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Citations for Agilent :
7101 - 7150 of 7497 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections (50 μm thickness) were prepared by a microslicer (DTK-1000, Dosaka EM) and treated with 1 mg/mL pepsin (DAKO) in PBS containing 0.1% Triton X-100 and 0.2 N HCl (Wako ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... 1:5,000 anti-mouse (for anti-FLAG) or 1:5000 anti-rabbit (for all the others) linked to peroxidase (Dako Agilent), and vizualized thanks to Clarity™ Western ECL substrate chemiluminescence reagent (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... Tissue samples were stained with hematoxylin and eosin or immunostained to detect SARS-CoV-2 antigen using VIDO-InterVac’s polyclonal rabbit anti-SARS-CoV-2 AS20-014 (1:400 dilution) and detected using an HRP-labelled polymer detection reagent (EnVision+ System – HRP Labelled Polymer, Agilent Technologies Canada Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... sections were incubated for 2 hours at room temperature with polyclonal guinea pig anti-insulin antibody (1:5, Agilent Technologies). Slides were then washed 3 x 5 minutes with PBS and incubated with the Anti-guinea pig IgG Alexa Fluor 488 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended by 200 μL 1% (w/v) BSA in PBS for detection by a NovoCyte flow cytometry (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Cancer Biology 2021Quote: ... while pH = 9.0 was used for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Biochemistry 2022Quote: ... and meprin α R372T (100 μl at 0.44 mg ml−1) were applied onto an AdvanceBio SEC column (300 Å, 7.8×300 mm, Agilent Technologies). The data was evaluated using ASTRA® 6 (Wyatt Technology).
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bioRxiv - Molecular Biology 2022Quote: ... the blots were further incubated with a primary anti-total Tau antibody (rabbit Dako-A0024, 1:5000 in blocking buffer), then with the anti-rabbit HRP-conjugated secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Physiology 2022Quote: ... Membranes were washed with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: swine anti-rabbit Ig HRP-conjugated (1:3000, catalog no. P0399, Dako) or goat anti-mouse Ig HRP-conjugated (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed three times with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibodies (Dako). Proteins were detected by enhanced chemiluminescence reagents (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The plasma (50 µL) was diluted with 150 µL of buffer A (1:4 dilutions, as recommended by Agilent Technologies), and then filtered to remove particulates using a 0.45 μm spin filter (Spin-X centrifuge tube filter ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Extracts precipitated using Mouse anti-Menin antibody were probed using Rabbit anti-GFAP antibody (Dako Agilent Technologies, #Z033429, 1:2000) whereas extracts precipitated with Rat anti-GFAP antibody were probed with Rabbit anti-Menin antibody (Bethyl Laboratories ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were incubated in 0.1% Triton with 2% goat serum in PBS at 4°C overnight: rabbit anti-GFAP (1: 1000, DAKO, Z0334), rabbit anti-IBA-1 (1 ...
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP or ready-to-use goat antirabbit HRP labelled polymer (Dako). The slides were washed twice in 1x Wash buffer and the color was developed with 3,3-diaminobenzidine tetrahydrochloride (DAB ...
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bioRxiv - Immunology 2020Quote: ... and were used directly in experiments or fractionated into CD45RA+ and CD45RO+ subsets using anti-CD45RA-PE (UCHL-1, Dako) and anti-PE magnetic beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... SIV vpx start codon and two additional ATG codons upstream of the HIV-1 vpr start codon were mutated by Quickchange mutagenesis (Stratagene) and the sequence between the stop codon of vif and start codon of vpr were deleted ...
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bioRxiv - Molecular Biology 2020Quote: ... Converted libraries were enriched by 10 cycles of PCR with the following reaction composition: 1 μl Pfu TurboCx Hotstart DNA polymerase (Stratagene), 5 μl PfuTurbo Cx reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secondary antibodies used were goat anti-rabbit BrightVision Horse Radish Peroxidase (Klinipath, Duiven, The Netherlands) and goat anti-mouse (#A32723, dilution 1:5000) obtained from Dako products (CA ...
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bioRxiv - Neuroscience 2021Quote: ... we used horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (diluted 1:200 in PBS; DAKO); staining for fluorescent immunohistochemistry was performed using donkey anti-mouse ...
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bioRxiv - Microbiology 2021Quote: ... 1 µL was injected in splitless mode through a GC inlet liner (ultra inert, splitless, single taper, glass wool, Agilent) onto a DB-5MS column (30 m × 0.25 mm ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
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bioRxiv - Physiology 2022Quote: ... for 1 h at RT and incubated overnight at 4°C with 1:100 dilution of mouse anti-Ki67 antibody (Novocastra, Concord, ON, Canada) in antibody diluent (DAKO). Next ...
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bioRxiv - Biochemistry 2020Quote: UV-visible spectra were recorded in 1-cm optic path quartz cuvettes under aerobic conditions on a Cary 100 Uv-vis spectrophotometer (Agilent) and under anaerobic conditions in a glove box on a XL-100 Uvikon spectrophotometer equipped with optical fibers.
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bioRxiv - Synthetic Biology 2020Quote: ... 1 μL of this mixture was injected into an Agilent 7890A Gas Chromatograph with 5977A MSD (Agilent, Santa Clara, CA) using an Agilent HP-5MS (30 m length x 0.25 mm i.d ...
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Genomics 2021Quote: ... Between 1 and 5 ng of the purified digested DNA was loaded on a High Sensitivity DNA chip (Agilent Technologies) and run on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Neuroscience 2020Quote: ... we used horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse secondary antibodies (diluted 1:200 in PBS; DAKO) to bind the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Stained cells suspensions were counted in 20 μl sample volume at medium flow rate (60 μl min−1) using an ACEA NovoCyte Green flow cytometer (OMNI Life Science Agilent). The SYBR Green I signal was measured in the FITC-channels of the instrument ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were thoroughly washed with 0.3% Triton-TBS followed by antigen retrieval with target retrieval solution citrate pH6 (1:10, Dako S2031) for 20 minutes at 80°C ...
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bioRxiv - Systems Biology 2020Quote: ... lymphatic tissue was cut into 1-mm pieces with a sterile scalpel and disaggregated mechanically into a single-cell suspension using the Medimachine (DAKO) equipped with sterile cartridges holding rotating knives (Medicons ...
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bioRxiv - Zoology 2020Quote: ... Slides were rinsed with PBS (pH = 7.4) and then subjected to antigen retrieval procedure using proteinase K 1:30 (Dako, Denmark). Nonspecific binding sites were blocked with 2% BSA for 30 min in a humid chamber at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected with a DNA mixture containing either the 4xNFAT or the 7xAP-1 promoter-Firefly luciferase reporter (100 ng; Stratagene), along with Renilla luciferase (50 ng of pGL4.70[hRluc] or 1 ng of pRL-null ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and incubated with biotinylated rabbit anti-rat IgG for 1h (1:200, DAKO Cytomation A/S, Denmark). Following avidin-biotin-peroxidase complex (Vector laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... 25 and 50 ppb with Sc-45 (ICP-MS internal standard mix 1 ug/mL in 2% HNO3, Agilent Technologies) as the internal standard for Cu-63.
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bioRxiv - Developmental Biology 2021Quote: ... Half of the cDNA was amplified for 17 PCR cycles and a 1:4 dilution of the resulting library was assessed by Agilent Bioanalyzer High Sensitivity DNA kit ...
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bioRxiv - Plant Biology 2021Quote: ... The filtrate was passed through a 1 g phase C18 silica-bonded non-polar column (HF Mega Bond Bond Elut C18, Agilent, 5301 Stevens Creek Blvd ...
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bioRxiv - Developmental Biology 2021Quote: ... and the sections were incubated for 1 h at room temperature with biotinylated IgG anti-mouse secondary antibody which is produced in rabbit (E 0354 -Dako Cyt ...
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bioRxiv - Genomics 2021Quote: ... and a second 1 μl aliquot was used to determine molecule length with an Agilent Genomic Tape (Agilent, Cheadle, UK) on an Agilent TapeStation (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation for 45 minutes at room temperature in 1:100 dilution of rabbit anti-goat immunoglobulins/HRP (Dako, P0160 or ready-to-use goat anti-rabbit HRP labelled polymer (Dako ...