Labshake search
Citations for Agilent :
7201 - 7250 of 8233 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
-
bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
-
bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
-
bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
-
bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
-
bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
-
bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
-
bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
-
bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... 1μl of each sample was used for quantification and quality verification (free of rRNA) in a second round of Agilent Bioanalyzer analysis using 2100 Prokaryote Total RNA Nano kit (Agilent, Santa Clara, CA). Libraries were constructed using 5 μl of mRNA using the Ultra II Directional RNA Library Preparation Kit (Agilent ...
-
bioRxiv - Molecular Biology 2024Quote: ... And assessed for their size distribution and adapter dimer presence using the High Sensitivity Small Fragment DNF-477 Kit (Agilent, # DNF-477-0500) on a 48-channel Fragment Analyzer (Agilent ...
-
bioRxiv - Neuroscience 2024Quote: ... quality check and quantification of total RNA was done using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 pico kit (Agilent Technologies, 5067-1513). Total RNA library preparation was done by introducing 500 ng total RNA into Illumina’s NEBNext Ultra II directional mRNA (UMI ...
-
bioRxiv - Molecular Biology 2024Quote: ... BRD2-BD mutant (N156A/N429A) and BRD3-BD mutant (N116A/N391A) were made using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies 200517-4). The BRD4-BE mutant was modified from BRD4-BD to harbor E651A/E653A point mutations.
-
bioRxiv - Immunology 2024Quote: ... The middle and lower lobes of the right lung were snap-frozen and then homogenized under liquid nitrogen for RNA extraction using the RNA isolation Kit (Agilent Technologies, TX, USA). The RNA analysis was carried out via standard RT-qPCR employing Bio-Rad SSO advanced Universal Probes Supermix (CA ...
-
bioRxiv - Neuroscience 2024Quote: ... the final PCR step has been modified using the KAPA HiFi HotStart Ready Mix.The cDNA libraries were quantified using Fragment Analyzer 5300 (Advanced Analytical) kit FA-NGS-HS (Agilent, Cat#: DNF-474-1000) and Spectramax M2 (Molecular Devices ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Mitochondrial respiration was assessed by measuring the oxygen consumption rate (OCR) using the Seahorse XFe24 flux analyzer and XF Cell Mito Stress test kit (Agilent, Santa Clara, CA) and according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... BG505 Env mutants and additional JR-CSF Env mutants were generated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, Santa Clara, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2024Quote: ... The RNA quality was checked by Trinean Xpose (Trinean NV, Gentbrugge, Belgium) and Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). rRNA was removed from total RNA with Ribo-Zero rRNA Removal Kit (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: ... T9A2-APOE4 same empty vector as reference was measured by using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, 103015-100) and Seahorse XFe96 FluxPak (Agilent Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... The quality and composition of the extracted RNA were assessed using the Agilent 2100 Bioanalyzer system with the RNA 6000 Pico Kit (Agilent, Santa Clara, USA). Ribosomal RNA (rRNA ...
-
bioRxiv - Cancer Biology 2024Quote: ... The integrity and quality of RNA were assessed on an Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies, CA, USA). Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 µg of plasmid ASAP3 and ASAP3-R3 were added into the solution of GeneJammer (Agilent) transfection reagent in Opti-MEM (3 µL in 200 µL) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, Dako, Japan) and HRP goat anti-rat IgG antibody (Biolegend ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were permeabilized using 0.2% Triton X-100 (Fisher) in 1:10 dilution Blocking Buffer (Dako) for 30 minutes at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... then stained with: rabbit polyclonal (pAb) glial fibrillary acidic protein (GFAP,1:1000, Dako, Z033401-2), goat pAb glial fibrillary acidic protein (GFAP,1:300 ...
-
bioRxiv - Molecular Biology 2020Quote: ... of which 1 μl was examines on a Bioanalyser high sensitivity DNA chip (Agilent 5067-4626). Ideal cycle number should bring final library to final concentration of 1-3 nM ...
-
bioRxiv - Molecular Biology 2021Quote: ... of which 1 μl was examined on a Bioanalyser high sensitivity DNA chip (Agilent 5067-4626). Ideal cycle number should bring final library to final concentration of 1-3 nM ...
-
bioRxiv - Molecular Biology 2020Quote: ... US Biological Life Sciences) and secondary antibody Rabbit Anti-Goat Ig/HRP (1:5,000, #P0160, Dako). Immunoblotting for α-Tubulin was performed using polyclonal Rabbit Anti-α-Tubulin antiserum as primary antibody (1:4,000 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies and dilutions used are as follows: guinea pig anti-insulin (DAKO, A0564, 1:1000), mouse anti-glucagon (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-PALB2 full-length and fragments were purified from 1 L of ArcticExpress cells (Agilent Technologies), grown at 37°C in LB broth medium containing 50 µg/mL ampicillin and 25 µg/mL gentamycin ...
-
bioRxiv - Physiology 2020Quote: ... BrdU positive cells were detected with an anti-BrdU primary antibody diluted 1:100 (DAKO Cytomatation) and with an anti-mouse-Alexa488 conjugated diluted 1:100 (Thermofisher) ...
-
bioRxiv - Biochemistry 2020Quote: ... Secondary antibody was polyclonal goat α rabbit IgG HRP conjugate (Dako, lot 20066477, 1:1000 dilution). ECL (Amersham Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... This was followed by incubation with the primary antibody (diluted 1:6,000 in dilution buffer; Dako) overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... NUP210 primary antibody (Atlas) was applied at a dilution of 1:100 in antibody diluent (Dako) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used in multispectral IHC were panCytokeratin (clone AE1/AE3, Dako®, dilution 1: 100), CD68 (clone M0876 ...
-
bioRxiv - Cell Biology 2021Quote: ... or HL-1 cells were seeded on 0.1% gelatin-coated XF24 cell culture microplates (Seahorse Bioscience) at 5 × 104 cells/well and incubated at 37°C in 5% CO2 overnight ...
-
bioRxiv - Plant Biology 2020Quote: ... equipped with a 80/100 alumina column (1/8” x 2 mm x 1.5 m, Agilent) and set with the following parameters ...
-
bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 hours in biotinylated swine anti-rabbit secondary antibody (1:200, Dako) followed by 2-hour incubation in Vectastain ABC (avidin-biotin ...
-
bioRxiv - Biochemistry 2022Quote: ... and aSN (primary antibody: anti-Syn-1, BD Transduction Laboratory, secondary antibody: anti-mouse-HRP, Dako). The interaction between endogenous aSN and the endogenous PMCA was studied in the extracts from C57BL/6 mice as described above.
-
bioRxiv - Developmental Biology 2022Quote: Primary antibodies used for staining of hBVOs were mouse anti-hCD31 (dilution 1:100, Dako, M0823), rabbit anti-hPDGFR-β (dilution 1:100 ...
-
bioRxiv - Cell Biology 2022Quote: ... NSPCs where incubated for 1 h in a non-C02 but humidified 37 °C incubator (Agilent) in the same XF assay medium as described above ...
-
bioRxiv - Immunology 2020Quote: ... Thermo Fisher MA USA) followed by SA-HRP (1:2000 dilution, #P030701-2, Dako, CA USA), and visualised with TMB (#421101 ...
-
bioRxiv - Microbiology 2020Quote: ... The cell proliferation marker Ki-67 was detected with mAb MIB-1 (Dako, Agilent Technologies, USA). For Ki-67 and sLea antigens ...
-
bioRxiv - Microbiology 2020Quote: ... The cell proliferation marker Ki-67 was detected with mAb MIB-1 (Dako, Agilent Technologies, USA). For Ki-67 and sLea antigens ...
-
bioRxiv - Cancer Biology 2020Quote: ... BCWM.1 cells were plated on 96 well cell culture (XFe96, Agilent Technologies, Santa Clara, USA) which had been precoated with CellTak (Thermo Fisher Scientific ...