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Citations for Agilent :
751 - 800 of 1386 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA). A solution of fatty acid methyl esters (C8 to C30 ...
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bioRxiv - Physiology 2022Quote: ... Extracted collagen was derivatized for analysis of deuterium enrichment of alanine using Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA). To determine the precursor pool enrichment ...
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bioRxiv - Immunology 2022Quote: ... GC-MS analysis of both TBDMS derivatives and FAMEs were conducted on an Agilent 7890/5977b GC/MSD equipped with a DB-5MS+DG (30 m x 250 μm x 0.25 μm) capillary column (Agilent J&W, Santa Clara, CA, USA) was used ...
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bioRxiv - Physiology 2020Quote: ... αPg and βPg were analyzed using proprietary in-house assays referred to as Dried Urine Testing for Comprehensive Hormones (DUTCH) on the Agilent 7890/7000B GC–MS/MS (Agilent Technologies, Santa Clara, CA, USA). The equivalent of approximately 600 μl of urine was extracted from the filter paper using acetate buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The concentration of the ester in the n-hexadecane phase was quantified using a gas chromatography-mass spectrometry (GC-MS, Agilent Technologies 6890N, Santa Clara, CA) equipped with an HP-5 column (60m×0.25 mm ...
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bioRxiv - Immunology 2022Quote: ... GC-MS analysis of both TBDMS derivatives and FAMEs were conducted on an Agilent 7890/5977b GC/MSD equipped with a DB-5MS+DG (30 m x 250 µm x 0.25 µm) capillary column (Agilent J&W, Santa Clara, CA, USA) was used ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at room temperature for 1 hour and analyzed using gas chromatography-mass spectrometry (6890N GC, coupled to 5873N Mass Selective Detector; Agilent Technologies, Santa Clara, CA, USA). Statistical significance was assessed by mixed linear model with pBMI ...
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bioRxiv - Genetics 2023Quote: ... The SPME fiber was then inserted into the injector of an Agilent 7890 GC coupled with a 5975 MS detector (Agilent Technologies, Palo Alto, CA, USA) for 15 min at 250 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The GC/MS analysis was carried out using an Agilent 7890A gas chromatograph coupled to an Agilent 5975C mass detector (Agilent Technologies, Palo Alto, CA, USA). Mass spectra were recorded in electron impact (EI ...
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bioRxiv - Immunology 2023Quote: ... an Agilent 5975C GC/MS equipped with a DB-5MS+DG (30 m x 250 µm x 0.25 µm) capillary column (Agilent J&W, Santa Clara, CA, USA) was used ...
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bioRxiv - Microbiology 2023Quote: Headspace CO2 of fungal incubations (1 mL) were measured for CO2 concentration on a Hewlett Packard HP 5890 Series II GC (Agilent Technologies, Inc, Santa Clara, USA) coupled with a Thermal conductivity detector (TCD) ...
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Phylogeny-metabolism dual-directed single-cell genomics for dissecting and mining ecosystem functionbioRxiv - Microbiology 2023Quote: ... GC and GC-MS analyses were performed on an Agilent 7980A gas chromatograph equipped with an Agilent 5975C mass selective detector (Agilent Technologies, Little Falls, DE, USA) and a HP-INNOWAX capillary column (30 m × 0.25mm × 0.25µm ...
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bioRxiv - Physiology 2024Quote: ... an Agilent 5975C GC/MS equipped with a DB-5MS+DG (30 m x 250 µm x 0.25 µm) capillary column (Agilent J&W, Santa Clara, CA, USA) was used ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting FA methyl esters were extracted with hexane and subjected to Agilent 7890A-5975C GC-MS instrument with an Agilent 19091N-205 capillary column (Agilent Technologies, Santa Clara, CA, USA). Peak alignment ...
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bioRxiv - Biophysics 2021Quote: ... A reverse phase C-18 column (4.6 x 150 mm, Agilent Eclipse XDB- C-18) was used to separate the reaction mixture containing nucleotides by an HPLC using buffer A (100 mM KH2PO4 ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Physiology 2020Quote: ... Slides were blocked with 20% FCS in PBS and incubated overnight with 1/10 anti-insulin (Dako) and 1/500 anti-Ki67 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... dissociation curve 60°C-95°C as pre-defined by the Mx3005P thermal cycler (Agilent technologies). In vitro transcribed CHIKV ICRES RNA was reverse transcribed and a cDNA dilution series employed as a standard to quantify copy numbers in the respective samples.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected into the GC (Sigma-Aldrich 5973 Network mass selective detector attached to an Agilent 6890 gas chromatograph (Agilent Technologies, Santa Clara, CA, USA), equipped with a capillary HP5-MS column (Agilent Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The SPME fibre was retracted and immediately inserted into the inset of a Gas Chromatography-mass spectrometry (GC-MS) system (Agilent 7890B fitted with MS 5977A unit) for desorption at 260°C in split mode (split ratio 100:1) ...
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bioRxiv - Microbiology 2022Quote: ... derivatization followed by gas chromatography – electron-ionization-time-of-flight mass spectrometry (GC-EI-TOFMS) in split mode (split 1:50, Agilent straight split liners with glass wool) was used15 ...
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bioRxiv - Physiology 2024Quote: ... To analyze the deuterium enrichment of alanine in the protein fractions we used Gas Chromatography-Mass Spectroscopy (7890A GC-Agilent 5975C MS, Agilent, Santa Clara, CA)52,53.
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bioRxiv - Evolutionary Biology 2023Quote: ... using an Agilent model 5977 mass-selective detector connected to an Agilent GC model 7890B and equipped with an Agilent ALS 7693 autosampler using a HP-5MS fused silica capillary columns (Agilent, 30 m × 0.25 mm, 0.25 µm). Injection was performed in splitless mode (250°C injector temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Individual protein fractions were next derivatized for subsequent analysis of deuterium enrichment in alanine using Gas Chromatography-Mass Spectroscopy (Agilent 7890B GC coupled to Agilent 5977B MS, Agilent, Santa Clara, CA). To assess ribosomal biogenesis total RNA was extracted from ∼10 mg of heart tissue using Qiagen RNeasy Fibrous Tissue Mini Kit (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... Extracts were derivatized using an AccQ•Tag Ultra Derivatization Kit (Waters Corp.) according to the manufacturer’s protocol and analyzed using an HPLC (Agilent) coupled to a mass spectrometer (4500 QTRAP (Sciex) ...
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bioRxiv - Neuroscience 2019Quote: ... of the canonical 307 amino acid human NMNAT2 variant (NCBI ProteinID: NP_055854) cloned in expression vector pCMV Tag-2B (Stratagene). A short linker (17 amino acids ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Amber (TAG) codons were introduced site-specifically in the 2B4 TCR and CD3 plasmid using Quikchange mutagenesis kit (Agilent).
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bioRxiv - Biochemistry 2020Quote: ... An HA tag following the CXCR4 start codon and D20R and E26R mutations were introduced by QuikChange mutagenesis (Agilent).
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reference plasmids containing PA tag sequence were constructed in pBluescript II SK (+) vector (Agilent, Palo Alto, CA, USA). One μg of guide RNA inserted vector and 1.0 μg of reference plasmid were co-transfected into EGRG01 embryonic stem (ES ...
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bioRxiv - Genomics 2021Quote: ... anti-CD117 (clone c-kit, DAKO). The molecular markers of immune panel (CD3 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in DMEM with 10% of FCS and transferred into xCELLigence microplates (E-plate 16, Agilent) at the density of 20 000 cells for real-time analysis of cell adhesion and growth ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated from 25°C to 95°C in a Mx3005P real-time PCR instrument (Stratagene). Fluorescence was monitored with excitation and emission filters set to 465 and 590 nm ...
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bioRxiv - Systems Biology 2021Quote: ... One microliter of the derivatized sample was injected in splitless mode using an autosampler (VCTS, Gerstel™, Linthicum, MD, USA) into a GC-MS system consisting of an Agilent© 7890 B gas chromatograph (Agilent Technologies, Palo Alto, CA, USA) with Pegasus ® 4D ToF-MS instrument (LECO Corp. ...
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bioRxiv - Microbiology 2019Quote: The samples and standards were then placed in a GC-MS (GC-MS-QP2010 Ultra fitted with an AOC-20s Shimadzu auto sampler and a Shimadzu AOC-20i auto injector) with a polar column (Agilent J&W GC, 30m, 0.250 diam (mm), film 0.25 (μm ...
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bioRxiv - Genetics 2023Quote: ... The system was equipped with a fused silica column (DB-5MS ultra inert; 30 m x 250 μm x 0.25 μm; Agilent J&W GC columns, Santa Clara, CA, USA) with helium used as a carrier gas under a constant flow of 1.8 ml/min ...
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bioRxiv - Plant Biology 2023Quote: ... Lipid bands were scratched from the plates and their fatty acids extracted (fatty acid methyl esters FAMEs) and quantified by GC-MS (Agilent 7890 A and MSD 5975 Agilent EI) as in (39) ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... was fused with a six histidine tag (6xHis) and an AviTag (pET-28a-AviTag-GluN1C0) and expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). Expression of His-AviTag-GluN1(834-863 ...
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bioRxiv - Biochemistry 2019Quote: ... All additional tags were added via Gibson assembly and all mutations and truncations were made via site-directed mutagenesis (Agilent). For rapalog induced motility in cells ...
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bioRxiv - Biochemistry 2020Quote: ... Salmonella typhimurium MsbA (T561C in a C88A/C315A cysteine-less background) with an N-terminal poly-histidine-tag was expressed in BL21-CodonPlus (DE3)-RIPL (Agilent). Expression was induced with 1 mM IPTG for 4 hours at 30°C and membrane proteins were solubilized with 1% DDM and 0.04% sodium cholate in a buffer containing 100 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and pCMVTag 4 expression control plasmid containing the firefly luciferase gene fused with the FLAG tag (luc-FLAG) were obtained from Stratagene. The plasmid 15cxCAT contained the interleukin-2 (IL-2 ...
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bioRxiv - Biochemistry 2022Quote: ... BioID2-tagged variants were generated by introducing a BamHI site between the 8xHis and MBP tags in the 8xHis-MBP-pFastBac1 modified with a CMV promoter vector by Quikchange Lightning site-directed mutagenesis (Agilent) and cloning BioID2 into the BamHI sites using In-Fusion EcoDry cloning according to manufacturer protocols.
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bioRxiv - Biophysics 2022Quote: ... The insertion of an amber codon (TAG) between E715 and V716 in mGluR2 (azi-ECL2) was performed using the QuikChange site-directed mutagenesis kit (Agilent). SNAP-mGluR2 constructs used for calcium imaging had C-terminal FLAG-tag removed by PCR-based deletion using phosphorylated primers ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting DNA fragments were cloned in frame with the FLAG tag using the oligonucleotides that are indicated in Supplementary file 2 into the pESC-URA vector (Agilent), in which the GAL10 and GAL1 promoters were replaced with the Cup1 promoter ...
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bioRxiv - Microbiology 2019Quote: ... a BglII restriction site within the linker-sequence separating the N-terminal mCherry-tag from hGBP1 was eliminated in pmCherry-hGBP1 by Quickchange Site Directed Mutagenesis (Agilent) using the oligomer pair pmCherry-hGBP1DBglII-F and -R (Table S9) ...
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bioRxiv - Cell Biology 2021Quote: ... All additional tags were added via Gibson assembly and all mutations and truncations were made via site-directed mutagenesis (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... 6XHis tag was added to the N-terminus of Upf1 in pGEM-3Zf (+) using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) with oligonucleotides 5-N-His-UPF1 and 5-N-HIS-UPF1-r to yield pGEM3Zf(+)-6XHis-UPF1 ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...