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Citations for Agilent :
1301 - 1350 of 1386 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and ORAI1 (c.319G>A; p.V107M) point mutations were introduced by site-directed mutagenesis using the Pfu DNA polymerase (Stratagene, La Jolla, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... column maintained at ambient temperature (30ΰC) coupled to a quadrupole time-of-flight (Q-TOF) mass spectrometer (Q-TOF-LC/MS 6510 classic G6150A, Agilent technologies, USA) with a dual ESI as ion source ...
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bioRxiv - Microbiology 2021Quote: ... for 1 hour at room temperature or overnight at 4°C using the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) technique according to the manufacturer’s protocol (Dako, Carpinteria, CA, USA). All antibodies were diluted in TBS with 0.1% BSA ...
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bioRxiv - Synthetic Biology 2019Quote: ... Injections of 5 µL of each sample were separated using a Zorbax Eclipse Plus C-18 column (2.1 × 100 mm, 1.8 µm) (Agilent, Santa Clara, CA, USA) at a flow rate of 0.35 mL/min ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue sections were then incubated overnight at 4°C with primary antibodies (Extended Table 2) in Antibody Diluent (Agilent Dako, S080983-2). Following a wash step ...
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bioRxiv - Developmental Biology 2022Quote: ... The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000, rabbit anti-fibronectin, DAKO, Hamburg, Germany). After washing with TBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... was carried out by drying silica NPs at 37°C for 48 hours and finally placing the dry sample onto a Cary 630 FTIR spectrometer (Agilent Technologies Ltd) for the measurement of transmittance spectra ...
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bioRxiv - Biophysics 2022Quote: Fluorescence titrations were carried out at 25 °C using 1 × 0.3 cm path-length cuvettes in a Cary Eclipse spectrofluorimeter (Agilent Technologies, Waldbronn, Germany). Experiments were carried out in 20 mM K-phosphate ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence was measured at 50°C over 1 h at excitation 360 nm emission 460 nm on a BioTek Synergy HTX Multimode Reader (BioTek, Agilent, Tx, USA). The reaction rate was calculated as the maximum velocity over 12 sequential readings ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... seedlings were exposed on the plates to 8 kJ/m2 UV-C light in a Stratalinker 2400 (Stratagene, La Jolla, California, US) and transferred back into growth chamber for 5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Physiology 2023Quote: ... slides (4 μm thickness) were baked at 60° C for 45 min and treated with a low pH target retrieval solution (#DM829; Dako, Glostrup, Denmark) in a DAKO PT Link HIER machine (PTlink 200 ...
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bioRxiv - Genomics 2023Quote: ... and the size and concentration of the finished PCHi-C libraries was assessed by high-sensitivity DNA ScreenTape Analysis (Agilent #5067-5584) on an Agilent 2200 Tapestation.
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bioRxiv - Microbiology 2024Quote: ... An Agilent 1200 Series HPLC system was used with a refraction index detector (RID) at 50°C (Agilent, Santa Clara, CA, USA).
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bioRxiv - Developmental Biology 2020Quote: ... sections were permeabilized with 0.25% TritonX-100 in PBS and autoclaved (121°C, 10 min) with Target Retrieval Solution (Agilent Technologies, Santa Clara, California). Sections were incubated with Blocking One Histo (Nacalai Tesque Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was harvested by centrifugation at 1500 rcf for 4 minutes at 23°C and filtered using a Captiva 96 well 0.2 μm filter plate (Agilent Technologies, Santa Clara, CA) prior to titer measurement by RP-UHPLC.
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bioRxiv - Physiology 2021Quote: Oxygen consumption was measured at 37°C from isolated mouse and human islets after overnight recovery using a Seahorse XF24 analyzer (Agilent, Santa Clara, CA). Islets were seeded at a density of 75 islets/well ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Microbiology 2020Quote: ... the epitopes were unmasked by heating at 95°C for 30 min prior to detection on a Dako OMNIS™ automate (Agilent Technologies, USA). When indicated ...
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bioRxiv - Biochemistry 2019Quote: ... and chromatographic separation was performed at +40 °C using a Pursuit pentafluorophenyl (PFP) column (150 x 2.1 mm, 3.5 µm; Agilent technologies, Santa Clara, CA, USA) connected to a guard column (Pursuit PFP) ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 16 h at 4 °C and purified by HPLC on a Zorbax C3 column with dimensions of 21 × 150 mm (Agilent, Santa Clara, CA) using a gradient of 20-90 % acetonitrile in water with 0.1 % trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2022Quote: Analysis of mitochondrial functions in HAP 1 WT and HAP 1 Δ(c+δ) cells was performed on Seahorse XFe24 (Agilent Technologies, USA) as described previously (Nichols et al. ...
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bioRxiv - Bioengineering 2022Quote: ... and the plates were incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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bioRxiv - Neuroscience 2022Quote: ... and the plate was incubated at 37°C for 30 minutes before reading at 562 nm on a Synergy plate reader (Agilent, Santa Clara, CA). Using the resulting protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the sections were incubated in a humidified chamber (overnight 4°C) in a peroxidase conjugated immunoglobulin against UEA (DAKO, P289; 1:50). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Synthetic Biology 2022Quote: ... the oxidation of NADH was followed at 340 nm and 30 °C on a Cary 60 UV-Vis photospectrometer (Agilent, Santa Clara, USA) in quartz cuvettes with a pathlength of 10 mm (Hellma Optik GmbH ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then optical density at 600 nm (OD600) was monitored with a Biotek Synergy H1M at 30°C for 48 hours with fast continuous shaking (Agilent, Santa Clara, CA).
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bioRxiv - Plant Biology 2024Quote: ... We used a Zorbax SB-C18 5 μm 4.6 x 250 mm column at 35°C on a 1260 Infinity HPLC instrument with a fluorescence detector (Agilent Technologies, Santa Clara CA) using an excitation wavelength of 340 nm and emission wavelength of 510 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
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bioRxiv - Bioengineering 2024Quote: ... and the plate was incubated at 37 °C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 48 hours (Fig ...
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bioRxiv - Biochemistry 2024Quote: Spectra of dark-adapted AsLOV2 and NcVVD variants were acquired at 22°C on a diode-array absorbance spectrophotometer (Agilent Technologies, model 8453) equipped with Peltier temperature control (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
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bioRxiv - Bioengineering 2024Quote: ... and the plates were incubated at 37°C and 5% CO2 in a BioSpa live cell analysis system (Agilent Technologies, Santa Clara, CA) for 45 hours.
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... and incubated overnight in a humid chamber at 4°C with rabbit anti-glial fibrillary acidic protein (GFAP) antibody (1:500, Z0334, DAKO, Agilent, Glostrup, Denmark), rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Synthetic Biology 2023Quote: ... and left to grow at 30 °C and 600 rpm until all cultures reached OD600 above 0.3 measured in a Biotek Synergy H4 Multimode (Agilent, Santa Clara, CA, USA) microplate reader ...
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bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent, Santa Clara, CA) with glucose (10 mM) ...
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bioRxiv - Cell Biology 2023Quote: ... the fibers were placed in an incubator at 37 °C with no CO2 and the medium was replaced with XF Base media (Agilent; Santa Clara, CA), with sodium pyruvate (1 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: Enzymes were assayed at 25 °C by recording the change in absorbance at 340 nm for 300 seconds on a Cary 100 Bio (Agilent, Santa Clara, CA) in 50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated overnight at 4°C with the respective primary antibodies: (i) polyclonal rabbit anti-GFAP (1:2000; Dako, Cat. No. Z0334); (ii ...
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bioRxiv - Immunology 2024Quote: ... Slides were baked in an oven at 70°C for 60 minutes and then loaded onto an automated stainer (Leica Bond III for CD4 staining and Dako Omnis (Dako / Agilent) for BCL6 staining ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by overnight incubation at 4 °C with α-α-SMA (1:400, mouse monoclonal, clone 1 A4, DAKO Agilent Technologies, Santa Clara, USA) or α-CD68 (1:2000 ...
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bioRxiv - Systems Biology 2019Quote: ... 20% of the total mixture was incubated at 37°C with PNGaseF and fractionated into 16 high pH reversed phase fractions using a 1260 Infinity II HPLC (Agilent Technologies, Santa Clara, CA) with a 4.6 × 150 mm XBridge C18 column and a 30 minute gradient (mobile phase A ...
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bioRxiv - Molecular Biology 2019Quote: ... of each lipid class were prepared by acid-catalyzed transesterification at 50°C for 16 hours (Christie, 1973) before quantified by a Agilent 7890B gas chromatograph with flame ionization detector (Agilent Technologies, Santa Clara, CA).