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Citations for Agilent :
851 - 883 of 883 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... around 50 ng of parent plasmid was taken and mutations were introduced by inverse PCR reaction using Pfu Ultra II polymerase (Agilent, cat no. 600674) enzyme using primer pairs having the respective mutations ...
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bioRxiv - Immunology 2024Quote: ... serum samples were prepared in a randomized order and measured in batches using dynamic molecular reaction monitoring on a liquid chromatography system (1260 Infinity II, Agilent Technologies, Waldbronn, Germany) coupled to a triple quadrupole mass spectrometer with electrospray ionization Agilent Jet Stream source ...
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bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: ... The samples were then further diluted in ultrapure water to a final 3% nitric acid concentration and analyzed by triple quadrupole inductively coupled plasma-mass spectrometry (Agilent 8800 ICP-QQQ). Copper concentrations were determined by using standard curves ...
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bioRxiv - Bioengineering 2023Quote: ... An aliquot of 1 mL was obtained and diluted with 2% nitric acid before running for elemental analysis using an inductively coupled plasma optical emission spectrometer (ICP-OES, Varian 720-ES, Agilent, Santa Clara, CA). For each run ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed on diluted lysis product (1:20 dilution) and 25 µl of PCR reaction mix according to the manufacturer instruction (Herculase II Fusion DNA Polymerase, Agilent Technologies, Les Ulis, France) using the following PCR primers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Point mutation of D5R-STOP from D5-TANGO was made by polymerase chain reaction (PCR) using the QuikChange II Site–Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The data were acquired via multiple reaction monitoring (MRM) using a 6495 Triple Quadrupole mass spectrometer coupled to an HPLC system (Agilent Technologies, Santa Clara, CA) through Agilent Mass Hunter Data Acquisition Software (ver ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was quenched with TFA and the peptides were isolated and desalted using reverse phase C18 and SCX cartridges (Agilent technologies, Santa Clara, CA). Tryptic peptide mixtures were evaporated with speed vacuum and dissolved in 2 % acetonitrile solution acidified with 1 % TFA at a final concentration of 2 μg/uL.
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bioRxiv - Biochemistry 2024Quote: ... Detection of acetylation was carried out by direct analysis of enzymatic reactions by LC-MS on an Agilent 1200 instrument coupled to an Agilent UHD 6530 Q-TOF mass spectrometer (Agilent Technologies Canada, Mississauga, ON) as described previously29.
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bioRxiv - Cell Biology 2020Quote: ... the DMT-On TMO oligonucleotide could be easily separated from failure products using a gradient of 50 mM Triethylammonium bicarbonate in acetonitrile (Agilent Zorbax C18 column, 2.0 mL flow rate). The DMT-On fractions were pooled ...
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bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were diluted with ultra-pure water to reduce the acid concentration below 3% and loaded into the ICP-MS-MS (triple quad Agilent 8800x ICP-MS-MS). The instrumental conditions were optimized to remove interferences by using a collision/reaction cell ...
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bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
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bioRxiv - Biochemistry 2022Quote: ... system coupled to an Agilent 6470 series QQQ mass spectrometer (MS/MS) was used to analyze fatty acids in each sample (Agilent Technologies, Santa Clara, CA). The methods are similar to what is described in the paper by Yang ...
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bioRxiv - Genomics 2024Quote: ... The measurement of the mineral nutrients in the acid digests was conducted by inductively coupled plasma optical emission spectroscopy (ICP-OES; Agilent 5110 Vertical Dual View). The measurements were verified by using certified standard reference material (SRM ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped with trifluoroacetic acid, and peptides were analyzed by RP-HPLC (Shimatzu, Kyoto, Japan) on an AdvanceBio Peptide Plus C18 column (Agilent Technologies, Santa Clara, CA). The samples (100 µL ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph, Agilent Technologies, Les Ulis, France) as previously described (25).
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bioRxiv - Molecular Biology 2021Quote: All PCR assays were carried out in 50 μl final volume in a reaction mixture containing 1X of Cloned Pfu DNA polymerase Taq (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative polymerase chain reaction (qPCR) reaction was carried out using the Brilliant III Ultra-Fast QPCR Master Mix in the Stratagene Mx3000P system (Agilent Technologies, Santa Clara, CA, USA). The thermal cycling protocol was ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-microliter reactions were prepared with 1×Brilliant III Ultra-Fast SYBR® Green Low ROX qPCR Master Mix (Agilent Technologies, Santa Clara, CA, USA), 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc. ...
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bioRxiv - Immunology 2024Quote: ... with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the manufacturer’s recommendations but using half-volume reactions. Qubit™ dsDNA HS (ThermoFischer Scientific, Cat no. Q32851) and Agilent 4200 TapeStation HS DNA1000 assays (Agilent, Cat no. 5067-5584) were used to measure quality and quantity of the generated libraries ...
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bioRxiv - Microbiology 2024Quote: ... Two amino acid substitutions (S299A and T309A) were carried out using specific primers (Table S1) and Pfu Turbo Polymerase (Agilent Technologies, Santa Clara, CA, USA). Then amplicons were further treated with DpnI (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... 2cm x 100µM) for 5min in solvent A (0.1% formic acid in water) before being separated on an analytical column (Agilent Poroshell, EC-C18, 2.7µM, 50cm x 75µM). Solvent B consisted of 0.1% formic acid in 80% acetonitrile ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... with solvent A (0.1% formic acid in water) at 30 µl/min flowrate and chromatographically separated over the analytical column (Poroshell 120 EC C18, Agilent Technologies, 50 μm x 75 cm, 2.7 μm) using 180 min gradient at 300 nL/min flow rate ...