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Citations for Agilent :
551 - 600 of 2353 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The amplicon was ligated in EcoRI/XhoI-digested pCMV-Tag 5A (Stratagene) to create pKozak-PDZD8-myc.
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bioRxiv - Evolutionary Biology 2023Quote: ... The mutant M1237I spike related constructs were generated using the QuikChange II site-directed mutagenesis kit (Agilent) with the primer set of S-M1237I-F ...
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bioRxiv - Biochemistry 2021Quote: Mutagenic PCR was used to introduce point mutations in both PgDHAR and HsCLIC1 (Table S2) following manufacturers protocol (Stratagene). 2-5μl of the PCR product was used to transform E ...
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bioRxiv - Cell Biology 2022Quote: ... organoids were incubated in blocking buffer containing primary antibody (rabbit anti-lysozyme 1:800, Dako #A0099) overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: ... resulting in 8 pairs of H&E and CK5 immunohistochemistry slides (Dako, catalogue number M7237 ...
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bioRxiv - Cell Biology 2022Quote: ... sh18.1 or sh18.4 were plated in E-plate 96 well (5232368001; Agilent) at a density of 20,000cells/well ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was performed using Accuscript Hi Fidelity First Strand Synthesis kit (Agilent). The amount of RNA entered into the reaction was normalised between samples ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Molecular Biology 2019Quote: pTrex-6×His-TOP1Y723F was generated by QuikChange II XL SDM kit (Agilent) using oligonucleotides 5’-CTAGGGTCCAGAAAATTGAGTTTGGAGGTTCCCAGG-3’ pTrex-6×His-TOP1 K117 ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 RBDL Nucleocapsid construct was expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were incubated in 0.1% Triton with 2% goat serum in PBS at 4°C overnight: rabbit anti-GFAP (1: 1000, DAKO, Z0334), rabbit anti-IBA-1 (1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3C library preparation and target enrichment using a custom-designed collection of 6979 biotinylated RNA “baits” targeting single MboI restriction fragments chr15:10283500-13195800 (mm9) (Supplementary Table S2; Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... a pool of the corresponding oligos (Tables S1 and S2) flanked by adapter sequences (TTCGATATCCGCATGCTAGC-tile-GATCGGAAGAGCACACGTCT) was synthesized (Twist or Agilent). Fragments were PCR-amplified (see Key resources table for primer sequences ...
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bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... listed in Table S2) and the site directed deletions and amplification were performed using Pfu turbo DNA polymerase (Agilent, #600250) following manufacturers protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants were prepared by site directed mutagenesis (see Tables S2 and S3 for details) following the QuikChange site-directed protocol (Agilent). For generation of knockin cell lines the pPLOT blast-mNG-blast plasmid was generated with recoded wild type and mutant profilin genes (synthetized by TWIST Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... The gene sequence was PCR-amplified with primers to introduce T7 promoters on both ends (see Table S2, Oligonucleotides tab) and cloned into pSC-A-amp/kan (Stratagene) vector ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed with primers (Table S2) and Brilliant Sybr Green QPCR Reagent Kit (Stratagene, Waldbronn, Germany). The ratio of G ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
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bioRxiv - Microbiology 2021Quote: ... coli XL1-Blue™ or SURE2™ Supercompetent Cells (Agilent, Germany) using QIAGEN Plasmid Mini and Midi Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... The Escherichia coli strain XL10-Gold (Agilent, Santa Clara, CA, USA) was used as a host for derivatives of plasmid pQE-30 ...
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bioRxiv - Biochemistry 2020Quote: ... coli XL-1 blue cells (Stratagene California, San Diego, CA, USA). For the cloning and ligation of the amplified gene ...
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bioRxiv - Biophysics 2021Quote: ACT was expressed in Escherichia coli XL-1 blue cells (Stratagene) transformed with pT7CACT1 plasmid ...
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bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2022Quote: ... DNA manipulations were carried out in Escherichia coli XL2 cells (Agilent). The proteins were expressed in E ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21-CodonPlus(DE3)-RIPL (Agilent Technologies, Santa Clara, CA, USA) cells were transformed with the resultant plasmids.
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bioRxiv - Biochemistry 2022Quote: ... Expression was performed in BL21(DE3)- RIPL Escherichia coli cells (Agilent) in LB at room temperature and induced through the addition of 0.2 mM IPTG for ~24 hours ...
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bioRxiv - Biophysics 2023Quote: ACT was expressed in Escherichia coli XL-1 blue cells (Stratagene) transformed with pT7CACT1 plasmid ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were transformed into Escherichia coli XL10-GOLD cells (Agilent), pEG103 construct transformants were selected using kanamycin (50 μg/mL in solid and 100 μg/mL in liquid medium ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21-Gold(DE3) competent cells (Agilent, Santa Clara, CA, USA). The expressions of TkSAHH and SfSAHH were performed in E ...
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bioRxiv - Immunology 2023Quote: ... coli strain BL21 (DE3) pLysS (Agilent Technologies, Santa Clara, CA, USA). The inclusion body containing the κCL was dissolved in 6M guanidine HCl ...
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bioRxiv - Biochemistry 2024Quote: ... His6-SUMO-ZF5.3 was transformed into Escherichia coli BL21DE3 Gold (Agilent) and selected on a kanamycin Luria Broth (LB ...
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bioRxiv - Cancer Biology 2019Quote: ... The quantification of RNA was performed by Thermo Scientific Nanodrop 1000 (Agilent) and Qubit 3.0 Fluorometer (LifeTechnologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used were: mouse anti-human CD8 (Dako, clone C8/144B, 1:800, pH 9 retrieval), mouse anti-human CD68 (Dako ...
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bioRxiv - Synthetic Biology 2022Quote: ... Absorbance spectra (300-800 nm) for the resulting product was analyzed on a spectrometer (Agilent, Cary 60) following purification and then following illumination with light from the ColorDyne Benchtop Lightsource at the wavelengths and periods of time indicated in S2 Fig ...
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bioRxiv - Physiology 2020Quote: ... 800 cardiomyocytes were plated in each well of XF24e Extracellular Flux Analyzer 24 well plates (Seahorse Bioscience) and mitochondrial respiration was assessed in 3 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... at a maximum pressure of 800 bar and chromotographically separated over the analytical column (Zorbax SB-C18, 1.8μm, 40cm x50μm; Agilent) using 90 min linear gradient from 7-30% solvent B (0.1% formic acid in acetonitrile ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Peak areas were integrated with MSD ChemStation software version E.02.01.1177 (Agilent Technologies). Peaks were identified by comparing their ion spectrum to the NIST library (NIST v2.2 ...
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bioRxiv - Plant Biology 2021Quote: ... Data processing was carried out by MSD Chemstation (E.02.02.1431, Agilent Technologies Inc.). Only VOC peaks with signal- to-noise ratios greater than 10:1 were considered for data quantification ...
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bioRxiv - Immunology 2020Quote: ... and backbone plasmid pNL4-3.Luc.R-E-(NIH AIDS Reagent) using GeneJammer (Agilent) in 10% FBS/DMEM enriched with 1% Pennicilin/Streptomycin (D10 medium) ...
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bioRxiv - Cell Biology 2023Quote: ... 15,000 cells/well were seeded in a 96-well E-plate (Agilent, 5232368001)47 containing gold microelectrodes fused to the bottom surface of the well plate ...
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bioRxiv - Biochemistry 2024Quote: ... All GC-MS parameters were controlled by ChemStation (G1701EA E.02.02.1431; Agilent Technologies), and data acquisition was completed within 24 h of derivatization ...
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bioRxiv - Genomics 2019Quote: ... The concentration of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies), and the Hi-C libraries were paired-end sequenced (HiSeq 2500 ...
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bioRxiv - Biochemistry 2021Quote: ... and the reaction was stopped by adding 2X GE HI-RPM hybridization buffer (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... the two elements were quantified in the bulk sediment-samples using an Agilent 240 AA flame-atomic absorption spectrometer (FAAS) (Agilent Technologies, Santa Clara, CA, USA). Sediment-samples were prepared following the United States Environmental Protection Agency’s prescribed method for acid digestion of sediments ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was sonicated using COVARIS S2 and peak fragment distribution between 100-500bp was verified on an Agilent Bioanalyzer (Agilent Technologies) using the DNA1000 chip ...
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bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated tetrameric spike (1ug) and RBD (0.5ug) were fluorochrome linked for flow cytometry by incubating with streptavidin conjugated APC (Prozyme) and PE (Prozyme ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...