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Citations for Agilent :
551 - 600 of 883 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed using the Agilent MXPro Real-Time PCR detection system (Agilent Technologies, Stratagene, USA). A total volume of 20µl contained10μl Brilliant III SYBR Green ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed using the Agilent MXPro Real-Time PCR detection system (Agilent Technologies, Stratagene, USA). A total volume of 20µl contained10μl Brilliant III SYBR Green ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Genetics 2021Quote: ... Real-time PCR reactions were performed with the Brilliant II SYBR® Green QPCR Master Mix (Agilent, 600828). The relative quantification in gene expression was carried out using the 2- ΔΔCt method (51) ...
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bioRxiv - Genomics 2021Quote: ... the PCR was performed in triplicate per ligation reaction using the Herculase II PCR reagents (Agilent Technologies, 600677). The parallel library preparations and PCR reactions were subsequently pooled for each reaction.
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bioRxiv - Systems Biology 2023Quote: ... The wPCR reaction was carried out on a AriaMx Real-Time PCR System (Agilent, Santa Clara, CA, USA) with 40 cycles of 15 s at 95 °C and 30 s at 60 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was performed in 20 μl SYBR Green reaction mixture using a Real-time PCR system (Agilent AriaMx Real-time PCR System ...
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bioRxiv - Immunology 2022Quote: ... Dynamics multiple reaction monitoring MS/MS was executed using a 6460 series triple quadrupole tandem mass spectrometer (Agilent) with electrospray ionization in negative mode ...
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bioRxiv - Plant Biology 2022Quote: ... was used for a qRT-PCR reaction carried out in a Stratagene Mx3005P qPCR machine (Agilent Technologies, USA). Three independent RNA isolations along with three technical replicates were used for mRNA quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... All site-directed mutagenesis (SDM) reactions were carried out using the Quick-Change Lightning SDM Kit (Agilent, USA), the primers listed in Supplementary Table S1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The qPCR reaction mixture consisted of 1X Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent), 0.002X reference dye (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... 10-µl reactions were prepared with the Brilliant III Ultra-Fast SYBR Green qRT-PCR Master Mix (Agilent) in technical duplicates for each sample ...
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bioRxiv - Microbiology 2022Quote: ... While the first polymerase chain reaction (PCR) was performed on a SureCycler 8800 (Agilent Technologies, Santa Clara, USA) using the following temperature profile ...
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bioRxiv - Plant Biology 2022Quote: ... was used for a qRT-PCR reaction carried out in a Stratagene Mx3005P qPCR machine (Agilent Technologies, USA). Five independent RNA isolations and three technical replicates were used for mRNA quantification ...
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bioRxiv - Genetics 2024Quote: ... PCR-2 reactions amplified vector fragments from MSp509_PKR-WT plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kb ...
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bioRxiv - Cancer Biology 2024Quote: ... and several immunohistochemistry reactions were performed on automated immunostaining platforms (Autostainer Link 48, Dako; Ventana Discovery XT, Roche).
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bioRxiv - Biochemistry 2024Quote: ... Reactions were initiated by addition of protein and monitored using a Cary Eclipse fluorometer (Agilent Technologies, Mississauga, Canada). Cleavage of Z-VLR-AMC was monitored using 380 nm and 450 nm excitation and emission wavelengths ...
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bioRxiv - Microbiology 2021Quote: ... Each lipid spot was extracted for quantification of fatty acids by gas chromatography-mass spectrometry (Agilent 5977A-7890B) after methanolysis ...
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bioRxiv - Bioengineering 2021Quote: ... Mutagenesis of individual amino acid residues in KRaION1 was done using the QuikChange Site-Directed Mutagenesis Kit (Agilent). Forward and reverse primers used to generate each mutant are provided in Supplementary Table 6 ...
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bioRxiv - Microbiology 2020Quote: ... and Sialic acids from the purified S-Ectodomain was performed by using a Deglycosylation kit (Agilent, CA, USA) as per the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Mobile phase B consisted of acetonitrile:water 9:1 with 10 mM ammonium acetate + 5 μM medronic acid (Agilent), pH 9 ...
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bioRxiv - Cell Biology 2020Quote: ... ShhHA and C25SShhHA (HA inserted between amino acids 197 and 198) were generated by site-directed mutagenesis (Stratagene). Unlipidated C25SShhN cDNA and non-palmitoylated C25AShh cDNA (amino acids 1-438 ...
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bioRxiv - Immunology 2021Quote: Short chain fatty acid concentrations were determined using gas chromatography (3800 Varian GC, Agilent Technologies, Santa Clara, CA). For each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Amino acid substitutions in the C-terminal (126-250) truncation were created via QuikChange site-directed mutagenesis (Agilent). pDEST-mCherry-LacR-RIF1 (1-567 ...
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bioRxiv - Neuroscience 2023Quote: ... Single amino acid substitutions of GLIC were engineered using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) and oligonucleotide primers (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 μL of acetic acid was added to the sample before desalting with a C18 tip (Agilent A57203). The C18 tip was washed three times with 200 μL of methanol ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatographic separations of free amino acids were carried out using a HILIC-Z column (2.1×150 mm, particle size 2.7 µm, Agilent) employing a linear binary gradient of (A ...
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bioRxiv - Plant Biology 2024Quote: ... Oligosaccharides were reductively aminated with 2-aminobenzoic acid (2-AA) and cleaned using a GlycoClean S cartridge (ProZyme) as previously described (Tryfona & Stephens ...
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bioRxiv - Immunology 2024Quote: ... Eluted tryptic peptides were acidified with 1% v/v formic acid and desalted with C18 Omix tips (Agilent).
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bioRxiv - Plant Biology 2024Quote: Methyl ester derivatives of total fatty acids (FAMEs) were analysed by Gas Chromatography (GC) (Agilent 7890A, Agilent Technologies) using an Agilent J&W 122-2332 column (30 m × 250 µm × 0.25 µm ...
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bioRxiv - Plant Biology 2024Quote: Methyl ester derivatives of total fatty acids (FAMEs) were analysed by Gas Chromatography (GC) (Agilent 7890A, Agilent Technologies) using an Agilent J&W 122-2332 column (30 m × 250 µm × 0.25 µm ...
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bioRxiv - Biochemistry 2024Quote: ... Automated dilution was performed using 5 μL of solvent A (99.9% water + 0.1% formic acid) with the Bravo liquid handler (Agilent). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... samples were diluted to 1 mg/mL in 0.2% formic acid and injected via HPLC (Agilent 1100 HPLC) and eluted in a mobile phase of formic acid/methanol ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were then gel extracted and cloned into a vector using the Strataclone PCR Cloning kit (Agilent, Santa Clara, CA) and Sanger sequenced using the BigDye Terminator Cycle sequencing kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleavage products were separated on the Fragment Analyzer™ using the CRISPR Mutation Discovery Kit (Agilent Technologies, Santa Clara, CA, USA). Editing and HDR frequencies were calculated using the following formula ...
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bioRxiv - Immunology 2022Quote: ... Human B Cell Kit (10X Genomics) and the quality and quantitation of the products were monitored using the High Sensitivity DNA Kit (Agilent Technologies). Libraries were sequenced on an Illumina sequencer with Illumina adaptor primers found in the Chromium i7 Multiplex Kit.
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bioRxiv - Neuroscience 2024Quote: Mouse hind paws were fixed in Zamboni’s fixative for 24-48 hours at 4°C and processed as previously described.44 50 μm floating tissue sections were stained with the primary antibody rabbit anti-protein-gene-product 9.5 (PGP 9.5) (Dako, Z5116, 1:500) overnight and the secondary biotinylated goat anti-rabbit IgG antibody (Vector Laboratories ...
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bioRxiv - Immunology 2024Quote: ... The quality of whole transcriptome amplification (WTA) product was assessed using the Agilent High Sensitivity D5000 Screen Tape System (Agilent Genomics) with an expected peak >800 bp tailing off to beyond 3000 bp and a small/nonexistent primer peak ...
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bioRxiv - Microbiology 2022Quote: ... The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA 7500 kit (Agilent Technologies).
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
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bioRxiv - Genetics 2022Quote: ... and product concentration quantified with Agilent 4150 TapeStation using the Agilent Genomic DNA ScreenTape Assay (Agilent Technologies, Santa Clara, CA, USA). Samples of the respective PCR products were pooled in equimolar concentration and libraries were prepared using the Genomic DNA by Ligation (SQK-LSK109 ...
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bioRxiv - Microbiology 2022Quote: ... The Agilent High Sensitivity DNA Kit for naturally contaminated shellfish was used to visualise second-round PCR products for Bioanalyzer 2100 (Agilent Technologies).
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bioRxiv - Synthetic Biology 2022Quote: ... The freeze-dried products were identified via analytical HPLC-MS on an Agilent 1260 Infinity II Series HPLC-system (Agilent Technologies) using column 2 (Supplementary Table 13) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified with Agencourt AMPure XP beads and sample profile was analysed with the High Sensitivity D5000 kit (Agilent Technologies) on a TapeStation (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... The product 6’-Cl-2’3’-cG(A)MP was purified using HPLC on a PFP Poroshell 120 column (Agilent Technologies, Inc.) with Acetonitrile/0.1% Formic Acid as the mobile phase ...
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bioRxiv - Biophysics 2024Quote: ... The final product was purified by preparative high-performance liquid chromatography (HPLC) on a Zorbax 300SBC3 reverse-phase column (Agilent Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... and several immunohistochemistry reactions were performed in an automated immunostaining platform (Autostainer Link 48, Dako; Ventana Discovery XT, Roche). Antigen retrieval was first performed with the appropriate pH buffer ...
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bioRxiv - Biochemistry 2020Quote: The DNA template was amplified by the polymerase chain reaction (PCR) using Pfu high-fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of gDNA (∼27 ng ...
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bioRxiv - Cell Biology 2022Quote: ... Triplicate reactions were set up in Semi-skirted 96-Well PCR Plates (0.2 ml) with optical strip caps (Agilent). The PCR reactions were carried out in an AriaMx Real-time PCR System (Agilent) ...