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Citations for Agilent :
7401 - 7450 of 8233 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: We performed in-solution targeted enrichment of exonic sequences from spleens of two heterozygous MVD013 mice on C3H background together with six mutants and two wild-type controls from three other lines on the same genetic background using the SureSelectXT Mouse All Exon 50 Mb kit (Agilent Technologies, Santa Clara, CA). The generated libraries were indexed ...
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bioRxiv - Immunology 2022Quote: ... and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Cell Energy Phenotype kit on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The quality of extracted RNA was verified using the Agilent 2100 Expert Bioanalyzer using the RNA 1000 Nano kit (Agilent Technologies, Santa Clara, CA). Ribosomal RNA depletion and library construction were performed using the RiboZero Plus kit and TruSeq stranded mRNA library preparation kit (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Basal oxygen consumption rate and mitochondrial Oxidative Phosphorylation System (OXPHOS) parameters were measured according to manufacturer’s instructions of the Mito Stress test Kit (Seahorse Bioscience, Agilent Technologies, CA, USA). Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... RNA quality was determined by spectrophotometry at 260 nm and 280 nm and by fluorescence capillary assay (RNA 6000 Pico kit, Agilent Technologies, Santa Clara, Ca). RNA integrity numbers ranged from 2.3 to 6.2.
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bioRxiv - Physiology 2022Quote: ... and concentration prior to library generation with the Agilent RNA Pico 6000 kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... SCR_019417) was used for library size distribution analysis with a high-sensitivity NGS Fragment Kit (Cat# DNF-474-0500, Agilent, Santa Clara, CA, USA). qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A subset of the samples from each developmental stage was quality-checked by Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies Catalog # 5067-1513). The resulting RNA integrity numbers were between 8.4 and 10 ...
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bioRxiv - Genomics 2023Quote: ... The quality of the cDNA libraries and final DNA libraries were analyzed using the 2100 Bioanalyzer Instrument with the High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA).
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bioRxiv - Pathology 2023Quote: ... RNA concentration was determined using an RNA 6000 Pico Kit run on a Bioanalyzer 2100 running Agilent 2100 Expert Software (B.02.11; Agilent Technologies, Santa Clara, CA, USA). RNA was converted to cDNA using iScript cDNA Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of RNAt per sample were separated and their concentrations adjusted to 50 ng μL−1.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Point mutation of D5R-STOP from D5-TANGO was made by polymerase chain reaction (PCR) using the QuikChange II Site–Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Point mutations were induced using either the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... the cDNA was purified by SPRIselect Reagent (Cat# B23318) and quantified using Picogreen™ dsDNA assay kit (Cat# P7589) and quality assessed using High Sensitivity D5000 ScreenTape (Agilent, Cat# 5067-5592) with High Sensitivity D5000 Reagents (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of total RNA (RNAt ...
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bioRxiv - Immunology 2024Quote: RNA from splenocytes isolated from UVB exposed WT and Trim21-/-mice were analyzed for RNA integrity on the 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA) and quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting library pools then underwent quality and quantity measurements via electrophoresis (High-Sensitivity DNA Kit and Agilent Bioanalyzer; Agilent Technologies, Santa Clara, CA, USA), real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... RNA quantity and integrity were measured with an Agilent 2100 bioanalyzer using the RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA) and by agarose gel electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... The quality of extracted RNA was verified using the Agilent 2100 Expert Bioanalyzer using the RNA 1000 Nano kit (Agilent Technologies, Santa Clara, CA). Ribosomal RNA depletion and library construction were performed using the RiboZero Plus kit and TruSeq stranded mRNA library preparation kit (Illumina ...
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bioRxiv - Genomics 2024Quote: Metabolic measurements of HAEC were performed with Seahorse XF Mito Stress Test and XF Glycolytic Rate Assay kits using a Seahorse XFe96 Analyzer (Agilent Technologies, Santa Clara, US). 50,000 endothelial cells were seeded into the Seahorse 96-well plates and were treated with 50ug/mL oxLDL or control buffer 24 hours prior to the assay ...
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bioRxiv - Neuroscience 2024Quote: ... The library quality was evaluated by measuring the library size in base pairs using the Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). During the library construction ...
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bioRxiv - Cell Biology 2024Quote: ... Sample amplification and labeling were carried out using the Low Input Quick Amp labeling Kit (Catalog number 5190-2305, Agilent Technologies, Santa Clara, CA). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... GST-Rab5C213A and GST-Rab5C212A/C213A in pGEX-2T were constructed by mutating GST-Rab5 (wild type) in pGEX-2T using a Quik-Change Site-Directed Mutagenesis Kit (Agilent Technologies, San Diego, USA). The mutated Rab5 genes were then cloned into the pCMV-HA vector ...
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bioRxiv - Cell Biology 2024Quote: ... The expression vector for the A27F mutant of M2 was obtained by PCR-based mutagenesis using a QuickChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA) and with primers:
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bioRxiv - Genetics 2024Quote: Microdeletions and candidate variants (Supplementary Table S1) were introduced into the wild type (WT) construct by site-directed mutagenesis using the QuikChange Lightning Kit (Agilent, Santa Clara, CA, USA). Mutant minigenes were confirmed by sequencing (Macrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... We used the following antibodies for the murine sections: anti-GFAP (1:500, Dako, raised in mouse), anti-NeuN (1:150 ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with rabbit anti-mouse horseradish peroxidase (HRP) conjugated antibody (P026002-2, Dako, 1:1000) as secondary antibody at RT for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... the plasma was diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies) and filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary incubations were performed with horseradish peroxidase-linked polyclonal goat anti-rabbit (1:5000; Dako, Glostrup, Denmark) or polyclonal rabbit anti-mouse (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with secondary horseradish peroxide (HRP)-conjugated goat anti-rabbit antibodies (Dako P0448, 1:1000) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... and covered with glass coverslip of #1 thickness (Carl Roth GmbH NK79.1) using mounting media (Dako S3023). The sections were imaged on Zeiss LSM 780 confocal microscope ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were incubated with primary antibodies diluted in blocking buffer: anti-CD31 (1:50; Agilent Technologies #M0823) and anti-CD146 (1:600 ...
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bioRxiv - Physiology 2021Quote: ... 1:100 dilution) antibodies were diluted in DAKO primary antibody diluent (Agilent Technologies, Santa Clara, CA, USA) and applied to sections overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... rabbit polyclonal anti-CD3 antibody at a dilution of 1:200 (A045229-2, Dako Agilent Pathology Solutions), rat monoclonal anti-CD45 antibody at a dilution of 1:100 (05-1416 ...
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bioRxiv - Microbiology 2020Quote: ... rabbit polyclonal anti-CD3 antibody at a dilution of 1:200 (A045229-2, Dako Agilent Pathology Solutions), rat monoclonal anti-CD45 antibody at a dilution of 1:100 (05-1416 ...
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bioRxiv - Microbiology 2021Quote: ... Sample volumes of 1 mL were injected into an Agilent 7890 RGA Gas Chromatograph (Agilent Technologies, USA). A flame ionisation detector determined C1–C5 hydrocarbon gas concentrations that were used to calculate gas wetness ...
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bioRxiv - Microbiology 2021Quote: ... incubated in a 1:2000 dilution of peroxidase-conjugated anti-rabbit immunoglobulin (Dako, Santa Clara, California, USA) in PBS with 3% skimmed milk and 0.05% Tween for 45 min. ...
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bioRxiv - Neuroscience 2022Quote: ... They were then incubated overnight at +4 °C with the 6F3D anti-Aβ antibody (Dako, 1/200), polyclonal anti-tau antibody (Dako ...
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bioRxiv - Synthetic Biology 2022Quote: ... Both systems contained a DB-VRX capillary column (20 m, 0.18 mm ID, 1 µm film; Agilent) and a 100 µL gastight syringe (G4513-80222 ...
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bioRxiv - Genomics 2022Quote: ... into 1 mL 96 well plates and sealed with a silicone plate mat (Agilent, Santa Clara, CA). Aliquots of 12 samples from each row were combined into pools ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μl sample was injected into a gas chromatograph coupled to a mass spectrometer (GC-MS; Agilent) on a HP-5MS column (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... FcR binding was quantified by incubating immune complexes with biotinylated FcRs (FcγR2A-1, FcγR2A-2, FcγR3A, courtesy of Duke Protein Production Facility) conjugated to Steptavidin-PE (Prozyme). Flow cytometry was performed with an IQue (Intellicyt ...
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bioRxiv - Immunology 2020Quote: ... consistently identified GFAP peptides while minimizing nonspecific off-target peptide identification (Agilent Z033429-2, 1:200 dilution). Each peptide was required to have a minimum of 1 RPK as well as a FC > 10 ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: The following primary antibodies were used (with dilution): Gfap (Rabbit polyclonal, 1:2500, Dako/Agilent Cat# Z0334), C3 (Rat monoclonal ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: The following primary antibodies were used (with dilution): Gfap (Rabbit polyclonal, 1:2500, Dako/Agilent Cat# Z0334), C3 (Rat monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were then incubated for 45 min with Polyclonal Anti-Mouse 1:100 (P0447, Dako-Agilent) or a BrightVision Poly-HRP-Anti Rabbit IgG ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were then incubated for 45 min with Polyclonal Anti-Mouse 1:100 (P0447, Dako-Agilent) or a BrightVision Poly-HRP-Anti Rabbit IgG ...
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bioRxiv - Microbiology 2021Quote: ... and the average library size (Supplementary Table 1) was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The library was diluted (to 6.5 pM ...