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Citations for Agilent :
1501 - 1550 of 2011 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cell maintenance medium was exchanged with XF DMEM Base Medium (pH 7.4) with no phenol red (Agilent) supplemented with 1mM sodium pyruvate (Corning Cellgro) ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary VSMCs (2x104-3x104) were seeded onto to an Xfe96 poly-D-Lysine (PDL) Cell Culture Plate (Agilent) in 80uL of 5% FBS ...
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bioRxiv - Cell Biology 2023Quote: The oxygen consumption rate in cybrid cell lines was detected using an XFe24 extracellular flux analyzer (Seahorse Bioscience) as described59 with some modifications ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS and medium exchanged for Seahorse XF Base Medium Minimal DMEM (Agilent Technologies, #102353) supplied with 10 mM glucose ...
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bioRxiv - Immunology 2024Quote: ... 4×105 treated NK cells were resuspended in Seahorse XF DMEM Medium containing glutamine (1 mM; Agilent, USA) and seeded into an 8-well XF cell culture microplate (Agilent ...
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bioRxiv - Immunology 2024Quote: ... Dual luciferase activity was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent, Santa Clara, CA). Normalized luciferase activity was determined by calculating Firefly to Renilla signal ratio.
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bioRxiv - Physiology 2024Quote: ... we performed the bioenergetic analysis using the Seahorse T-cell Mitochondrial profiling kit (part. no.: 103771-100, Agilent) with a final concentration of 4mM Oligomycin ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated for 2h with primary antibody diluted in Blocking Buffer with 5% Goat Serum (Dako #X090710) (antibodies are dilutions used are listed in Table 2) ...
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bioRxiv - Biophysics 2021Quote: Room-temperature (RT) absorption spectra of cells were acquired on a Varian Cary 4000 UV–Vis-spectrophotometer (Agilent technologies) equipped with an integrating diffuse reflectance sphere (DRA-CA-50 ...
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bioRxiv - Biophysics 2021Quote: ... Mutations were introduced through site-directed mutagenesis (QuikChange II XL, with 10 XL Gold cells; Agilent Technologies, Kista, Sweden) and confirmed by sequencing at the Linköping University Core Facility.
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bioRxiv - Cell Biology 2020Quote: ... 2 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 0.5 μM rotenone + 0.5 μM antimycin from the Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies). Data was analyzed by the Seahorse XF Cell Mito Stress Test Report Generator ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR products of positive clones were then cloned into competent cells using the StrataClone PCR Cloning Kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... A specialised flow cell was employed in conjunction with an inline Agilent 1200 HPLC system (Agilent Technologies, Stockport, UK); sense and antisense AluSx1 RNA samples were injected onto a Shodex KW403-4F (Showa Denko America Inc. ...
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bioRxiv - Genomics 2020Quote: ... The ratio between cell-free DNA and high-molecular weight DNA (HMW) was calculated with the Prosize 3.0 software (Agilent). To this end ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial function was assessed in live cells using an XFp Extracellular Flux Analyzer (Agilent Technologies, Santa Clara, CA, USA). After 24 hours silencing (siNeg vs siCFH ...
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bioRxiv - Biochemistry 2019Quote: ... encoding N-terminally 6xHis tagged MBP-MS2 (Gift from Nadège Liaud, UC-Berkeley) was transformed into BL21 Codon+ RIL chemically competent cells (Agilent). An overnight culture was used to inoculate 3L of ZYM-5052 auto-inducing media (Studier ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... FACS sorted MPs were plated on XF96 cell culture microplates coated with 10% Matrigel in warm assay medium (Agilent), the cell culture plate was centrifuged with 200 g for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Seahorse records were corrected for well-to-well differences in the cell numbers and analyzed using Wave software (Agilent). Leukemia cells (40,000 cells/well for cell lines ...
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bioRxiv - Cancer Biology 2020Quote: ... A549N cells were seeded in normal media in Seahorse xFP plates (Cat# 103022-100, Agilent Technologies, Santa Clara, CA) 24 h prior to conducting the assay ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2×107 HeLa cells were crosslinked on ice with 0.8 J/cm2 UV 254 nm in a UV Stratalinker 2400 (Stratagene), washed in PBS with 2 mM EDTA and 0.2 mM PMSF ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Cancer Biology 2020Quote: Untargeted metabolomics profiling of PCK1-overexpressing cells was performed using ultra-high-performance liquid chromatography (Agilent 1290 Infinity LC) coupled to with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... Subsequently we strictly adhered to the Seahorse XF Cell Mito Stress Test Kit protocol (Agilent Technologies Cat# 103015-100). Cells were counted before and after the assay using a Celigo S Image cytometer (Nexcelom Biosciences ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... at 22.4µg/ml concentration per well to prepare Cell-Tak coated XF96 microplates according to the manufacturers guidelines (Agilent Technologies). Cells were seeded into the coated microplates immediately before the assay by centrifugation with 200g for 1min without brake ...
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bioRxiv - Cell Biology 2021Quote: ... Single cells were separated using a 100 μm strainer and labelled with CD31 antibody (1:200, M082329-2, DAKO) at 4°C for 45 min followed by 30 min staining with a goat anti-mouse secondary antibody conjugated to AlexaFluor 488 or 555 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 104 cells were seeded into each well of a V3 96-well plate (Agilent, Cat. # 101085-004) and cultured 24 h before measuring OCR ...
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bioRxiv - Cell Biology 2020Quote: ... The number of cells per well was directly incorporated into the Wave software (Seahorse Wave Controller 2.4 software, Agilent) to normalize OCR and ECAR values ...
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bioRxiv - Bioengineering 2020Quote: ... VO2max = 1.04×10−16 mol/s/cell was measured for hiPSC-Heps using the Seahorse XF24 cellular respirometer (Agilent) (see ...
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bioRxiv - Cell Biology 2021Quote: Oxygen consumption rates (OCR) from differentiated SVF cells were analyzed by the Seahorse XF24 Extracellular Flux Analyzer - (Agilent Technologies). Cells were seeded ...
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bioRxiv - Cancer Biology 2022Quote: ... and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies) every six hours for a total of six days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Isolated mouse cardiomyocytes were seeded at 2K per well in the Seahorse XF96 cell culture microplates (Agilent Technologies, 102416100) pre-coated with laminin mouse protein at 2μg/cm2 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were overlaid with 175 μL of mitochondrial assay solution [33] supplemented with the Seahorse Plasma Membrane Permeabilizer (Agilent), 4 mM ADP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: iNeurons were replated on DIV 4 onto polyornithine/laminin coated in the Seahorse XF96 Cell Culture Microplate (Agilent Technologies) at a density of 50 000/well ...
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bioRxiv - Neuroscience 2019Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience) via changes in the oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2019Quote: ... harvested after 72 hours of culture and plated on 96-well XF cell culture microplates in XF assay medium (pH 7.4, both from Agilent) supplemented with D-glucose (20mM ...
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bioRxiv - Biochemistry 2021Quote: ... These data were then normalized to the corresponding DNA/protein/cell number in the Wave data analysis software (Agilent) and exported to Microsoft Excel.
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cryopreserved tumour samples or cultured cells using the Total RNA Isolation Micro kit (Agilent) and cDNA then synthesized using SuperScript VILO cDNA synthesis kit (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... and centrifuged again to remove the supernatant.RNA was then extracted from the isolated disc cells using Absolutely RNA Nanoprep Kit (Stratagene), following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... the fragments were joined by Gibson assembly and used to transform chemically competent BL21-Gold(DE3) cells (Agilent Technologies). Individual clones were sequence-verified by Sanger sequencing (Microsynth AG) ...
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bioRxiv - Molecular Biology 2019Quote: ... N≥50 worms (10 worms/ well) were transferred to the Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) in a total volume of 180 uL ...
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bioRxiv - Cell Biology 2020Quote: Oxygen consumption rates (OCR) were measured in iBAs cells using a Seahorse XFe96 (Agilent Technologies, Santa Clara, CA, USA). Briefly ...
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bioRxiv - Biochemistry 2021Quote: HMECs were cultured at a density of 3 x 104 cells/well in 24-well Seahorse XFe24 plates (Agilent) and incubated at 37 °C under a humidified 5% CO2 atmosphere overnight in the presence or absence of DMF ...
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bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked in 0.2% triton X-100 solution containing 1% BSA and 5% normal goat serum (DAKO, X0907) for 1 hour.
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bioRxiv - Developmental Biology 2021Quote: ... at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat. no. 103575-100) supplemented with 10mM glucose (Agilent cat ...
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bioRxiv - Cell Biology 2020Quote: Forty-thousand HUVECs representing each genotype (0RV, 1RV, and 2RV) were seeded on V7 cell culture plates (Seahorse Bioscience). Cells were either left untreated or treated with IFNɣ (50pg/mL ...