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Citations for Agilent :
1851 - 1900 of 2011 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and proton efflux rates (OCR/ECAR/PER) in media immediately surrounding adherent cells cultured in an XFe24-well microplate (Seahorse Bioscience). One day before analysis ...
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bioRxiv - Evolutionary Biology 2024Quote: The prepared carbon dots were studied using UV−vis (1 cm cell quartz cuvette) in a Cary 100 UV−vis spectrophotometer (Agilent Technologies) in DI water ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells under the indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia) and measured using an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
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bioRxiv - Neuroscience 2024Quote: RNA from sorted cells were analyzed on the Agilent 2100 Bioanalyser system using the Eukaryote Total RNA Pico kit (Agilent Technologies) and the mean RIN number was 9.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in primary antibody (anti-Alk1 Rabbit Abcam #ab68703, 1:100; anti-CD31 Mouse Dako #M0823, 1:500) over-night at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: OXPHOS and glycolysis of leukemia cells were assessed by extracellular flux using a Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, CA) with a Cell Mito Stress Test Kit (103015 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA from FACS-purified cells was quantified and assessed for integrity using a Bioanalyser 2100 and a RNA 6000 pico assay (Agilent Technologies). RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A ...
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bioRxiv - Immunology 2024Quote: ... were sequentially used to treat cells during real-time measurements of extracellular acidification rate (ECAR) using Seahorse XFe24 Analyzer (Seahorse Bioscience). For the mito stress test ...
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bioRxiv - Cell Biology 2024Quote: ... RPTECs were plated at 4 x 105 cells/well in a Seahorse 24-well V7 microplate (Seahorse Bioscience, Santa Clara, CA) and cultured in complete renal epithelial growth medium for 16-18 h in a 5% CO2 incubator at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates; Agilent Technologies) were then seeded with 80 µl of cell suspension (4.4×105 cells/ml ...
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bioRxiv - Microbiology 2024Quote: ... however with GFP Mouse mAb as a primary antibody diluted 1:1000 in PBST (Cell Signalling, 4B10) and Anti-mouse peroxidase (Dako, P0447) as a secondary antibody diluted 1:5000 ...
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bioRxiv - Immunology 2024Quote: ... or IL-4 and ABS-hi using Extracellular Flux Assay kits (XFE96 Flupax mini 102601-100 kit, Seahorse XF DMEM assay medium pack 103680-100 kit, XF cell mitostress test 103015-100 kit; all from Agilent Technologies) and Seahorse XF Pro Analyzer according to manufacturer’s recommendations and previously published protocol ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial respiration and glycolysis were determined by measuring OCRs and ECARs in the extracellular space with a Seahorse XF96 Extracellular Flux Analyzer with the XF Cell Mito Stress Test Kit and XF Glycolysis Stress Test Kit (Seahorse Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... and extracellular acidification rate (ECAR) were measured following the manufacturer’s instructions for the Seahorse XF Cell Mito Stress Test Kit (Agilent, Madison, WI). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... IHC antibodies: rabbit anti-EGFR (clone SP84, Cell Marque #14R-15) at 1:25 and mouse anti-Ki67 (clone MIB1, Dako #M7240) at 1:50 with ME Kit ...
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bioRxiv - Bioengineering 2024Quote: ... with 9 z-stack images were taken every 12 hours for 48 hours using a Cytation 5 cell imaging multimode reader (Agilent Technologies). The distance between the z-stack is described in each experiment ...
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bioRxiv - Bioengineering 2024Quote: ... two-channel 100 µm z-stack images were taken every 3 hours for 48 hours using a Cytation 5 cell imaging multimode reader (Agilent Technologies). Images were processed with NIH Fiji-ImageJ and cell coverage was measured for each cell type at every time point by calculating the area within the well covered by cells and dividing it by the total well area ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cell growth assays were performed via label free live cell imaging using the Cytation 5 Imaging Multi-Mode reader with attached BioSpa (Agilent BioTek). For 72 hr growth analysis cells were plated at ∼500 cells/well while for 144 hr growth analysis cells were plated at ∼100 cells/well ...
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bioRxiv - Immunology 2023Quote: Real-time analysis of oxygen consumption rates (OCR) were measured in THP-1 cells using XF24/96 Extracellular Flux Analyzer (Seahorse Bioscience). Cells were seeded in Seahorse XF96 cell culture microplate (#101085-004 ...
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bioRxiv - Neuroscience 2023Quote: ... of VM cells cultured with the PIEZO1 antagonist GsMTx4 and the PIEZO1 agonist Yoda1 was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyser (Agilent®), in order to assess the mitochondrial respiration and glycolysis ...
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bioRxiv - Systems Biology 2023Quote: The kinetic data in this study are published and were acquired from xCELLigence assays using the xCELLigence Real Time Cell Analysis (RTCA) Single Plate device (Agilent Technologies) (Dankwa et al ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was stored at −80°C and RNA quality was assessed by Bioanalyzer (Agilent 2100 Bioanalyzer, RNA 6000 Nano Kit for cells, RNA 6000 Pico Kit for EVs, Agilent Technologies).
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bioRxiv - Immunology 2023Quote: ... 1.5 µM FCCP and 1 µM Rotenone/1 µM Antimycin A from Seahorse XF Cell Mito Stress Test (103015-100, Agilent Technologies), and read using Seahorse XFe96 Analyzer (Agilent Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... Hela CD95KO and Hela WT stable CD95-mEGFP was assessed using the QIFIKIT® for quantification of cell surface antigens by flow cytometry (K007811-8, Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The real-time measurements of OCR of H460-FASN-WT/-KO cells were detected on a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies). 1×10^4 cells/well were incubated with XF assay medium (pH 7.4 ...
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bioRxiv - Cancer Biology 2023Quote: Murine glioma cells (similar low passage N1IC-1/2 and p53-1/2) were seeded in PLL coated XFe24 cell culture microplates (Agilent TEchnologies) at 1.8ᵡ104 cells per well in 250μL BFP (n=10 technical replicates for the baseline experiment and n=5 technical replicates for the cysteine/methionine deprivation experiment ...
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bioRxiv - Genomics 2023Quote: Cells were allowed to grow to confluency and UV crosslinked using 254nm UV light in Stratalinker 2400 on ice (Stratagene, USA). UV treated cells were lysed to get different cellular fractions (Cytoplasmic ...
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bioRxiv - Cancer Biology 2023Quote: ... HMLER CD24low were seeded at a density of 104 cells per well in a specialized 96-well Seahorse XF96 V3 PS microplate (101085-004, Agilent Technologies). 48h after ...
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bioRxiv - Biochemistry 2023Quote: The concentration of iron in the cell cultures was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Agilent, 5110). Freeze-dried cell samples were digested in concentrated nitric acid at 80 °C overnight.
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bioRxiv - Biochemistry 2023Quote: ... was used as a template to generate deletion variants for expression in insect cells using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). For bacterial expression of the ΔN790 variant ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Molecular Biology 2023Quote: Oxygen consumption and Extracellular acidification rates (OCR and ECAR respectively) were measured in MDA-MB-231 cells using the XF96 Extracellular Flux analyzer (Seahorse Bioscience). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated with primary antibodies (chicken anti-Map2 [Thermo Scientific, PA1-16751, 1:1000] and rabbit anti-Gfap [Dako, Z0334 ...
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bioRxiv - Immunology 2023Quote: The oxygen consumption rate (OCR) of treated cells was measured using an Xfe96 Extracellular Flux analyzer (Agilent, Santa Clara, CA, USA). One day before the experiment ...
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bioRxiv - Microbiology 2023Quote: ... all cultures were diluted in EMM(561 mM NH4Cl)+Leu+Ade+ura to seed an OD600nm at 0.01 into a poly-lysine coated Seahorse XFp cell culture miniplate in 50 μL of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Cancer Biology 2023Quote: ... The aqueous samples were resuspended in 35x the packed cell volume using 80:20 methanol:water and transferred to polypropylene mass spectrometry vials (Agilent, 5188-2788).
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 100,000 cells were counted at each experimental point using a NovoCyte Flow cytometer (Agilent, Santa Clara, CA, USA). DHR-stained cells were detected using 488 nm for excitation and 530/30 nm for emission.
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bioRxiv - Cell Biology 2023Quote: Mitochondrial respiration (JO2) of intact cells were measured using an XF HS mini Analyser Extracellular Flux Analyzer (Seahorse Bioscience, Copenhagen, Denmark). L6 myoblasts were seeded and differentiated in Seahorse XFp culture plates coated with matrigel and assayed after incubation at 37°C without CO2 for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Physiology 2024Quote: ... the cell pellet was resuspended in 500ul of warm MAS buffer solution [58] + 1nM Plasma Membrane Permeabilizer (Agilent Seahorse XF PMP) to permeabilize the cells ...
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bioRxiv - Biochemistry 2024Quote: ... pT7-7 α-syn FL plasmid (a gift from Hilal Lashuel, Addgene66) was transformed into BL21-Gold (DE3) competent Escherichia coli (E. coli) cells (Agilent Technologies) according to the manufacturer’s instructions and previous methodology44 ...
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bioRxiv - Neuroscience 2024Quote: Ten µg of mitochondrial protein in a volume of 30 µl were added to each well of a Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies) coated with polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe96 Extracellular Flux Analyzer (Agilent Technologies, California, USA) via changes in the Oxygen Consumption Rate (OCR ...
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bioRxiv - Biophysics 2024Quote: OCR measurements were performed with the Agilent Seahorse XF Cell Mito Stress Test kit with an XF24 extracellular flux analyser (Seahorse Bioscience). XF24 plates were coated with either 23 µl of 100% Matrigel (soft ...
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bioRxiv - Neuroscience 2024Quote: ... except that the dissociated spinal cord cells were plated in 80 µL serum-containing plating medium (50% DMEM, 25 % HBSS and 25 % horse serum) at 80.0000 cells per well in Seahorse XFp Miniplates (Agilent, 103025-100). After two hours ...
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bioRxiv - Microbiology 2024Quote: ... Cell densities were determined in two technical replicates with a NovoCyte Flow Cytometer equipped with a NovoSampler Pro (Agilent, Waldbronn, Germany). Two times 200 µL of each sample were pipetted into a 96-well microtiter plate (Sarstedt ...