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Citations for Agilent :
751 - 800 of 1284 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were denatured in the presence of the specific probe at 75°C for 5 min and left overnight for hybridization at 37°C in a hybridizer machine (DAKO). The probes used detect copy number alterations in chromosomes 7 and 8 (KBI-20031 ...
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bioRxiv - Plant Biology 2023Quote: ... Temperature-dependent phase transition was detected by measuring the optical disturbance at 800 nm by increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface.
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bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Immunology 2024Quote: ... Quality control was performed by estimating the concentration and measuring the average fragment size of the mRNA target library and sample tag library using the Agilent Tape Station with the High Sensitivity D1000 ScreenTape (Agilent, 5067-5584). The final libraries were diluted to a concentration of 4 nM and multiplexed for Novaseq paired-end sequencing (150 bp ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems, Agilent Technologies). Primers for circadian clock genes were designed using pcrEfficiency (Mallona et al. ...
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bioRxiv - Genetics 2023Quote: ... were fragmented at 60°C for 30min and hybridized to the microarrays for 17h at 65°C in a rotating hybridization oven (Agilent Technologies). After hybridization ...
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bioRxiv - Microbiology 2024Quote: ... 40x 10 sec 95°C and 30 sec 60°C with the Brilliant III Ultra-Fast qRT-PCR master mix (Agilent, 600884) were used according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... were subjected to quantitative PCR under a temperature profile of 95°C for 3 min followed by 40 cycles for 95°C for 5 sec and 58°C for 15 sec using the Stratagene Mx3000P (Agilent Technologies). For each of the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Immunology 2021Quote: The KA and LALA mutations were introduced to the Fc fragment of 2219 by QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) according to the instruction manual ...
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bioRxiv - Biochemistry 2021Quote: ... OPG2-E was generated by cloning the cDNA encoding the E-protein into the same pcDNA5-OPG2 vector using the KpnI and BamHI sites and deleting the stop codon after the OPG2 tag by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies). The N-terminal OPG2-tag of OPG2-ORF6-OPG2 was inserted by site-directed mutagenesis of ORF6-OPG2 using the relevant forward and reverse primers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: All DNA panel and antibody tag libraries were run on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies, cat. no. 5067-4626) to verify complete removal of primer-dimer products ...
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bioRxiv - Biophysics 2020Quote: ... A thrombin site was introduced between the 6xHis-tag and the coiled-coil using the QuikChange Lightning mutagenesis kit (Agilent, Santa Clara, USA). Sequences of the products were confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and H249A were generated from the parent pLVX Kernow C1/p6 ORF1AAAPG-Ha tag-AAAPG IRES zsGreen construct via QuikChange XL site-directed mutagenesis kit (Stratagene, La Jolla, CA). using primers listed in (Supplementary Table 1).
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bioRxiv - Biochemistry 2021Quote: ... at 4°C overnight diluted in antibody diluent (Dako). Negative controls were incubated with antibody diluent only ...
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bioRxiv - Immunology 2021Quote: ... DMEM (Agilent Cat# 102353, pH=7.4 at 37°C), supplemented with 143 mM NaCl and 2mM L-Glutamine for 1 hr ...
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bioRxiv - Bioengineering 2022Quote: ... and a PLgel 5μm MIXED-C column (Agilent Technologies) with chloroform eluent (1 mL/min) ...
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bioRxiv - Bioengineering 2022Quote: ... and a PLgel 5μm MIXED-C column (Agilent Technologies) with chloroform eluent (1 mL/min) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and cooled to 37 °C (Agilent, Catalog #5190-9527). Eighteen replicate transformations of the cloned library were conducted using Agilent’s ElectroTen-Blue cells (Catalog #200159 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 20 min at 97°C (PT-Link, DAKO). Endogenous peroxidase activity was quenched by incubation in peroxidase blocking buffer (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... cytokeratin (DAKO, Cat. M3515; 1:100 at 4 °C), ki67 (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... 66.3°C) in a PCR machine (Agilent SureCycler 8800) followed by 3 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
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bioRxiv - Systems Biology 2021Quote: ... Sample fractions were continuously collected into 96 well plates by an Agilent 1100 Series Micro-FC G1364D micro fraction collector (Agilent Technologies, Germany). For each sample ...
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bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
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bioRxiv - Microbiology 2020Quote: ... sealed with adhesive and incubated for 10 min at 65°C and overnight at 37°C on a FISH-hybridizer (Dako; Agilent Technologies, Santa Clara, CA). The slides were then immersed in a series of baths with SSC buffer at different concentrations of 4× ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Microbiology 2019Quote: ... the microarray was analysed using the Microarray C Scanner (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Zorbax 300 SB-C column from Agilent (Palo Alto, CA) and HALO ES column from Advanced Materials Technology (Wilmington ...
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bioRxiv - Cancer Biology 2022Quote: ... Glyko® Sialidase C™ was from Prozyme (Hayward, CA). All solutions were made with ultrapure Milli-Q water (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... using specific software of data analysis for GC from Agilent (OpenLAB CDS ChemStation v. C.01.10; Agilent Technologies, Barcelona, Spain) and subsequent expert’s revision and confirmation ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were further cleaned by C-18 resin columns (Agilent). (Cleanup and elution of peptides via C-18 column was performed as per the company protocols) ...
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bioRxiv - Cell Biology 2019Quote: OpenLAB CDS ChemStation Edition software version C.01.06 (Agilent Technologies) was used to analyze GC data ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... A C-18 column (2.1 × 100 mm, 1.8μm, Agilent, Inc.) was used to perform the separation ...
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bioRxiv - Biochemistry 2019Quote: ... Glyko® Sialidase C™ was from Prozyme (Hayward, CA). All solutions were made with ultrapure Milli-Q water (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... ICP-MS MassHunter 4.4 software Version C.01.04 from Agilent was used ...
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bioRxiv - Microbiology 2020Quote: ... sealed with adhesive and incubated for 10 min at 65°C and overnight at 37°C on a FISH-hybridizer (Dako; Agilent Technologies, Santa Clara, CA). The slides were then immersed in a series of baths with SSC buffer at different concentrations of 4× ...
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bioRxiv - Cancer Biology 2019Quote: ... 4°C hold) by Herculase II fusion enzyme (Agilent Technologies, #600679). After each step DNA was purified by Ampure XP beads (Beckmann Coulter Genomics #A63882) ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C after blocking with blocking solution (S3022, Dako) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... the photographs were taken by the C-Digit system (Agilent, USA).
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bioRxiv - Cell Biology 2022Quote: ... Splitless injection (injection temperature 270 °C) onto a DB-5MS (Agilent) was used ...
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bioRxiv - Immunology 2019Quote: ... and then overnight at 4°C in rabbit anti-CD3 (Dako) in 1% (v/v ...
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bioRxiv - Genetics 2020Quote: ... at 4°C followed by secondary antibody (Dako Cytomation, Glostrup, Denmark) for 30 min at 37°C ...