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Citations for Agilent :
1051 - 1100 of 1284 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and then with the primary (Supplementary Table 2) (overnight 4°C) and the secondary (HRP-conjugated anti-mouse or -rabbit, DAKO) (2h ...
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bioRxiv - Developmental Biology 2021Quote: ... Final Hi-C libraries were quantified using Qubit dsDNA HS assay kit and a DNA HS kit on a 2100 Bioanalyzer (Agilent). Libraries were first shallow sequenced on an Illumina MiSeq (2×84bp paired-end ...
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... Antigen unmasking was carried out in a DAKO PT-Link for 20 minutes at 97°C using EnVision FLEX buffer of appropriate pH for each antibody (Agilent). Then ...
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bioRxiv - Biophysics 2019Quote: ... construct by deleting 14aa from near the C-terminus of PRC1 isoform 1 (aa:1-620) using Quikchange mutagenesis (Agilent). The PRC1 isoform 2 gene was then in a pET-DUET plasmid containing an N-terminal histidine tag followed by a Tobacco Etch Virus (TEV ...
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bioRxiv - Biophysics 2021Quote: ... sections were fixed for 10 min in cold acetone (−20 °C) and rinsed in TBS (Agilent, Santa Clara, CA, USA). For staining of MΦs ...
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bioRxiv - Biochemistry 2022Quote: ... Assay of formate:NAD+ oxidoreductase activity was conducted as described in the previous section at 37 °C with a BioTek Synergy Neo2 plate reader (Agilent) in buffer composed of 10 mM MES pH 6.0 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were then heated to 97°C for 20 minutes in a high pH target retrieval solution (TRS) (K8004, Agilent) for heat-induced antigen retrieval (HIER) ...
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bioRxiv - Microbiology 2022Quote: ... 30 sec at 60°C (steps 3 and 4 were repeated 40 times) was done with the AriaMX real-time PCR System (Agilent).
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bioRxiv - Microbiology 2022Quote: 2 μl of extracted metabolite samples were separated on a Cogent Diamond Hydride Type C Column of 1200 liquid chromatography (Agilent) which was coupled to an Agilent Accurate-Mass 6220 Time-of-Flight spectrometer ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... were hydrated overnight at 37°C in a non-CO2 incubator with 200 µl/well of the Seahorse XF calibrant (Agilent). The day of the assay ...
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bioRxiv - Neuroscience 2022Quote: ... Retinal disks were incubated for 30 minutes at 37°C prior to running the assay using a Seahorse XF96 Analyzer (Agilent). During the assay ...
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bioRxiv - Immunology 2022Quote: ... and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse, Agilent) for measuring the extracellular acidification rate (ECAR ...
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bioRxiv - Physiology 2022Quote: ... sections were dewaxed and epitope retrieval was performed using Citrate buffer pH6 for α-SMA and Ter119 or Tris- EDTA buffer pH9 for HMOX1 in all cases for 20 min at 97°C using a PT Link (Dako, Agilent) or with ER2 buffer (AR9640 ...
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bioRxiv - Cancer Biology 2022Quote: The sensor cartridge plate was hydrated with sterile distilled water overnight at 37°C in a CO2-free incubator and was replaced with a calibrant solution (Agilent) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 hour before being placed into a Seahorse XFe24 Analyzer (Agilent). For oxygen consumption rate (OCR ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... The HPLC was equipped with a C-18 column (4.6 mm by 150 mm column, Zorbax Eclipse XDB-C18, Agilent Technologies) and inline radio-detector (FlowStar LB 513 ...
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Microbiology 2023Quote: Pooled gradient fractions were diluted 1:2 with ice-cold PBS and tumbled overnight at 4°C with 50 µl Strataclean resin (Agilent). Beads were pelleted at 600 RCF for 5 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Shimadzu LC-10AT; autosampler: HiP sampler G1367A, T = 4°C, 10 µL injection; flow rate: 1 mL/min; column: Agilent Zorbax Eclipse XDB-C18 80Å ...
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bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
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bioRxiv - Cell Biology 2022Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W). Helium was used as the carrier gas ...
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bioRxiv - Cell Biology 2023Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W) and helium was used as the carrier gas ...
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bioRxiv - Biophysics 2023Quote: ... emission 353 nm) of 25 μM PrP was acquired upon CuCl2 titration at 25°C in a Cary Eclipse Fluorescence Spectrophotometer (Agilent) with slits of 5 nm (excitation ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and kept at 4°C overnight with primary antibodies (chicken anti-GFP, Aves Labs, 1:4000; rabbit anti-GFAP, Agilent Pathology Solutions ...
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bioRxiv - Bioengineering 2023Quote: ... read height of 7 mm and at 37 °C with excitation at 534-554 nm wavelength using a Cytation5 (Agilent). All values were subtracted by a control with only 8FN microgels and no cells.
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bioRxiv - Bioengineering 2023Quote: ... read height of 7 mm and at 37 °C with excitation at 315-335 nm wavelength using a Cytation5 (Agilent). All values were subtracted by a control with only 8FN microgels and no cells.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence was analyzed in a thermostat controlled (37°C) and magnetically-stirred Cary Eclipse fluorescence spectrophotometer (Agilent Technologies, Milano, Italy) or using a confocal microscope (LSM Carl Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatographic separation was carried out at 40°C using an Agilent 1260 Infinity II series UPLC (Agilent, Santa Clara, USA) over a 4 minute gradient elution (KNOWNS_AM190319.m ...
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bioRxiv - Plant Biology 2023Quote: ... Enzyme activity was determined by the consumption of NADH at 340nm using a diode array spectrophotometer at 25°C (Agilent).
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bioRxiv - Microbiology 2023Quote: ... a mutant version of each of the hsdR1 had a unique BamHI site inserted by site directed mutagenesis of a C at position 1033 of hsdR1 to a G using oligonucleotide directed mutagenesis (5’-CATCAGAGACTTTTTTAGCGGATCCAACCTAAACAAAAAGAC-3’) (Quick-Change Lightning. Agilent). The mutated site is shown in bold and italics and the generated BamHI site is underlined ...
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bioRxiv - Genetics 2023Quote: ... Membranes were labelled with primary antibody for 1 hour at room temperature or overnight at 4°C followed by incubation with HRP-conjugated secondary antibodies (Dako). Membranes were developed using the Western Lightning ECL system (Perkin Elmer) ...
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinization and antigen retrieval was performed in the low pH EnVision FLEX Target Retrieval Solution at 98 °C for 24 minutes in the DAKO PT link from Agilent. Antibodies were diluted in DaVinci Green Diluent (Biocare ...
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bioRxiv - Genomics 2023Quote: ... 500-1000ng of Hi-C library were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: Log phase cultures of cells grown without inducer were diluted to an OD600 of 0.05 and grown in 96-well plates for 24 h at 30°C with constant shaking on a BioTek Cytation 1 plate reader (Agilent), measuring the absorbance at 600 nm every 30 min for three technical replicates for each strain ...
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bioRxiv - Physiology 2023Quote: ... Some 20 μl of re-dissolved potion (b) and portion (c) solutions were then loaded into injection vials an injection vial (cat. 9301-0978, Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... OD600 measurements were performed at 30°C every 15 min until a plateau was reached in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent).
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bioRxiv - Genomics 2023Quote: ... the slide was baked at 60 °C for 60 minutes and then processed through the Dako Progressive H&E protocol (Agilent) and using Dako Harris Hematoxylin ...
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bioRxiv - Microbiology 2023Quote: ... Samples were injected in splitless mode (injector temperature 300 °C) and separated on a DB5 column (60 m × 0.25 mm × 0.25 μm; Agilent, Vienna, Austria) with 1.2 ml He min−1 as the carrier gas (GC program ...
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bioRxiv - Microbiology 2023Quote: ... primer annealing and extension at 58°C for 30 s followed by dissociation step in a thermal cycler (Stratagene Mx3005P). Finally ...
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bioRxiv - Molecular Biology 2023Quote: Size Exclusion Chromatography in line with Multi Angle Laser Light Scattering (SEC-MALLS) experiments for absolute mass determination were conducted at 25°C with a BioSEC-3 column (Agilent) equilibrated in 20 mM Na-phosphate pH 7.0 ...
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bioRxiv - Molecular Biology 2023Quote: TEV eluates (about 650-700 µl) were partially digested for 5 min at 37 °C with 25 µl of RNase-IT (Agilent) diluted to 1:50 in TNM100 buffer and the reactions were stopped using 0.4 g guanidine hydrochloride ...
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bioRxiv - Cell Biology 2023Quote: ... Kidneys were equilibrated at 37°C for 1 h in a non-CO2 incubator and then assayed on a Seahorse XFe24 Analyzer (Agilent) following a 12-min equilibration period ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence anisotropy titrations with FITC-labeled Nbs and CtRoco were performed at 25°C using a Cary Eclipse spectrofluorometer (Agilent) equipped with polarizers and temperature control ...
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bioRxiv - Microbiology 2023Quote: ... Culture plates were incubated at 37°C for 22 hrs and kept anaerobic during the OD600 measurement in a Synergy 2 Plate Reader (Biotek Agilent Technologies Deutschland GmbH ...