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Citations for Agilent :
651 - 700 of 1553 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biophysics 2020Quote: ... a point mutation was introduced into the WT plasmid using the QuikChange site-directed mutagenesis method (Agilent). HEK-293 cells were transiently transfected with NaChBac WT or T220A cDNA using Lipofectamine™ 2000 transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The required vector was transformed into Escherichia coli BL21 (DE3) cells containing the RIL Camr plasmid (Stratagene) for expression analysis.
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bioRxiv - Microbiology 2022Quote: ... and were used to PCR amplify around the pMMB67EH plasmid using PfuUltra II high-fidelity polymerase (Agilent). PCR products were purified (QIAGEN ...
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bioRxiv - Biophysics 2019Quote: ... Histone expression plasmids were from Narlikar lab and BL21(DE3)pLysS competent E.coli cells were from Agilent Technology (USA) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... point mutations were introduced in the pIRES2-mCherry-YFPCFTR plasmid using site-directed mutagenesis (Quikchange protocol, Stratagene).
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bioRxiv - Cell Biology 2019Quote: Generation of pGDB-SDEL plasmid was done using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) on the pGDB plasmid with primers P1 and P2 per the manufacturer’s protocol36.
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bioRxiv - Microbiology 2021Quote: ... berghei gDNA using primers 9483/9484 and 9485/9486 respectively and introduced sequentially into pBSKS+ plasmid (Stratagene) at XhoI/BamHI and EcoRI/SacII restriction sites respectively ...
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bioRxiv - Biophysics 2022Quote: ... Site-directed mutagenesis were introduced into plasmids using QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) and confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 μg of each plasmid was used to transfect ring stage parasites ...
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bioRxiv - Neuroscience 2022Quote: ... The pT7-7 plasmid was also modified using site directed mutagenesis (#200523, QuikChange II, Agilent, Waldbronn, Germany) to incorporate a cysteine residue at position 141 to permit dye-labelling of the aSyn protein ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Plasmid and cDNA libraries were pooled and quality was evaluated using capillary gel electrophoresis (Agilent Bioanalyzer 2100). Sequencing was performed on an Illumina HiSeq 1500 instrument using a single-index ...
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bioRxiv - Microbiology 2023Quote: ... Site-directed mutagenesis on the pmPol I-LASV Sag plasmid was performed using QuikChange II kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The Nir1-LNS2 plasmid was transformed into competent BL21 (DE3) RIPL cells (Agilent Technologies, Cat. No. 230280) for protein overexpression ...
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bioRxiv - Developmental Biology 2023Quote: ... and the fragments were incorporated into a pBluescript II SK (−) vector plasmid (Agilent Technologies Japan, Hachioji, Japan). Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... I5S mutant (mt) RIIα-mTb-V5 plasmid was generated by site directed mutagenesis (QuikChange II XL, Agilent) based on the wild type (WT ...
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bioRxiv - Microbiology 2023Quote: Plasmids were isolated from 250 mL cultures of Escherichia coli (XL10-Gold Ultracompetent Cells, Agilent Cat. 200314) and 60 µg of pDC2-MORC-HA or linearized pKD-MORC-HA-TetR-DOZI ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were introduced into plasmids of interest using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies; 200516) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... mutations were incorporated into PLEKHA4 and PLEKHA5 plasmids using the QuikChange XL Site-directed mutagenesis kit (Agilent). A nuclear export signal with the amino acid sequence LQLPPLERLTLD was inserted either between PLEKHA5 and GFP or downstream of GFP into the PLEKHA5H-BP-PH-EGFP plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... The mutation A243V was introduced into the GluN2A plasmid using the QuickChange II XL kit from Agilent. All portions of the resulting construct that were subject to PCR were confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... or mouse monoclonal anti-CD68 (Cat. No. MO814, Dako, Santa Clara, CA) for 45 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hr with polyclonal rabbit-anti-mouse Ig antibody (Dako) in PBS-T + 0.1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... (12) mouse anti-PCNA was used at 1:1000 (M0879; Dako Immunochemicals). This antibody was raised to a synthetic amino acid sequence (LVFEAPNQEK ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used in IHC assays include: mouse-anti-human CD3 (Dako, F7.2.38), rabbit-anti-human Granzyme B (eBiosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human CD20 (Dako, clone L26, 1:1000, pH 6 retrieval), mouse anti-human FOXP3 (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies: horseradish peroxidase (HRP)-conjugated polyclonal rabbit-anti-mouse (Dako, P0260), HRP-conjugated polyclonal swine-anti-rabbit (Dako ...
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bioRxiv - Cancer Biology 2019Quote: ... Horseradish peroxidase-conjugated antibody anti-mouse IgG and anti-rabbit IgG (Dako) were used to detect immunoreactive bands and binding was revealed using enhanced chemiluminescence (Pierce) ...
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bioRxiv - Cancer Biology 2020Quote: ... cRNA were hybridized to SurePrint G3 Mouse Gene Expression 8×60K (Agilent) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... and the respective secondary antibody goat anti-mouse-HRP (1:3000, DAKO) were used ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-Pan-Cytokeratin (1:100, M351529-2, Dako Agilent Pathology Solutions), rabbit anti-CD3 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-Pan-Cytokeratin (1:100, M351529-2, Dako Agilent Pathology Solutions), rabbit anti-CD3 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A., Spain) was used for the qualitative identification of antigens by light microscopy ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... and the secondary antibody was HRP-conjugated rabbit anti-mouse (DaKo, P0161) diluted 1:5000.
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bioRxiv - Cancer Biology 2020Quote: ... Sections were stained with the following antibodies: mouse monoclonal anti-PTEN (Dako), rabbit polyclonal anti-p62 (Enzo Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-SMA (1:1000; M0851; Clone14A; Dako, Denmark AS, Denmark) was labeled with Alexa Fluor 647 tyramide solution ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were applied as follows: EnVision polymer reagent for mouse (Dako) at room temperature for 45 min or a biotin-labeled anti-rat IgG (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... An horseradish peroxidase (HRP)-coupled rabbit anti-mouse antibody (Dako, Glostrup, Denmark) in combination with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HRP conjugated secondary antibody goat anti-mouse (Dako) were diluted in 0.5X blocking buffer/PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies anti-mouse-HRP and anti-rabbit HRP were from Dako and membranes were developed using ECL prime and imaged in Bio-Rad imager using software from Bio-Rad.
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bioRxiv - Neuroscience 2021Quote: ... Gap43 antibodies were visualized with biotin-conjugated rabbit anti-mouse antibodies (Dako) followed by streptavidin-conjugated Cy3 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: ... Horseradish peroxidase-conjugated antibody anti-mouse IgG and anti-rabbit IgG (Dako) were used to detect immunoreactive bands and binding was revealed using enhanced chemiluminescence (Pierce) ...
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bioRxiv - Cancer Biology 2020Quote: ... and B lymphocytes (anti-CD20 mouse IgG2a, 1:150, clone L26, Dako). Tumor epithelial cells were detected using anti-pan-cytokeratin Alexa 488-conjugated mouse IgG1 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... using a CD68 clone KP1 mouse monoclonal antibody (Dako/Agilent cat #M0814) at a 1:100 dilution to identify macrophages ...
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bioRxiv - Microbiology 2020Quote: ... using a CD68 clone KP1 mouse monoclonal antibody (Dako/Agilent cat #M0814) at a 1:100 dilution to identify macrophages ...
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bioRxiv - Cancer Biology 2021Quote: ... The anti-CD8 antibody (1:5,000, C8/144B, mouse monoclonal antibody, Agilent) was detected using the Opal 520 fluorophore (1:150 ...
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bioRxiv - Neuroscience 2022Quote: ... DR (‘anti-MHC II’, mouse IgG1, Dako clone CR3/43, 1:150), anti-Calbindin (mouse IgG1 ...
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bioRxiv - Systems Biology 2022Quote: ... or horseradish peroxidase (HRP-conjugated polyclonal anti-mouse/anti-rabbit immunoglobulins; Dako) diluted 1:5,000 in 5% non-fat dry milk–TBST for 1 h at room temperature ...