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Citations for Agilent :
651 - 700 of 2353 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Genomics 2020Quote: ... Chromium Genome Reagents Kit Version 2 User Guide) and size and concentration determined using an Agilent 2100 Bioanalyzer DNA 1000 chip (Agilent Technologies, CA, USA). Libraries were then sequenced on an Illumina NovaSeq 6000 System following the manufacturer’s protocols (Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... at a maximum pressure of 800 bar and chromatographically separated over the analytical column (Zorbax SB-C18, 1.8 µm, 40 cm × 50 µm; Agilent) using 90 min linear gradient from 7-30% solvent B (0.1% formic acid in acetonitrile ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... MG and TA99 were used at a concentration of 1µg/ml together with a rabbit anti-human Von Willebrand Factor (1:800, Dako) in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... at a maximum pressure of 800 bar and chromatographically separated over the analytical column (Zorbax SB-C18, 1.8 μm, 40 cm × 50 μm; Agilent) using 90 min linear gradient from 7-30% solvent B (0.1% formic acid in acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... we conducted successive runs using two spectrophotometer microplate readers (models SYNERGY HTX and 800 TS; Agilent, Santa Clara, CA) simultaneously ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Neuroscience 2020Quote: The DNA from the patient and his parents was hybridized on a CGH platform (Agilent Technologies). The derivative log ratio spread (DLRS ...
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bioRxiv - Immunology 2022Quote: ... were introduced into pTT3-gH-His using the QuickChange XL II Kit (Agilent, Cat. number #200521) according to manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
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bioRxiv - Bioengineering 2023Quote: High-performance liquid chromatography (HPLC) (Shimadzu Co.) with an ion exchange column (Agilent Hi-Plex Column) was employed to detect the metabolites of first part of pathway and the final product ...
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bioRxiv - Plant Biology 2020Quote: ... Mutant F161A was made using the Quick-Change II-E site-directed mutagenesis kit (Stratagene) and a pair of complementary primers (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2021Quote: ... and biotinylated anti-rabbit IgG antibody which is produced in goats (E 0432-1 Dako Cyt ...
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bioRxiv - Biophysics 2021Quote: ... The MutL (R-E) mutation was generated using the QuikChange site-directed mutagenesis kit (Stratagene). Two serine residues separated the his6 and srt ...
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bioRxiv - Cell Biology 2022Quote: ... The E-plate was engaged into the xCELLigence Real Time Cell Analysis (RTCA) Instrument (Agilent) and background measurement of the wells plus growth media was recorded before adding cells in 150 μL of media for each SLC condition (5 x 103 cells/well) ...
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bioRxiv - Systems Biology 2023Quote: ... HBMECs were grown to confluency in xCELLigence 96 PET E-plates (Agilent Technologies Cat# 300600900). On the day of the assay ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Immunology 2020Quote: ... The DNA sequences encoding the SCP-tags were added by QuikChange site-directed mutagenesis (Stratagene, USA) using pMMHa BPTI-19A vector as a template [23] ...
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bioRxiv - Immunology 2023Quote: ... C5AP and PRGA with streptavidin tag were immobilized on pre-equilibrated AssayMAP Streptavidin columns (Agilent Technologies). Columns were washed with 1XPBS and then 100 µl of IVIG (1µl of IVIG diluted with 1XPBS to a final volume of 100 µl ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:1000, Dako Z0334); IL1β (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... -GFAP (1:1000; Dako, Z0334), -NF68 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP (Dako Z0334, 1:1000), pGSK3β (Tyr216 ...
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bioRxiv - Neuroscience 2019Quote: ... tau (Dako A0024, 1:1000), Iba1 (Wako Chemicals 019-19741 ...
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bioRxiv - Developmental Biology 2022Quote: ... using DNA 1000 kit (Agilent). The multiplexed libraries (10ρM ...
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bioRxiv - Cell Biology 2020Quote: ... Vimentin (Dako M0725, 1:1000), Fibronectin (BD Biosciences 610077 ...
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bioRxiv - Cancer Biology 2020Quote: ... and library average size was determined by Agilent DNA 1000 Kit (Agilent Technologies). Libraries were pooled for single-read (1 × 50 bp ...
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bioRxiv - Physiology 2023Quote: ... and warfarin (Agilent, #PST-1000). All chemicals were dissolved with manufactures’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... S100B (1:1000, Z0311, Dako) and vimentin (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... GFAP (Dako Z0334, 1:1000), GAPDH (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... or CD3χ (1:1000; Dako) at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... S100 (1:1000; Z0311, DakoCytomation), human vimentin (1:4000 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit-GFAP (1:1000, Dako), Goat-IBA1 (1:1000 ...
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bioRxiv - Biophysics 2019Quote: ... An in-house modification to the pumping system allows faster equilibration of the pressures: a second Tri-scroll 800 pump (Agilent) was connected to the source region (with an Edwards SP16K connected to the front pumping line) ...
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bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
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bioRxiv - Biochemistry 2022Quote: ... for a 200 μL sample of water in a well of a 96-well microtiter plate using the microplate reader and an 800 μL sample of water in a 1 cm quartz cuvette using a UV−Vis-NIR spectrophotometer (Cary 7000, Agilent) in the equation below ...
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bioRxiv - Microbiology 2023Quote: ... quality and size distribution of the libraries with capillary electrophoresis using D1000 ScreenTapestation from Agilent (size range 100-800 bp) (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... The libraries were pooled and quantified between the range of 150 bp and 800 bp using a Fragment Analyzer (Agilent). Library pools were sequenced on an Illumina HiSeq X Ten System ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were propagated in Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, CA), after growth in Lysogeny broth (LB ...
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bioRxiv - Cell Biology 2019Quote: ... The fusion protein was expressed in BL21 Codon Plus (DE3) RIPL Escherichia coli (Agilent) using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen) ...
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bioRxiv - Systems Biology 2021Quote: ... For plasmid propagation chemically competent Escherichia coli XL1-Blue (Agilent Technologies, Santa Clara, CA) cells were used which were grown in lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transformed into Escherichia coli strain BL21-Codon-plus(DE3)-RIL (Stratagene). Bacteria was grown in LB broth in a shaker at 37°C until reaching the log phase (A600nm between 0.4 and 0.5) ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21-Codon-Plus (DE3) RIPL was purchased from Stratagene (La Jolla, CA, USA) and used for protein overexpression ...
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bioRxiv - Molecular Biology 2019Quote: DNA cloning was performed using chemically competent Escherichia coli XL-10 Gold (Agilent Technologies) grown in LB medium (5 g L−1 yeast extract ...
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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bioRxiv - Biochemistry 2021Quote: ... All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene) using an autoinduction protocol (Studier ...
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bioRxiv - Biochemistry 2021Quote: The KlNmd4 protein was expressed in Escherischia coli BL21(DE3) Gold strain (Agilent technologies) using pMG897 plasmid and 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Biochemistry 2022Quote: ... coli (e.g. Invitrogen TOP10, cat. no. C404003; Agilent Technologies XL-10, cat. no. 200315)
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bioRxiv - Biophysics 2022Quote: TRPV4 N-terminal constructs were expressed in Escherichia coli BL21-Gold(DE3) (Agilent Technologies) grown in terrific broth (TB ...