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Citations for Agilent :
1251 - 1300 of 1386 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... An Agilent 1200 Series HPLC system was used with a refraction index detector at 50°C (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice and incubated for 1 h at 37°C in the non-CO2 chamber with 180 μl of the assay medium (Agilent; 103575) supplemented with L-glutamine (2 mM ...
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bioRxiv - Cell Biology 2022Quote: ... treated cells were then equilibrated in seahorse XF base medium supplemented with 1g/L glucose at 37°C in non-CO2 incubator for 1 hour prior to analysis using a Seahorse XF24 Extracellular Flux Analyzer (Seahorse Bioscience).
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bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
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bioRxiv - Physiology 2024Quote: Fuel preference of CRISPR/Cas9 Pparγ edited cells (γY4KO) and mock-edited cells (C) was measured using Seahorse Mito-Fuel Flex test (Agilent Technologies), in assay medium as above and according to the manufacturer protocol ...
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bioRxiv - Biophysics 2024Quote: In vitro NMR experiments were carried out at 25 °C on an 800 MHz Agilent spectrometer equipped with a cryogenic probe (Agilent Technologies). The buffer used in all NMR titration assay was 50 mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were grown at 30 °C in 96-well plates in an BioTek Epoch 2 shaking incubator (Agilent, Santa Clara, CA) for 72 hours.
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bioRxiv - Microbiology 2024Quote: ... The plate was sealed with a gas permeable seal (Azenta Life Sciences 4ti-0516/96*) and incubated at 37°C and 237 cpm for indicated times in a Synergy H1 plate reader (Agilent BioTek). OD600nm was measured every 10 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity - Waters, USA) on a C-8 column (Zorbax Eclipse XBD - Agilent, USA) at a flow rate of 0.6 mL/min and eluted off the column using a hydrophobicity gradient ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 with Dako Target Retrieval Solution (Agilent, USA), in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: A total of 10 µg/ml rHA were coated on an ELISA plate at 4 °C ON and afterwards incubated with rabbit anti-HA antibody (1:2000 dilution) (Dako, A0001) 2h at RT ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Biophysics 2023Quote: ... Cuvettes were then inserted into a preheated (50°C) Cary 3500 UV−Vis spectrophotometer controlled by an Agilent multizone Peltier temperature controller (Agilent Technologies). The samples were cooled to 1°C at a rate of 1°C/min while measuring the absorbance at 600 nm.
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Immunology 2022Quote: ... for 120 s and separated by a C-18 column (Zorbax 300SB 2.1 × 50 mm, 3.5 μm particle diameter, Agilent, Santa Clara, CA). For LC ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
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bioRxiv - Immunology 2022Quote: ... the tissue sections for IMC were heat pre-treated using a target retrieval solution for 30 min at 95°C (K8000, EnVision flex target retrieval solution, pH9 (Agilent Technologies). Slides were cooled down for 20 min at room temperature before rinsing with water and PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated at 37°C in a non-CO2 incubator for 1 hour and subsequently loaded into the Seahorse XFe96 Analyzer (Agilent Technologies, Seahorse Bioscience) when prompted ...
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bioRxiv - Genomics 2023Quote: The fragment size distribution and concentration of the final PacBio and Dovetail Hi-C libraries were assessed using the TapeStation (Agilent Technologies) and the Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor xenograft slides were incubated at 60 °C for 2 h followed by antigen retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Molecular Biology 2023Quote: ... desorption was performed at 250°C for 1’ (splitless mode) in the injection port of a 6890 N gas chromatograph (Agilent Technologies) coupled to a 5975B mass spectrometer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... were constructed from pcDNA3.1+-C-(K)-DYK-EBP plasmids using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, #210519) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: All absorption and emission measurements for both labeled A2AAR and Rec were carried out at 25 °C on a Synergy H4 (Agilent BioTek) plate reader with Take3 Microvolume Plates ...
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bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Neuroscience 2023Quote: ... for 60 min and incubated overnight at 4°C with primary antibodies diluted in DAKO Antibody Diluent (Agilent, cat. #S080983-2). Sections were washed in PBS (3 x 5 min ...
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bioRxiv - Neuroscience 2023Quote: Vibratome cut (free floating) tissue was heated to 70°C in 1x Daco Target Retrieval solution (DakoTarget Retrieval Solution, pH 6.1, Dako, code. S2367) for 15 min and left to cool at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Genetics 2024Quote: The purified RNAs in the 96-well PCR plate (Axygen Scientific, PCR-96-FS-C) were transferred to a 0.3 mL 96-well plate (Agilent, 5043-9313) for hydrolysis in a 40 µL of an enzyme cocktail containing 10 U benzonase (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... sections were deparaffinized, dehydrated and subjected to antigen retrieval (20 min incubation at 98 °C in citrate buffer, pH 6 (Agilent Dako) for CD8 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was fragmented and hybridized to a microarray for 17 h while rotating at 10 rpm at 65 °C in a hybridization oven (Agilent Technologies). The hybridized microarray was washed and scanned according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Log-phage cultures were inoculated into specified media and monitored for growth rate every 15 min at OD600 for 24 or 72 hours at 37°C under static conditions using BioTek Synergy H1 (Agilent Technologies).
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bioRxiv - Microbiology 2021Quote: ... The test tubes were incubated for 18-24 hours at 37 °C after which turbidity was observed and optical density recorded using “Cary Series UV/Vis” (Agilent Technologies, Germany). The lowest concentration that completely inhibited the growth of the test organisms as observed by the no growth when the concentration with the least optical density is plated out on nutrient agar was reported as the MIC ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22 mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was started with different concentrations of 3-butenoic acid and the consumption of NADPH was followed at 30 °C and 340 nm (Δε=6.22mM-1 cm-1) with a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Germany).
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored at −80°C after verifying concentration and quality by spectrophotometer (Nanodrop, Thermo scientifics) and a Bioanalyzer (RNA 6000 Nano Kit, Agilent Technologies Inc). We produced libraries for these 21 individuals using the TruSeq RNA Library Prep Kit v2 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... blocked using 10% Normal Goat Serum (NGS) and incubated overnight with the following primary antibodies in 5% NGS overnight at 4°C: rabbit anti-GFAP (DAKO; #Z0334;1:1000), rat anti-L1-CAM (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... at 37⁰C (1:50 protein/trypsin ratio) and cleaned up with a C18 column (OMIX C18, Agilent, Santa Clara, CA, USA). Nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS ...
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bioRxiv - Cancer Biology 2020Quote: ... slides with PCa tissue samples (n=144) were incubated at 60°C for 2 hours followed by target retrieval in a PT Link instrument (Agilent DAKO, PT200) using EnVision FLEX Target Retrieval Solution ...
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bioRxiv - Molecular Biology 2022Quote: ... they were first permeabilized using an acetone freeze step for 7 min at -20° C before staining with guinea-pig anti-insulin (Dako, Carpinteria, CA), Alexa Fluor 488 or 647 goat anti-guinea-pig (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
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bioRxiv - Physiology 2019Quote: ... for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent: anti-Insulin (DAKO, 1:1000), anti-BRD4 (Bethyl laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... for 4 hours at 4°C with rotation and processed using the Agilent Bravo liquid handling system (Agilent Technologies, Santa Clara, CA). Beads were washed twice with 0.1% NP-40 in Tris-buffered saline (50 mM Tris-HCl with 150mM NaCl ...