Labshake search
Citations for Agilent :
1101 - 1150 of 1386 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2020Quote: ... followed by a final elongation step at 72 °C for 5 min (SureCycler 8000, Agilent technologies, Santa Clara, CA, USA). In order to amplify different transcripts via nested PCR from the renin gene ...
-
bioRxiv - Immunology 2019Quote: ... incubated overnight at 4 °C with polyclonal guinea pig anti-insulin antibody (1:100 in 1% BSA in DPBS, DAKO) followed by 1 h room temperature incubation either with Alexa Fluor® 488 AffiniPure donkey anti-guinea pig or with DyLight™ 594 AffiniPure donkey anti-guinea pig secondary antibody (1:100 in 1% BSA in DPBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... France) Citrate buffer pH6 was used for antigen retrieval 20min à 96°C (Target Retrieval so-lution low pH, Dako) and DAB and Hematoxylin staining were revealed using ImPath detection kit (DAB OB Sensitive Detection Kit ...
-
bioRxiv - Biochemistry 2019Quote: Dried peptides were reconstituted in 15 μl of 0.1% formic acid in water and 5 μl of sample was applied to an ACQUITY UPLC C18 reverse phase column (Waters) maintained at 40°C using an Infinity 1290 autosampler and HPLC (Agilent). Peptides were eluted at 0.1 ml/min using a varying mobile phase gradient from 95% phase A (FA/H2O 0.1/99.9 ...
-
bioRxiv - Immunology 2019Quote: ... The density of HLA-C per bead was calculated using QIFIKIT calibration beads according to the manufacturer’s instructions (Dako, K0078). Calibration beads contained five populations of beads conjugated to different numbers of mouse immunoglobulin ...
-
bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a nitrocellulose membrane before probing using a primary anti IL-36γ antibody (Biotechne (R&D) UK) overnight at 4°C and a secondary goat antibody (Dako) for 1 hour at room temperature to visualise any protein cleavage.
-
bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...
-
bioRxiv - Cancer Biology 2021Quote: ... Slides were de-coverslipped with 1 min of agitation in TBST and subjected to heat-mediated antigen retrieval in 1x pH 6.0 citrate buffer (Biogenex Laboratories, HK0809K) for 20 min at 95°C followed by blocking of endogenous peroxidase activity (Dako, S2003 ...
-
bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100; autosampler: HiP sampler G1367A, T=4°C, 10 μL injection; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
-
bioRxiv - Microbiology 2021Quote: ... The concentration of protein was determined using a Poroshell 120 SB-Aq column (2.1 x 50 mm, 1.9μm) operated at 1.0 mL/min and 70°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
-
bioRxiv - Plant Biology 2021Quote: ... The Zorbax Eclipse Plus C-18 column Rapid Resolution HT (3 X 100 mm, 1.8 μm, 600 bar, Agilent, USA) was kept at 25°C and the elution was performed with acetonitrile (Optima ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 30 min or with 0.4 µg/ml anti-human cath-D mouse monoclonal antibody (clone C-5) for 20 min after heat-induced antigen retrieval with the PTLink pre-treatment (Dako) and the High pH Buffer (Dako ...
-
bioRxiv - Cell Biology 2020Quote: ... the formalin-fixed and paraffin-embedded bronchial organoid samples were treated with pH 6.0 citrate buffer for 30 sec at 125°C in a pressure cooker (Dako Japan) as antigen retrieval ...
-
bioRxiv - Immunology 2022Quote: ... The following antibodies were incubated in 0.1% Triton with 2% goat serum in PBS at 4°C overnight: rabbit anti-GFAP (1: 1000, DAKO, Z0334), rabbit anti-IBA-1 (1 ...
-
bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... The SEC24A B- and C-site mutants were generated on pcDNA3.1-SmBiT-SEC24A using QuickChange site-directed mutagenesis (Agilent Technologies).
-
bioRxiv - Neuroscience 2020Quote: ... prior to target retrieval at 97°C for 30 minutes with EnVision FLEX TRS Low pH target retrieval solution (citrate buffer, pH 6.1; K8005, Agilent DAKO), followed by a wash step with wash buffer (GC807 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
-
bioRxiv - Neuroscience 2021Quote: ... 3 μm paraffin-embedded tissue sections were either dewaxed and subjected to antigen retrieval treatment with Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent) or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005 ...
-
bioRxiv - Cancer Biology 2021Quote: ... slides were de-waxed and antigen retrieval was performed in a pressure cooker at 125°C for 3 min employing an Antigen Retrieval solution (Dako, pH6 for EZH2 ...
-
bioRxiv - Developmental Biology 2022Quote: PSM cells were dissociated with accutase for 5 min at 37 °C during the most efficient day of differentiation and re-seeded into fibronectin-coated Seahorse plates (Agilent) at a density of 7.27 × 105 cells per cm2 in 100 μL of Seahorse XF DMEM (Agilent ...
-
bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
-
bioRxiv - Genetics 2019Quote: ... Final Hi-C libraries were quantified using Qubit dsDNA HS assay kit and a DNA HS kit on a 2100 Bioanalyzer (Agilent). Libraries were first pooled and shallow sequenced on an Illumina MiSeq (2×84bp paired-end ...
-
bioRxiv - Cell Biology 2020Quote: ... Prior to immunohistochemistry antigen retrieval was performed using Tris-EDTA buffer pH 9 for 20 min at 97°C using a PT Link (Dako – Agilent). Quenching of endogenous peroxidase was performed by a 10 min incubation with Peroxidase-Blocking Solution (Dako REAL S2023) ...
-
bioRxiv - Immunology 2020Quote: RNA was isolated form peripheral blood of febrile controls and MIS-C patients and assessed for quality with a bioanalyzer (Agilent). Sequencing of the TCR genes was performed using the QIAseq Immune Repertoire RNA Library Kit (Qiagen ...
-
bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
-
bioRxiv - Plant Biology 2021Quote: ... and cloned into pVp16-Dest vector for IPTG (isopropyl-β-thiogalactopyranoside)-induced expression (3 h at 37°C) in E.coli strain ArcticExpress (Agilent). After sonication ...
-
bioRxiv - Developmental Biology 2019Quote: ... for one hour at room temperature and incubated overnight at 4°C with 1:200 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
-
bioRxiv - Genomics 2019Quote: ... Probes were hybridised to chicken metaphase slides for 17 hours at 37°C and to duck metaphases for 48H in the Hybridizer (Dako). Chromosomes were counterstained with DAPI in antifade solution (Vectashield with DAPI ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1µg of Hi-C library per sample was mixed with 5 μl of SureSelect XT HS and XT Low Input Blocker Mix (Agilent). Samples were denatured at 95°C for 5 minutes and pre-hybridized for 10 minutes at 65°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
-
bioRxiv - Developmental Biology 2021Quote: ... Final Hi-C libraries were quantified using Qubit dsDNA HS assay kit and a DNA HS kit on a 2100 Bioanalyzer (Agilent). Libraries were first shallow sequenced on an Illumina MiSeq (2×84bp paired-end ...
-
bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
-
bioRxiv - Cancer Biology 2021Quote: ... Antigen unmasking was carried out in a DAKO PT-Link for 20 minutes at 97°C using EnVision FLEX buffer of appropriate pH for each antibody (Agilent). Then ...
-
bioRxiv - Biophysics 2019Quote: ... construct by deleting 14aa from near the C-terminus of PRC1 isoform 1 (aa:1-620) using Quikchange mutagenesis (Agilent). The PRC1 isoform 2 gene was then in a pET-DUET plasmid containing an N-terminal histidine tag followed by a Tobacco Etch Virus (TEV ...
-
bioRxiv - Biophysics 2021Quote: ... sections were fixed for 10 min in cold acetone (−20 °C) and rinsed in TBS (Agilent, Santa Clara, CA, USA). For staining of MΦs ...
-
bioRxiv - Biochemistry 2022Quote: ... Assay of formate:NAD+ oxidoreductase activity was conducted as described in the previous section at 37 °C with a BioTek Synergy Neo2 plate reader (Agilent) in buffer composed of 10 mM MES pH 6.0 ...
-
bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
-
bioRxiv - Cell Biology 2022Quote: ... Sections were then heated to 97°C for 20 minutes in a high pH target retrieval solution (TRS) (K8004, Agilent) for heat-induced antigen retrieval (HIER) ...
-
bioRxiv - Microbiology 2022Quote: ... 30 sec at 60°C (steps 3 and 4 were repeated 40 times) was done with the AriaMX real-time PCR System (Agilent).
-
bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
-
Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... were hydrated overnight at 37°C in a non-CO2 incubator with 200 µl/well of the Seahorse XF calibrant (Agilent). The day of the assay ...
-
bioRxiv - Neuroscience 2022Quote: ... Retinal disks were incubated for 30 minutes at 37°C prior to running the assay using a Seahorse XF96 Analyzer (Agilent). During the assay ...
-
bioRxiv - Immunology 2022Quote: ... and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse, Agilent) for measuring the extracellular acidification rate (ECAR ...
-
bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
-
bioRxiv - Cancer Biology 2022Quote: The sensor cartridge plate was hydrated with sterile distilled water overnight at 37°C in a CO2-free incubator and was replaced with a calibrant solution (Agilent) for 1 hour ...
-
bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
-
bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...