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Citations for Agilent :
501 - 550 of 883 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and β-amyloid analysis (pretreatment with formic acid, antibody 4G8, DAKO, M087201-2, 1:100, 30 minutes). Tau pathology was assessed using Braak criteria (Braak et al ...
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bioRxiv - Microbiology 2023Quote: ... Amino acid analysis was performed by high-pressure liquid chromatography (HPLC) (Agilent 1100; Agilent Technologies, Massy, France) with a guard cartridge and a reverse phase C18 column (Zorbax Eclipse-AAA 3.5 μm ...
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bioRxiv - Cell Biology 2024Quote: The Peptide nucleic acid (PNA) probe hybridisation of chromosome spreads followed the manufacturers guidelines (DAKO Agilent & PNAbio). Chromosome spreads were fixed in 3.7% PFA and washed using a gradient ice-cold ethanol wash series 70% ...
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bioRxiv - Cell Biology 2024Quote: The Peptide nucleic acid (PNA) probe hybridisation of chromosome spreads followed the manufacturers guidelines (DAKO Agilent & PNAbio). Chromosome spreads were fixed in 3.7% PFA and washed using a gradient ice-cold ethanol wash series 70% ...
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bioRxiv - Plant Biology 2024Quote: ... Amino acid contents were measured using a triple quadrupole LC-MS/MS system (Agilent 6420, CA, USA) with a Discovery HS-F5 column (2.1 × 250 mm ...
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bioRxiv - Biochemistry 2024Quote: ... Bond Elut PBA (phenylboronic acid) cartridge and 96-well plate were purchased from Agilent (Santa Clara, USA). 1 µM of q and Q was spiked in human plasma and extracted with SPE cartridges ...
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bioRxiv - Microbiology 2024Quote: ... and dispensed 30% acetic acid using the BioTek EL406 microplate washer/dispenser (Agilent, Santa Clara, CA, USA). The absorbance of CV-stained biofilms was recorded at 590 nm ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... Size distribution and concentration of the final products were then evaluated using the Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies) and loaded onto an S1 flow cell on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... product size distribution and quantity were assessed on a Bioanalyzer using a High Sensitivity DNA Kit (Agilent Technologies 5067-4628). A total of 140 pg of the amplified cDNA was fragmented using Nextera XT DNA sample preparation kit (Illumina FC-131-1096 ...
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bioRxiv - Immunology 2021Quote: ... Product was amplified and barcoded with adaptor specific primers and the quality of the resulting libraries were determined by Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... Bisulfite-converted DNA was used for PCR amplification of the DMRs and products were visualized on a 3% Tris-Acetate-EDTA agarose gel and quantified by TapeStation (Agilent).
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bioRxiv - Cell Biology 2021Quote: ... The dDia2-CA mutant was created by first PCR-amplifying the forH gene (using forH4L and forH8 oligos) from GFP-dDia2 (Schirenbeck et al., 2005) then TA cloning the product using StrataClone (Agilent) to generate dDia2-SC ...
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bioRxiv - Microbiology 2022Quote: ... The concentration and size distribution of generated double-stranded cDNA products were estimated by the TapeStation 4200 system (Agilent Technologies). Sequencing libraries were prepared from 50ng of DNA according to the manufacturer’s preparation guide #112219 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Quality and quantity of PCR products were determined by using an Agilent 4150 TapeStation with High Sensitivity D5000 ScreenTape (Agilent). Final libraries were indexed using one of the 16 indexes for Illumina sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... The quality and concentration of the PCR products was determined using a 2200 TapeStation and D1000 screen tapes (Agilent Technologies). To generate the NGS libraries ...
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bioRxiv - Systems Biology 2020Quote: ... The size of the digested product (~105bp) and the undigested sample (~150bp) was verified by running them on a TapeStation (Agilent) screentape (Supplemental Figure 1a) ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Product identity and purity were confirmed by Time-Of-Flight Liquid Chromatography-coupled Mass Spectroscopy (TOF LC-MS, Agilent 6230). Lyophilised samples were resuspended in 32 μl water immediately prior to use.
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bioRxiv - Genetics 2020Quote: ... The final purified product was then quantified and qualified using the TapeStation DNA screen tape (Agilent, Santa Clara, CA, USA). For exome capture ...
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bioRxiv - Genetics 2020Quote: ... The previously reported RALY intronic duplication 23 was genotyped by size differentiation of PCR products on a Fragment Analyzer (Agilent). Five PCR-assays (ventral promoter assays 1 ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape ...
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bioRxiv - Biochemistry 2020Quote: ... Mass signal intensities corresponding to the product (TAFTIPSI) and internal standard (Ac-YTAFTIPSI) were measured on a Rapidfire autosampler (Agilent) equipped with a C18 solid phase cartridge ...
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bioRxiv - Immunology 2020Quote: ... Amplified products were solid phase reversible immobilization (SPRI) bead-purified and evaluated by Fragment Analyzer (Agilent, Santa Clara, CA, USA). Twenty-five percent of the cDNA volume was subjected to fragmentation and double-sided SPRIselect (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... LC-MS analysis to determine the heterologous expression product of hpdA was performed on a liquid chromatograph (Agilent 1200, USA) equipped with an Eclipse XDB-C18 reverse-phase column (5 μm ...
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bioRxiv - Genomics 2021Quote: ... the initial concentrations of purified SARS-CoV-2 and the universal human reference RNA (UHRR, Agilent Technologies, product number 740000) were determined by ddPCR as described above ...
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Legionella relative abundance in shower hose biofilms is associated with specific microbiome membersbioRxiv - Microbiology 2023Quote: ... the products were quantified and checked for correct length (bp) with the High Sensitivity D1000 ScreenTape system (Agilent 2200 TapeStation). Based on their concentration ...
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bioRxiv - Immunology 2023Quote: ... PCR products of enhancers were amplified in a mix of PfuUltra DNA Polymerase (600385-51, Agilent Technologies, Santa Clara, CA) and Taq DNA polymerase (M7123 ...
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bioRxiv - Cancer Biology 2023Quote: ... The purified capture products was then amplified using the SureSelect Post-Capture Indexing forward and Index PCR reverse primers (Agilent) for 12 cycles ...
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bioRxiv - Immunology 2023Quote: ... The quality control of the final library products was performed using the Qubit Fluorometer and the Agilent 2100 bioanalyzer with a High Sensitivity Kit (Agilent). Library products were pooled to a final concentration of 4 nM ...
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bioRxiv - Plant Biology 2023Quote: ... Hexane extract from plant and in vitro biochemical assay products were initially analysed in GC (Agilent Technologies, 7890B and 5977A) using HP-5 MS column (0.25 mm diameter ...
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bioRxiv - Microbiology 2024Quote: ... the products were quantified and checked for correct length (bp) with the High Sensitivity D1000 ScreenTape system (Agilent 2200 TapeStation). Sample concentration was adjusted ...
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...
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bioRxiv - Genetics 2023Quote: ... 128-nt oligos with pgRNA-coding sequences were synthesized with Agilent oligo library synthesis service (Agilent OLS, product No. G7261A) and then cloned into pCG-EGFP backbone using Gibson assembly ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ng of extracted PCR products were analyzed by an Agilent High Sensitivity DNA kit on an Agilent 2100 Bioanalyzer (Agilent), and the remainder was cloned by using PCR Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and the gRNA synthesis was checked for correct product size with an RNA Nano Chip Assay on a 2100 Bioanalyzer device (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... The final product was assessed for size distribution and concentration using the BioAnalyzer High Sensitivity DNA Kit (Agilent, 5067-4626). Pooled libraries were diluted to 2 nM in EB buffer (Qiagen ...
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bioRxiv - Biophysics 2021Quote: The error-prone PCR reaction for libraries “SH3_EP” was done using the GeneMorph II Mutagenesis Kit (Agilent Technologies) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was then stopped by adding 2μl of 0.25M EDTA and analyzed with the Fragment Analyzer 5200 (Agilent) capillary electrophoresis instrument and the CRISPR Discovery Gel Kit (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... Two DNA fragments were generated by polymerase chain reaction (PCR) using Herculase II Fusion DNA polymerase (Agilent Technologies), primers P11 + P12 and P13 + P14 and plasmids pUCAP-pGEL3::spmCherry-pATrpC-BAR (Samalova et al. ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions contained 10 μL of 2×Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent), 2 μL of each 2 μM primers (Table S5) ...
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bioRxiv - Genetics 2020Quote: ... Captured libraries were amplified in 3×100 μl reactions containing 1 unit Herculase II Fusion DNA polymerase (Agilent), 1x Herculase II reaction buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... and the reaction was performed on an Mx3000P real-time PCR machine (Agilent Stratagene, Santa Clara, CA, USA). The levels of target gene mRNA transcripts relative to control β-actin or gapdh were determined using the 2−ΔΔCt method30 ...
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bioRxiv - Developmental Biology 2020Quote: ... and the reaction was performed on an Mx3000P real-time PCR machine (Agilent Stratagene, Santa Clara, CA, USA). The levels of target gene mRNA transcripts relative to control β-actin or gapdh were determined using the 2−ΔΔCt method30 ...
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bioRxiv - Cell Biology 2021Quote: ... All IHC staining was completed using chromogenic enzyme substrate reactions with DAB (Agilent Technologies, Santa Clara, CA, USA). Secondary antibody incubation and chromogenic reactions are then performed using an automated INTELLIPATH FLX system (Biocare Medical ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were performed in duplicate using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) on a C1000 Touch™ thermo Cycler using the CFX96™ Real-Time System (BioRAD) ...
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bioRxiv - Physiology 2020Quote: ... All qPCR reactions used SYBR® Brilliant III Ultra-Fast QBRR Green Prime Mix Low ROX (Agilent Technologies). Beta Actin was used as an endogenous control ...