorse XF96 Cell Culture Microplates (Agilent Technologies, #101085-004) and stimulated with 10 mM of L-LA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Measurements of real-time of oxygen consumption rates (OCR) were determined in whole cells using a Seahorse XF96 extracellular flux analyser (Agilent) as described previously (78) ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from HEK293T cells as described above and RIN determined to be 10 for all samples (Bioanalyzer 6000, Agilent). Libraries were constructed from 800 ng total RNA (NEBNext Multiplex Small RNA library prep set for Illumina ...
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bioRxiv - Plant Biology 2023Quote: PHDsuper-FN3VRN5 from Zm for XL-MS analysis was inserted into pEC-LIC-His-3C containing a hexa-histidine tag and 3C protease cleavage site at the N terminus and was expressed in BL21 CodonPlus (DE3)-RIL cells (Agilent) in LB medium ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell 3′ RNA-seq and single-cell ATAC-seq libraries were quantified on an Agilent Bioanalyzer with a high-sensitivity chip (Agilent), and Kapa DNA quantification kit for Illumina platforms (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... and 5.47*105 cells/cm2 were plated in each well of a poly-D-lysine-coated Seahorse XFe24 V7 PS Cell Culture Microplate (Agilent) or a 24-well tissue culture plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-incubated for 1 h and treated in Agilent Seahorse XF Base Medium (Agilent Technologies, cat#103334-100), which is optimized for extracellular flux assays and contains thiamine at 8.2 μM concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...