Labshake search
Citations for Agilent :
651 - 700 of 883 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed in PBS 5 x 5 mins, then mounted with a cleaned glass coverslip (#1.5, 24 mm x 60 mm) in mounting media (Dako; Pathology Products, S3023).
-
bioRxiv - Microbiology 2023Quote: ... DNA concentration of each PCR product was quantified using Qubit® 4.0 Green double-stranded DNA assay and assessed for quality with a bioanalyzer (Agilent 2100, USA). Libraries ...
-
bioRxiv - Genetics 2020Quote: ... Each reaction were carried out in technical triplicates with Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent) on the LightCycler 480 Instrument (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... ∼100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2020Quote: ... to avoid cross-reaction during the incubation of the following cocktail of primary antibodies: mouse monoclonal anti-pan-cytokeratin (clones AE1/AE3, DAKO) to detect the epithelial compartment ...
-
Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... qRT–PCR reactions were performed in duplicate using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) on a C1000 Touch™ thermos Cycler CFX96™ Real-Time System (BioRAD ...
-
bioRxiv - Bioengineering 2021Quote: ... All PCR reactions for cloning and amplification of sequencing templates were performed using Herculase II Fusion DNA Polymerase (Agilent Technologies), and using GoTaq (Promega ...
-
bioRxiv - Neuroscience 2021Quote: ... were used to amplify mbp transcripts within a qPCR reaction (Brilliant III Ultra-fast SYBR Green qPCR Master Mix, Agilent). Transcript levels were detected using Roche Light Cycler 96 (Roche Life Science ...
-
bioRxiv - Biochemistry 2020Quote: The supernatants of the quenched in vitro OMT reactions and fermentation samples were analyzed by reversed-phase HPLC (instrument: Agilent 1100 ...
-
bioRxiv - Systems Biology 2020Quote: ... 3.5 μl/well was then used as template for 2nd PCR in a 50 μl reaction together with 0.5 μl Herculase II fusion DNA polymerase (Agilent Technologies), 10 μl 5* Herculase II reaction buffer ...
-
bioRxiv - Biophysics 2022Quote: All of mutations in this study were made by using overlap-extension polymerase chain reaction (PCR) with Pfu polymerase (Stratagene) on the template of the mbr5 splice variant of mslo1 (Uniprot ID ...
-
Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... The separation after injection of the complete iodination reaction was performed utilizing a Zorbax 300SB-C18 column (Agilent, Waldbronn, Germany) with elution of bound peptide by a water-acetonitrile gradient from 10 to 60% containing 0.1% TFA within a runtime of 60 minutes.
-
bioRxiv - Microbiology 2020Quote: ... and 1 μl was used for a 10 μl qPCR reaction with 5 μl THUNDERBIRD SYBR Green mix (Toyobo) on an Mx3000P qPCR System (Agilent) using the following program ...
-
bioRxiv - Molecular Biology 2020Quote: ... Converted libraries were enriched by 10 cycles of PCR with the following reaction composition: 1 μl Pfu TurboCx Hotstart DNA polymerase (Stratagene), 5 μl PfuTurbo Cx reaction buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... in 384-well plates on 5 μL scale reactions using 2 μL diluted cDNAs and Brilliant III Ultra-fast SYBR Green qPCR Master Mix (Agilent) with primers listed in Table S7 at a final concentration of 0.4 μM ...
-
bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
-
bioRxiv - Microbiology 2022Quote: Phenylhydrazine and aniline in the culture and enzymatic reaction solutions were detected by high-performance liquid chromatography (HPLC, Agilent 1260). HPLC analysis utilized an Agilent Eclipse XDB-C18 (4.6 mm ×250 mm ...
-
bioRxiv - Genetics 2022Quote: ... d/ the output was changed for qPCR analyses: polymerase reactions were carried out in triplicate in 96-well format using a Mx3005P qPCR machine (Agilent) equipped with FAM filters (λex = 492 nm ...
-
bioRxiv - Microbiology 2021Quote: ... Specific antigen-antibody reactions were visualized by means of 3,3’-diaminobenzidine tetrahydrochloride staining using the Dako Envision system (Dako Cytomation).
-
bioRxiv - Biochemistry 2021Quote: ... AccuScript high-fidelity first-strand cDNA synthesis kit for reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was procured from Agilent.
-
bioRxiv - Cell Biology 2020Quote: ... 6 PCR reactions including two biological replicates and 3 technical replicates per gene were performed using Brilliant SYBR master mix (Agilent), running cycles of 10 minutes at 95°C ...
-
bioRxiv - Microbiology 2021Quote: ... three independent PCR reactions were carried out in a final volume of 50 µl with Herculase II fusion DNA polymerase (Agilent), then pooled and confirmed on gel electrophoresis for both fungi and bacteria ...
-
bioRxiv - Genomics 2021Quote: ... in a final reaction volume of 40 μl following Carøe et al.45 and amplified using PfuTurbo Cx HotStart DNA Polymerase (Agilent Technologies) and Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc ...
-
bioRxiv - Immunology 2023Quote: ... The reaction was stopped by adding STOP solution and the absorbance at 450 nm using a BioTeck Gen5 plate reader (Agilent).
-
bioRxiv - Microbiology 2022Quote: ... Quantitative PCR was performed in a 25 μL reaction volume consisting of 12.5 μL Brilliant II SYBR Green Master Mix (Agilent Technologies), 1.0 μL of 10 μM forward DSR-1F (5’-ACS CAC TGG AAG CAC GGC GG −3’ ...
-
bioRxiv - Cancer Biology 2023Quote: ... and all RT-qPCR reactions were performed on an AriaMx Real-Time PCR System instrument (Agilent Technologies Santa Clara, California). Thermal cycling conditions were as follows:
-
bioRxiv - Microbiology 2023Quote: ... Specific antigen-antibody reactions were visualized by means of 3,3’-diaminobenzidine tetrahydrochloride staining using the Dako Envision system (Dako Cytomation). Histopathological severity score of pneumonia was determined based on the percentage of alveolar inflammation in a given area of a pulmonary section collected from each animal in each group using the following scoring system ...
-
bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2024Quote: ... four sequential site-directed mutagenesis reactions were performed following the QuikChange II XL Site-Directed Mutagenesis Kit protocol (Agilent, 200521). The following primers were generated using Agilent’s QuikChange Primer Design tool ...
-
bioRxiv - Molecular Biology 2022Quote: 1 µL of ChIP eluate was used for quantitative real-time PCR (qPCR) in 10 μl reactions with Brillant III Ultra Fast SYBR-Green Mix (Agilent) using a Stratagene MX3005p system ...
-
bioRxiv - Developmental Biology 2024Quote: ... The resulting cDNA was amplified with an overnight in vitro transcription reaction and the quality of the RNA was evaluated using TapeStation (Agilent). From this amplified RNA ...
-
bioRxiv - Molecular Biology 2024Quote: ... qRT-PCR reactions were performed in duplicate using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) on a C1000 Touch™ thermos Cycler using the CFX96™ Real-Time System (BioRAD ...
-
bioRxiv - Genomics 2024Quote: ... The components were assembled using a golden gate reaction (with BsaI and T4 ligase) and transformed into XL10 gold ultracompetent bacteria (Agilent). Lentiviral engineered pegRNA plasmids were cloned the same way but using a lentiviral epegRNA acceptor construct as starting point (Supplementary Table 1 ...
-
bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis was carried out utilizing a multiple reaction monitoring method for analyte detection on an Agilent LC-1290 Infinity II (Agilent Technologies ...
-
bioRxiv - Immunology 2022Quote: ... 60ng of DNA was used for PCR mix reaction prepared according to the manufacturer protocol (Herculase II Fusion DNA Polymerase, Agilent). The following Cre primers were used to carry out the PCR amplification ...
-
bioRxiv - Biochemistry 2022Quote: ... and the progress of the reaction was continuously monitored by tracking the decrease in absorbance at 340 nm using a Cary UV 60 spectrophotometer (Agilent). The initial rate was determined by calculating the slope of product release in the linear portion of the reaction ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were subjected to positive electrospray ionization (ESI) and detected via multiple reaction monitoring (MRM) using a LC-MS/MS system (Agilent Technologies 6460 QQQ/MassHunter ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR mixes were made up as follows in a total volume of 50 µL: 1X Pfu reaction buffer (Agilent, #600250), 0.2 mM dNTP (NEB® ...
-
Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent). Sanger sequencing (Microsynth ...
-
bioRxiv - Physiology 2024Quote: ... qPCR reactions were performed in triplicates using Brilliant III Ultra-fast SYBR Green qPCR Master Mix with low ROX (Agilent) according to the manufacturer’s instructions with the following cycling conditions ...
-
bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
-
bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
-
bioRxiv - Cell Biology 2020Quote: Samples from all biological replicates were first sent to the Stanford University Protein and Nucleic Acid Facility (Stanford, CA) for quantification and quality analysis using a 2100 Bioanalyzer (Agilent). Samples were then sent to Novogene Corporation Inc ...
-
bioRxiv - Microbiology 2021Quote: ... Fatty acid methyl esters were identified by their mass spectrum and retention time and quantified by Mass Hunter Quantification Software (Agilent) and the calibration curve generated with fatty acid methyl esters standards mix (Sigma CRM47885) ...
-
bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
-
bioRxiv - Immunology 2020Quote: ... The codon encoding residue 241 of Copa was mutated from glutamic acid to lysine via Quikchange Lightning site directed mutagenesis (Agilent). Following NotI and PacI digestion ...
-
bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
-
bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
-
bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...
-
bioRxiv - Biophysics 2020Quote: ... Chromatographic separation of OPA-derivatized amino acids was performed using an Agilent ZORBAX Eclipse AAA column (4.6mm x 150 mm x 3.5 μm; Agilent #963400-902) coupled with a ZORBAX Eclipse AAA Analytical Guard Column (4.6 mm x 12.5 mm x 5 μm ...