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Citations for Agilent :
701 - 750 of 2353 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coli cells after was taken for RFP analysis using fluorescence spectrophotometer (Cary Eclipse, Agilent) with excitation wavelength of 550 nm ...
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bioRxiv - Biophysics 2023Quote: ... These histones were co-expressed in Escherichia coli strain BL21-codonplus-DE3-RIL (Agilent), and the histone octamer with the K120>C mutation in histone H2A was purified according to Ref33 ...
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bioRxiv - Biophysics 2023Quote: ... Aβ42 was expressed in the Escherichia coli BL21Gold (DE3) strain (Stratagene, La Jolla, CA) and purified by sonication and dissolving the inclusion bodies in 8 M urea ...
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bioRxiv - Biochemistry 2024Quote: The codon-optimized plasmid for hPLIN1 was expressed in Escherichia coli ArcticExpress (DE3) (Agilent) while codon optimized plasmids hPLIN2 and hPLIN3 were expressed in E ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression plasmids were transformed into Escherichia Coli strain BL21-Codonplus(DE3)-RIL (Stratagene). The expression and purification of each protein was performed as described previously.77 Briefly ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Microbiology 2019Quote: All mutated MERS-CoV S constructs were generated by site directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent). pDNA3.1+/MERS-CoV S EMC/2012 served as template for all the mutagenesis performed ...
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bioRxiv - Molecular Biology 2019Quote: ... The immobilized Hi-C libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with eight PCR amplification cycles ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were separated on a Hi-Plex Na column by high-performance liquid chromatography (HPLC) (Agilent Technologies) by applying a flow rate of 0.7 mL/min with water and methanol as mobile phase ...
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bioRxiv - Microbiology 2023Quote: Pharmaceuticals were analyzed using high-performance liquid chromatography (HPLC) with a Hi-Plex Na column (Agilent Technologies). The separation was achieved by applying a flow rate of 0.7 mL/min ...
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bioRxiv - Cell Biology 2021Quote: ... The E-box mutant was constructed with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using primers 5’-cgcgggttcccaccttgtggccagaagatcttctgggcagaactactcgtttggc-3’ and 5’-gccaaacgagtagttctgcccagaagatcttctggccacaaggtgggaacccgcg-3’ ...
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bioRxiv - Microbiology 2022Quote: ... RTCA E-plate VIEW 16 plates with embedded golden electrodes (#300600880, Agilent, Santa Clara, CA, USA) were used for the experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissues were stained using H&E or a primary antibody against Ki67 (GA626, Dako Omnis) and an HRP-labeled secondary antibody (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 x 104 tumor cells were plated per well in a 96-well E-plate (Agilent), and impedance is read for 16 hours during incubation ...
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bioRxiv - Plant Biology 2020Quote: ... a 6x Histidine tag was appended to the C-terminus of rbcS via the Quikchange protocol (Stratagene). Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen) ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... PARLSkd3 and variants were expressed with an N-terminal MBP-tag in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in lysis buffer (40 mM HEPES-KOH pH = 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3C library preparation and target enrichment using a custom-designed collection of 6979 biotinylated RNA “baits” targeting single MboI restriction fragments chr15:10283500-13195800 (mm9) (Suppl. Table S2; Agilent Technologies; as in Ref.3) were performed following the SureSelectXT Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Biophysics 2024Quote: In vitro NMR experiments were carried out at 25 °C on an 800 MHz Agilent spectrometer equipped with a cryogenic probe (Agilent Technologies). The buffer used in all NMR titration assay was 50 mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... to purify the library material and confirmed that the library DNA was in the size range of 200-800 bp using high sensitivity DNA Chip detection (Bioanalyzer 2100, Agilent Genomics), and sequenced the libraries on a NextSeq 500 instrument (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... All AtAtm3 constructs were overexpressed in Escherichia coli BL21-gold (DE3) cells (Agilent Technologies, CA) using ZYM-5052 autoinduction media as described previously (Fan et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Microbiology 2021Quote: All transformations for cloning or plasmid amplification were performed in Escherichia coli XL10 Gold (Agilent) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2022Quote: ... Aβ40/Aβ42 was expressed in the Escherichia coli BL21Gold (DE3) strain (Stratagene, La Jolla, CA) and purified by sonication and dissolving the inclusion bodies in 8 M urea ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Neuroscience 2022Quote: ... The insert was verified by sequencing and then transformed into BL21 Escherichia coli strain (Stratagene) for expression ...
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bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
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bioRxiv - Microbiology 2019Quote: ... GFAP (Dako #M076101-2 or #Z033429-2), OLIG2 (R&D Systems #AF2418 ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid for production of (His)6-SUMO-σAntA-DD was generated by site-directed mutagenesis (Agilent QuikChange) using primers listed in Table S3 ...
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bioRxiv - Cancer Biology 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). An equimolar pool of the different samples was sequenced on the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Genomics 2023Quote: ... Size and concentration of the finished Hi-C libraries was assessed by DNA ScreenTape Analysis (Agilent #5067-5582) on an Agilent 2200 Tapestation.
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bioRxiv - Genomics 2023Quote: Quality and quantification of the Hi-C libraries was assessed using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). Four biological replicates have been pooled in an equimolar manner and subjected to sequencing using the Illumina NextSeq 550 System in a 75bp paired-end setup ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNAs were obtained and amplified by high-fidelity RT-PCR using the AccuScript Hi-Fi (Agilent Technologies) reverse transcriptase and Phusion DNA polymerase (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-Fibronectin (Dako, 1:1000) in Block solution (4% BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-PCNA (Dako #M0879, 1:1000), anti-GFP (Abcam #ab13970 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-GFAP (1:1000, DAkoCytomation), rabbit anti-Iba1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-GFAP (1:1000; Agilent), mouse anti-Tuj1(1:100-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-S100β (1:1000; DAKO; Z0311) were diluted in PBTA and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat (1/1000, Dako), goat anti-rabbit (1/200 ...
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bioRxiv - Neuroscience 2020Quote: ... or GFAP (DAKO, 20334, 1:1000). The sections were then incubated for one hour with a goat anti mouse AlexaFluor 488 conjugated secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP (1:1000; Dako cat# Z0334), Iba1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit anti-S100 (Dako, 1:1000) or Guinea Pig anti-CK20 (Origene Tech ...
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bioRxiv - Bioengineering 2020Quote: ... GFAP (1:1000; Dako; Z-033429), or Iba-1(1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... using the DNA 1000 kit (Agilent). Multiplexed libraries (10ρM ...
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bioRxiv - Neuroscience 2023Quote: ... anti-C1Q (Dako A0136; 1:1000), anti-beta Actin (D6A8 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-GFAP (1/1000; Dako Z0334), anti-GALC (1/500 ...