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Citations for Agilent :
7151 - 7200 of 7497 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... MCF10A shCtrl and shEIF3E cell lines were transfected in 100 mm tissue culture plates with 5 μg of DNA at a ratio of 1:50 of a T7-tagged PRP4K expression vector (16) or pBluescript SK-(Stratagene/Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and RNA quality and integrity was assessed though gel electrophoresis (1% agarose gel) and the Agilent Bioanalyzer 2100 (Agilent Technologies). Labelling and hybridization were performed ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were passed through an immobilized pepsin column (2mm 2cm) at 200 μl min−1 and the digested peptides were captured on a 2mm 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Microbiology 2021Quote: Immunofluorescent (IF) staining was performed using the following primary antibodies: polyclonal rabbit anti-HSV-1 (cross-reactive with HSV-2; Agilent), anti-Iba1 (Wako ...
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bioRxiv - Neuroscience 2020Quote: Total lysate and synaptoneurosomes isolated from cortex tissue of 1-month-old C57Bl/6 mice were UV crosslinked (100 mJ/cm2 for 2 cycles) using UV Stratalinker 2400 (Stratagene) and stored at −80°C until use ...
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bioRxiv - Genomics 2022Quote: ... and 1 μl was removed to assess tagmentation fragment size and yield by D5000 ScreenTape (Catalog No. 5067-5588, Agilent) on an Agilent Tapestation.
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for 2-days in complete RPMI to allow for attachment to the culture dish and then for 1-hour in XF RPMI medium (Agilent) with 2.8 mM glucose ...
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bioRxiv - Biochemistry 2022Quote: ... Astrocytes were pre-incubated with 10 μM CGP-37157 or DMSO for 1 h and then plates were inserted in a XFe24 Seahorse Analyzer apparatus (#102238-100, Agilent). Cells were stimulated with 100 μM ATP ...
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bioRxiv - Biophysics 2022Quote: TTP was run on a tandem size exclusion chromatography - multi-angle light scattering detector by injecting 100 μL sample at a concentration of 1 mg/mL using a 1260 Infinity HPLC (Agilent). The column was pre-equilibrated in 0.1 μm filtered buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were acidified by 1% trifluoroacetic acid (TFA) and C18 cleanup was performed using Bravo AssayMAP robot with C18 cartridges (Agilent) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively, 1:5000 for Western blot). The rabbit polyclonal anti-FBP17 (1:1000 for Western blot ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently rinsed with PBS supplemented with 0.01% Tween 20 and probed with horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (1 in 500 dilution) (DAKO, Agilent Technologies) for 40 min ...
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bioRxiv - Plant Biology 2022Quote: ... adsorption at 45°C for 5 minutes and desorption at 250°C for 1 minute onto a DB-624 capillary column with 60m x 0.25mm x 1.40μm film thickness (Agilent Technologies, Böblingen, Germany). The GC temperature was programmed as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... we used horseradish peroxidase (HRP)-conjugated isotype-specific anti-rabbit IgG or anti-mouse IgG (1:10000; DAKO, Cambridgeshire, UK) secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Bone marrow was flushed out using a syringe and 1 ml of PBS + 5% FBS and filtered through a 40 μm strainer (Dako). Cells were counted and analyzed immediately ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with a goat anti-rabbit HRP-conjugated secondary antibody (Dako P0448, 1:2000 dilution in 5% BSA-PBST) for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated at 37°C for 1 h before the plate was placed into an Extracellular Flux Analyzer XFe96 (Seahorse, Agilent) for measuring the extracellular acidification rate (ECAR ...
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bioRxiv - Biochemistry 2022Quote: ... for a 200 μL sample of water in a well of a 96-well microtiter plate using the microplate reader and an 800 μL sample of water in a 1 cm quartz cuvette using a UV−Vis-NIR spectrophotometer (Cary 7000, Agilent) in the equation below ...
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bioRxiv - Plant Biology 2024Quote: ... 500 mg (1 mL) of the powder was transferred immediately to a 20 mL head-space vial (Agilent, CA, USA), containing NaCl saturated solution ...
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bioRxiv - Systems Biology 2024Quote: ... and detected by 1:2000 dilution of respective secondary HRP-antibody-conjugates (anti-rabbit, P044801-2, Agilent; anti-mouse, P044701-2, Dako).
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bioRxiv - Neuroscience 2024Quote: ... The cells were washed once with DMEM Assay Medium (XF DMEM [Agilent] supplemented with XF glucose [10 mM, Agilent], XF glutamine [2 mM, Agilent] and XF pyruvate [1 mM, Agilent]), then left in DMEM Assay Medium and placed in a non-CO2 incubator for 1 hour ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Microbiology 2024Quote: The frozen samples were quickly thawed and injected into a refrigerated (1°C) HPLC system (Agilent Infinity 1260, Agilent Technologies), through a porcine pepsin column (≥ 3200 units/mg ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-GGCT (16257-1-AP; proteintech) antibodies were used for immunodetection on rehydrated sections pretreated with Target Retrieval Solution (S1699; DAKO). The Envision™+ system (HRP/DAB ...
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bioRxiv - Molecular Biology 2024Quote: ... quenched samples were passed through a co-immobilized pepsin/fungal XIII protease column (1mm × 2cm) at 50 µl min−1 and the digested peptides were captured on a 1mm × 1cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed overnight at 4 °C with rabbit monoclonal anti-PSyn EP1536Y diluted 1:320,000 in DAKO antibody diluent (Agilent #S0809). After three 5-min washes with PBST ...
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bioRxiv - Cell Biology 2024Quote: ... of WRN was amplified by PCR from the pCMV-FlagWRN plasmid or from the pCMV-FlagWRNS1133D mutant The PCR product were subsequently purified and sub-cloned into pGEX4T-1 vector (Stratagene) for subsequent expression in bacteria as GST-fusion proteins ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated at room temperature for 1 h with rabbit anti-mouse IgG (Agilent, P026002-2) or goat anti-rabbit IgG (Agilent ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Genomics 2024Quote: ... We selected for guide integration with 2µg/ml puromycin and then induced prime editor expression with 1 µM doxycycline and determined editing rates by visualizing amplicons (Supplementary Table 2) on the bioanalyzer (Agilent).
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Biochemistry 2024Quote: ... For all experiments the TIMS elution voltages were calibrated by known 1/K0 values from at least two out of three ions from Agilent ESI LC/MS tuning mix (m/z ...
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in a 0% CO2 chamber at 37 °C for 1 hour before being placed into a Seahorse XFe24 Analyzer (Agilent). For oxygen consumption rate (OCR ...
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bioRxiv - Neuroscience 2023Quote: ... The steroid extraction was performed as earlier described for plasma [39] with a further modification of the CSF analysis to encompass a different size of solid-phase extraction columns (100 mg Bond elute C18 solid-phase extraction cartridges; 1 ml; Agilent) and therefore corresponding volume changes for conditioning (1 ml MeOH followed by 2×1 ml dH2O) ...
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bioRxiv - Microbiology 2023Quote: ... skimmed milk powder and probed with desired primary antibodies and secondary horseradish peroxidase conjugated IgG antibodies at 1:5000 (Dako Agilent). Protein bands were detected using a G:BOX (Syngene ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were permeabilized with 0.2% Triton X-100 in 2% fish gel for 2 hours at room temperature and immunohistochemically labelled with the primary antibody (1:200 rabbit anti-GFAP, Z0334 Dako; 1:200 rabbit anti-Iba1 ...
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bioRxiv - Microbiology 2022Quote: We extracted extracellular metabolites from the seawater matrix using Bond Elut PPL cartridges (1 g/6 ml sized cartridges, Agilent) following the protocol of Dittmar et al ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal goat anti-rabbit secondary antibody IgG/HRP (P044801-2; Agilent Technologies/Dako ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated for 1 h at room temperature with polyclonal rabbit anti-mouse secondary antibody IgG/HRP (P0260; Agilent Technologies/Dako) diluted 1:2000 in 3% BSA.
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bioRxiv - Cancer Biology 2023Quote: ... ~100 separate PCR reactions (library and representation dependent) were performed with 1 μg genomic DNA in each reaction using Herculase II Fusion DNA Polymerase (Agilent) or Phusion High-Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200, GFAP from Agilent DAKO Z033429-2 at 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with either horse radish peroxidase (HRP)-labeled anti-rabbit IgG (1:20000, Agilent Dako, Poland) or HRP-labeled anti-mouse IgG (1:20000 ...
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bioRxiv - Developmental Biology 2023Quote: ... with a KpnI site inserted at the 5’ end of Δ3-1 or Δ3-2 (Quick Change Site-Directed Mutagenesis from Stratagene) using the oligonucleotides Δ3-1-KpnI Fw ...