Labshake search
Citations for Agilent :
1501 - 1550 of 2290 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... the pET21 plasmid containing PL1 was transformed into BL21 DE3 pLyS cells (Agilent, Edinburgh, UK). PL1 expression was induced at mid-log phase by supplementing the media with 0.3 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) ...
-
bioRxiv - Cell Biology 2019Quote: RNA was extracted from mouse embryonic stem cells using the Absolutely RNA microprep kit (Agilent). 1 µg of RNA was pre-incubated with 4.5 µg of (PR)20 or water for 10 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: RNA extraction from FACS-isolated cells was performed using the Absolutely RNA nanoprep kit (Stratagene) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2021Quote: Assays were performed using a Seahorse XF96 Cell Mito Stress Test Kit (Agilent Biosciences, USA). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were resuspended in 200 μL PBS containing 1% BSA for detection by NovoCyte (Agilent) utilizing laser excitation and emission wavelengths of 488 nm and 530 nm ...
-
Airway Gene-Expression Classifiers for Respiratory Syncytial Virus (RSV) Disease Severity in InfantsbioRxiv - Immunology 2020Quote: ... Recovered cells were lysed and homogenized using the AbsolutelyRNA Miniprep kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
-
bioRxiv - Immunology 2020Quote: ... cells were barcoded using automated liquid handling robotic system (Agilent Technologies, Santa Clara, CA, USA)(38 ...
-
bioRxiv - Immunology 2021Quote: ... 1.5 x 105 cells per well were seeded into seahorse culture plate (Agilent, 102416-100) precoated with Cell-Tak (Corning ...
-
bioRxiv - Genetics 2021Quote: ... All compounds were from a Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100). Four OCR measurements were recorded for baseline and following each injection ...
-
bioRxiv - Biochemistry 2020Quote: ... The hSirt3,102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE) as per manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2021Quote: ... cell culture media was washed and replaced with fresh assay media (XF basic media; Agilent), supplemented with 10 mM Glucose ...
-
bioRxiv - Genetics 2019Quote: ... The PCR product was ligated with T4 ligase prior to transformation into competent cells (Stratagene). The plasmid was sequenced to validate the removal of the putative EGR1 binding site ...
-
bioRxiv - Neuroscience 2021Quote: ... AAV8- GFAP-OptoA1-eYFP was produced by standard triple transfection of AAV 293 cells (Agilent). At 72 h post-transfection ...
-
bioRxiv - Physiology 2021Quote: ... The cells were washed twice with 0.5 ml Seahorse XF DMEM medium pH 7.4 (Agilent) and then incubated in a CO2-free incubator at 37 °C (or 24°C for S2 cells ...
-
bioRxiv - Immunology 2020Quote: ... BMDCs (80,000 cells/well) were analyzed with an XF-96 Extracellular Flux Analyzer (Seahorse Bioscience). 1-2 x 105 cells/well were used in 96-well tissue-culture treated flat bottom plates for the metabolic measurements ...
-
bioRxiv - Immunology 2021Quote: ... monocytes were seeded as a monolayer onto XFe24 cell culture plates (Seahorse Bioscience, MA, USA). The culture media was replaced with XF assay media supplemented with l-glutamine (250 μg/ml ...
-
bioRxiv - Immunology 2021Quote: ... the cell culture medium was replaced with XF RPMI medium pH 7.4 (Agilent, 103576-100) supplemented with 2 mM glutamine prior to analysis ...
-
bioRxiv - Cancer Biology 2021Quote: PyMT cells were seeded into Seahorse XFe-96 sensor plates (Agilent Technologies, Santa Clara, CA) to produce near confluence within 18h ...
-
bioRxiv - Cancer Biology 2020Quote: ... Adenoviral plasmid was linearized by PacI digest and transfected into AD-293 cells (Agilent Technologies) to produce recombinant viral particles ...
-
bioRxiv - Cancer Biology 2022Quote: Oxidative respiration was measured using XF Cell Mito Stress Test Kit (Agilent Technologies, 103015-100). 1 x 104 cells per well were seeded on an XF96 Cell Culture Microplate ...
-
bioRxiv - Molecular Biology 2022Quote: ... Conventional sequencing transformations into XL1-Blue Super competent cells (Agilent Technologies, Santa Clara, CA, USA) were used for all ligations ...
-
bioRxiv - Cancer Biology 2022Quote: Proliferation of cells in real time was monitored using the xCELLigence RTCA SP instrument (Agilent). 2500-10,000 cells per well were seeded at the start of the assay ...
-
bioRxiv - Cancer Biology 2022Quote: Oxidative respiration was measured using XF Cell Mito Stress Test Kit (Agilent Technologies, 103015-100). 1 × 104 cells per well were seeded on an XF96 Cell Culture Microplate ...
-
bioRxiv - Cancer Biology 2022Quote: ... Hydrophobic barriers were drawn around dried cell zones using a DAKO pen (DAKO, Cat. # S2002). Cells were fixed in 4% paraformaldehyde (Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Melanoma cells were seeded in quintuplicates on a Seahorse XF Microplate (Agilent, Cat# 100777-004) with melanoma culture medium supplemented with the corresponding drugs ...
-
bioRxiv - Cell Biology 2022Quote: ... The E-plate was engaged into the xCELLigence Real Time Cell Analysis (RTCA) Instrument (Agilent) and background measurement of the wells plus growth media was recorded before adding cells in 150 μL of media for each SLC condition (5 x 103 cells/well) ...
-
bioRxiv - Immunology 2022Quote: The Seahorse XF Cell Mito Stress Test Kit (Agilent, Santa Clara, CA; Catalog #103015-100) was run on a Seahorse XFe96 Bioanalyzer (Agilent ...
-
bioRxiv - Immunology 2022Quote: ... glycolytic agents (Seahorse Bioscience Cell Glycolysis Stress Test, Agilent #103017-100, Santa Clara, CA, USA) were utilized at 10 mM D-glucose ...
-
bioRxiv - Microbiology 2022Quote: ... Barcoded single-cell capture plates were prepared with a Bravo automated liquid handling platform (Agilent). For generation of the RNA library ...
-
bioRxiv - Physiology 2023Quote: ... Hepatocytes were then cultured in collagen type I coated XF24 cell culture microplates (Seahorse Bioscience) at 1.5 ×104 cells per well and incubated 4-6 h at 37 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were UV-crosslinked with 150 mJ/cm2 at 254 nm (stratalinker UV crosslinker, Stratagene) on ice ...
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... Punches were ejected directly into an XFe96 Cell Culture Microplate (Agilent Seahorse XF, XF96 FluxPack) based on a pre-determined plate layout ...
-
bioRxiv - Molecular Biology 2023Quote: ... and cell numbers were determined with the Cytation 5 (Biotek Instruments, Agilent, Winooski, VT USA). OCR and ECAR data were expressed as pmol/minute/103 cells and mpH/minute/103 cells ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were collected by centrifugation and resuspended in supplemented Seahorse XF RPMI (Agilent 103576-100) at ∼8 x 105/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 to 15 adult worms were transferred to a Seahorse XF96 Cell Culture Microplate (Agilent) with 200 μL of M9 buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed in PBS tween and mounted using fluorescent mounting medium (Agilent Dako, #S3023) for imaging ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed in PBS tween and mounted using fluorescent mounting medium (Agilent Dako, #S3023) for imaging ...
-
bioRxiv - Biophysics 2023Quote: Halo-tagged dCas9 protein expression was induced in BL21-CodonPlus(DE3)-RIL cells (Agilent #230245) transformed with pET302-6His-dCas9-halo (Addgene #72269 ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... transfection mixtures for split cell culture samples contained either pBlueScriptII KS (pBS, Stratagene, Heidelberg, Germany) or wild-type EGFP expression plasmid ...
-
bioRxiv - Cell Biology 2022Quote: Primary subcutaneous adipocytes and brown adipocytes were plated into 96 well cell culture microplates (Agilent) and cells were plated in seahorse XF base medium (Agilent ...
-
bioRxiv - Microbiology 2023Quote: ... expression plasmids described above were transformed into BL21(DE3) or BL21(DE3)-RIL cells (Agilent), plated on MDG media plates (1.5% Bacto agar ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with the appropriate primary antibody detailed above diluted in Dako (Agilent) antibody diluent to 1:500 ...
-
bioRxiv - Immunology 2023Quote: The Seahorse XF Cell Mito Stress Test Kit (Agilent, Santa Clara, CA; Catalog #103015-100) was run on a Seahorse XFe96 Bioanalyzer (Agilent ...
-
bioRxiv - Immunology 2023Quote: ... Antibody-stained cells were analyzed immediately by flow cytometry using the Novocyte Advanteon instrument (Agilent), and the data were analyzed using FlowJo software ...
-
bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
-
Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... seeded (25,000-50,000 cells/well) on Matrigel coated test plates (Extracellular Flux Assay Kit, Agilent) and cultured 4 days in maturation medium prior to measurement ...
-
bioRxiv - Neuroscience 2024Quote: ... Schwann cells were labeled with rabbit anti-cow S-100 polyclonal antibody (Dako, Carpentaria, CA). After incubation with the primary antibodies ...
-
bioRxiv - Physiology 2024Quote: ... cells were bathed in glucose-free HBSS containing a Rotenone/Antimycin cocktail (1μM, Agilent Technologies) and 10 mM 2-deoxy-D-glucose for 60 mins at room temperature ...