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Citations for Agilent :
1701 - 1750 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and rotenone/antimycin A provided with the XF Cell Mito Stress Test Kit (#103015-100, Seahorse Bioscience) were prepared in XF assay medium (100840-000 ...
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bioRxiv - Cancer Biology 2020Quote: ... baseline GFP intensity was collected using the BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies), and subsequently 48-hour conditioned media from HEK293 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were stained with DAPI and slides were mounted with DAKO mounting medium (Agilent S302380-2). Images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems) ...
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bioRxiv - Developmental Biology 2019Quote: The metabolic function of cells was assessed by extracellular flux analysis using Seahorse XF24 (Agilent Technologies, UK). On the day prior to the assay ...
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bioRxiv - Cell Biology 2021Quote: ... A53T and E46K α-synuclein proteins were expressed in BL2-Gold(DE3) competent cells (Agilent, Cat #230132). Protein expression was induced at log phase with 200 μM IPTG for 16 hours at room temperature ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... mouse (Fig. S8b) and naked mole-rat (Fig. 2a-c) marrowSeahorse XF96 Cell Culture Microplates (Agilent Technologies) between 50-250*103 cells per 200µl of the following culture media ...
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bioRxiv - Molecular Biology 2021Quote: OCR of HepG2 cells were analyzed by Seahorse XF24 extracellular flux analyzer (Seahorse Bioscience, North Billerica, MA) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Cellular respiratory capacity was assayed using the XF Cell Mito Stress Test Kit (Agilent Technologies, Inc., USA). The assay consists of repeated cycles of 3-min mix ...
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bioRxiv - Immunology 2020Quote: Seahorse XF Cell mito stress test assays were done using a Seahorse XFe96 Extracellular Flux Analyzer (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was conducted in E.coli BL21 (DE3) Codon Plus cells (Agilent Technologies, San Diego, CA, USA). Induced cultures were grown at 18° C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... and transformed into Agilent XL1-Blue Electroporation-Competent Cells (Agilent, Santa Clare, CA, cat # 50-125-045) by electroporation using Gene Pulser Xcell electroporation system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... and their respective point mutants were overexpressed in Escherichia coli BL-21 or TKB1 competent cells (Stratagene) (100 mL Luria Bertani (LB ...
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bioRxiv - Neuroscience 2022Quote: ... pFBAAV2-CMVP.NpA (obtained from University of Iowa Gene Transfer Vector Core) and grown in Sure2 cells (Stratagene). The rAAV was produced by the University of Iowa Viral Vector Core Facility.
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bioRxiv - Cancer Biology 2022Quote: ... Mitochondrial respiration was studied by monitoring OCR using Seahorse Cell Mito Stress Test kit from Seahorse Bioscience and glycolytic activity was studied by monitoring ECAR using Seahorse Glycolysis Stress Test Kit from Seahorse Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... according to the manual of Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, cat#103015-100). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed once and incubated in Seahorse XF base medium (pH7.4, Agilent Technologies, cat#103334-100) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Microbiology 2022Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 μg of each plasmid was used to transfect ring stage parasites ...
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bioRxiv - Molecular Biology 2023Quote: ... Before sorting islet cells were filtered through a 35 µm filter and sorted using MoFlo Cytometer (Dako), where cells were gated according to forward scatter and then sorted based on endogenous fluorescence (Smelt et al. ...
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bioRxiv - Physiology 2023Quote: ... BMSC purified with MACS were seeded at 2 × 105 cells/cm2 into XF96 tissue culture microplates (Agilent) at 24 hours prior to experiments ...
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bioRxiv - Microbiology 2022Quote: ... Optical density was read at 405 nm on a Cytation 3 Cell Imaging Multi-Mode Reader (Agilent) and analysed in Bioek Gen5 (Agilent).
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bioRxiv - Cell Biology 2022Quote: ... while oxygen consumption rate (OCR) assessed using a Cell Mito Stress Test kit (Agilent Technologies, 103015-100). ECAR and OCR were determined with an XF24 Extracellular Flux Analyzer (Seahorse Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested by trypsinization and lysed in RNA lysis buffer following the RNA Miniprep kit (Agilent). Genomic DNA contamination was reduced by on-column DNase digestion as described in the manual ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... AAV9-CAG-tdTomato and AAV-KP1-CAG-tdTomato were produced in HEK293 cells (RRID: CVCL-6871, Agilent) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride density-gradient ultracentrifugation followed by dialysis as described previously.53 AAV9 and AAV-KP1 helper plasmids were provided by J ...
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bioRxiv - Immunology 2023Quote: Mitochondrial respiration was assessed using the Seahorse Cell Mito Stress Test in a Seahorse Analyzer XFe24 (Agilent) with some adjustments to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: The Seahorse XF Cell Mito Stress Test was performed in a XFe96 Seahorse extracellular flux analyzer (Agilent). ATP production rates derived from glycolysis (JATP-Glyc ...
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bioRxiv - Neuroscience 2023Quote: ... were measured from live cells in real time using Seahorse Extracellular Flux (XF) 24 Analyzer (Agilent Technology). OCR and ECAR were measured simultaneously ...
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bioRxiv - Cell Biology 2023Quote: Measurement of mitochondrial respiration was done using a Seahorse XF Cell Mito Stress Test kit (Seahorse, Agilent) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice in PBS before changing to Seahorse XF base media (Agilent, 102353-100). Mitochondrial stress tests were run with the following concentrations of media ...
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bioRxiv - Neuroscience 2023Quote: ... was used to stain the nucleus before the cells were mounted using DAKO fluorescent mounting medium (DAKO). For the surface GluA2 staining and axon initial segment staining ...
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bioRxiv - Cell Biology 2023Quote: ... Seahorse XF Cell Mitostress test and Real-Time ATP Rate Assays (Agilent Technologies, Santa Clara, CA, USA) were used to evaluate mitochondrial function ...
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bioRxiv - Microbiology 2023Quote: ... Cellular fatty acids were extracted and determined from dried cells by using GC (model 7890A, Agilent, USA) according to the protocol of the Sherlock Microbial Identification System (61) ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... Cells were washed 3x with 0.5% BSA and mounted onto glass slides with fluorescent mounting media (DAKO) for imaging.
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bioRxiv - Neuroscience 2023Quote: ... rAAVs were produced by co-transfection of human embryonic kidney (HEK) cell line 293 (Stratagene, California, USA) with the target AAV plasmid and helper plasmids (pFΔ6 ...
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bioRxiv - Microbiology 2023Quote: Cellular respiration was measured using the Seahorse XF Cell Mito Stress Test Kit (Agilent, Santa Clara, CA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: 1 x 105 BMDMs were seeded in Agilent Seahorse XF24 Cell Culture Microplate (Agilent Technologies, 100777-004) and treated as required by experiments ...
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bioRxiv - Immunology 2023Quote: ... Images were acquired (1-5 images per subject) using the Cytation 5 Cell Imaging MultiMode Reader (Agilent). Images were processed to quantify fluorescence intensity using Gen5 software package 3.08.
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bioRxiv - Microbiology 2023Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 µg of pDC2-MORC-HA or linearized pKD-MORC-HA-TetR-DOZI ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in ice-cold 400 µl FACS buffer and measured using NovoCyte Quanteon system (Agilent). Data analysis was performed using FlowJo v10.6.2 software ...
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bioRxiv - Cell Biology 2024Quote: The Nir1-LNS2 plasmid was transformed into competent BL21 (DE3) RIPL cells (Agilent Technologies, Cat. No. 230280) for protein overexpression ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with Seahorse XF DMEM assay medium (#103680-100, Seahorse Bioscience, Agilent Technologies, US) for 1 h at 37°C in absence of CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with Seahorse XF DMEM assay medium (#103680-100, Seahorse Bioscience, Agilent Technologies, US) for 1 h at 37°C in absence of CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were analyzed on a fluorescent plate reader (Biotek Synergy HTX, Agilent Technologies, Inc., Santa Clara, CA) at excitation of 485/20 nm and emission 528/20 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x106 cells were divided in two fractions- for FITC labelled Telomeric PNA probe (Agilent DAKO kit) and unlabelled control cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x106 cells were divided in two fractions- for FITC labelled Telomeric PNA probe (Agilent DAKO kit) and unlabelled control cells ...
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bioRxiv - Biochemistry 2024Quote: ... cells were exposed to UV light (365 nm) in a UV cross-inker (Stratagene, UV Stratalinker 1800) placed in cold room for 10 min ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... NPCs were plated directly in 96-well Agilent Seahorse Cell Culture plates (Agilent Technologies, Cat#: 101085-004), coated with Matrigel ...
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bioRxiv - Cell Biology 2024Quote: ... 20.000 CD34+ were plated for each precoated well of the Seahorse XFp mini Cell Culture Plates (Agilent) precoated with rhLaminin-521 (Thermo- Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Quality control of cDNA and single-cell libraries were analyzed using Agilent 4150 tape station (Agilent Technologies). Dual indexed single-cell libraries were pooled ...
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bioRxiv - Cell Biology 2024Quote: ... the Seahorse Cell Mito Stress Test profile was performed using the Seahorse XFe96 Extracellular Flux Analyzer (Agilent Scientific Instruments ...
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bioRxiv - Biochemistry 2024Quote: ... cells were exposed to UV light (365 nm) in a UV cross-inker (Stratagene, UV Stratalinker 1800) placed in a cold room for 10 min ...