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Citations for Agilent :
501 - 550 of 1245 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The plasmids were transformed into Escherichia coli strain BL21 (DE3) RIL (Stratagene). Expression was induced at 16 °C overnight with 0.2 mM of IPTG ...
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bioRxiv - Microbiology 2019Quote: ... coli strain BL21-CodonPlus (DE3)-RIL (Agilent Technologies, Santa Clara, CA, USA), and purified using HisTrap HP (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Protein expression was performed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Agilent). Bacterial cell cultures were grown in LB at 37°C to an OD600 of 0.6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cloning host Escherichia coli XL1-Blue strain was from Stratagene (USA), and the plasmid pRSETC and E ...
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bioRxiv - Biochemistry 2023Quote: Protein complexes were overproduced in BL21-CodonPlus (DE3) Escherichia coli cells (Agilent) grown in high salt LB Broth (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Electroporation-competent Escherichia coli TG1 cells (Agilent Technologies Japan, Ltd., Tokyo, Japan) were transformed with the ligated plasmids using the Gene Pulser instrument (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21-CodonPlus (DE3)-RIPL competent cells (Agilent, Santa Clara, CA, USA). LB medium was used for most seed cultures and main cultures ...
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bioRxiv - Microbiology 2023Quote: ... coli and functional MotA-ASRs through QuickChange Site-Directed Mutagenesis Kit (Agilent). Initially ...
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bioRxiv - Biochemistry 2023Quote: GBP2FL was overexpressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent). The cells were grown in LB broth containing 34 µg/ml chloramphenicol and 50 µg/ml kanamycin at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... coli BL21-Gold (DE3) chemically competent cells (Agilent Technologies, Santa Clara, CA). The expression and screening of iNicSnFR variants were performed in 96-well plate format ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Sections for SARS-CoV-2 IHC were blocked with 10% goat serum (X0907, DAKO, Agilent Technologies Netherlands B.V) for 30 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... Staining was developed using a rabbit anti-SARS-CoV serum and a secondary alexa-fluor-labeled conjugate (Dako). The number of infected cells per well were counted using the ImageQuant TL software.
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bioRxiv - Cancer Biology 2020Quote: ... 4µm sections were stained with H+E and by IHC for p53 (DAKO IS616), Pax8 (Abcam ab181054 ...
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bioRxiv - Neuroscience 2021Quote: ... and cover-slipped (Catalog #12-544-E) using fluorescence mounting medium (Dako, Cat. #S3023).
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bioRxiv - Molecular Biology 2022Quote: ... The resulting chromatograms were processed using the software MSD ChemStation E.01.00.237 from Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded (8000 cells/well) in a 96-well RTCA E-plate (Agilent) in 4 replicates per condition ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene). Melanoma cells were transfected with combinations of reporter constructs ...
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bioRxiv - Cancer Biology 2024Quote: Mahlavu and Huh7 cells were plated into E-96 plates (Agilent, cat. no:5232368001), 1500-2500 cells/wells ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was sequenced with IonTorrent using a 318 chip (v2) and internal ERCC Spike-In control after ensuring the cDNA quality (Agilent Bioanalyzer 2100 DNA High Sensitivity chip) ...
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bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2021Quote: ... Relative and quantitative expression in leaves and spikes was analyzed using Real-Time PCR Detection Machine (Stratagene Mx3005P, Agilent Technologies) and KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Biochemistry 2022Quote: ... All site-directed mutagenesis was performed on this codon-optimized spike clone as the WT form using the QuikChange II Site-directed mutagenesis kit (Agilent), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
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bioRxiv - Bioengineering 2022Quote: ... The Escherichia coli strains DH5α and BL21-Gold (DE3) were purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains from glycerol were further analyzed through HPLC (1100 Series HPLC; Agilent) connected with MS (LC/MSD VL ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli BL21-CodonPlus(DE3)-RIPL cells (Agilent Technologies, Santa Clara, CA, USA) were transformed with either pET15-MinD ...
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bioRxiv - Biophysics 2022Quote: ... His6-SUMO-TTL was expressed in Escherichia coli (BL21-DE3 competent cells, Agilent) overnight at 18 °C in LB broth (induced by 0.5 mM IPTG) ...
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bioRxiv - Biochemistry 2022Quote: ... Each construct was transformed into Escherichia coli BL21(DE3) Codon Plus Cells (Agilent). Select colonies were inoculated in 50 ml of Luria Broth (LB ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs were overexpressed in Escherichia coli BL21-gold (DE3) cells (Agilent Technologies) using ZYM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Genomics 2019Quote: ... The plasmid was transformed and expanded in XL10-Gold Escherichia coli strain (Stratagene). The cDNA of the plasmid was sent for Sanger sequencing to validate the gene sequence.
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bioRxiv - Plant Biology 2019Quote: ... and transformed into XL1-BLUE Escherichia coli competent cells (Stratagene, Santa Clara, USA). Three hundred and thirty-six clones (48 from each library ...
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bioRxiv - Biochemistry 2021Quote: ... A freshly transformed colony of Escherichia coli BL21-CodonPLus (DE3)-RIPL cells (Agilent) was inoculated into 100 ml of Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transformed into Eschericia coli BL21-CodonPlus (DE3)-RIL cells (Agilent Technologies) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells from Agilent and purified as previously described (Haizel et al. ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21-ArcticExpress (DE3)-RIL competent cells (Agilent Technologies, Santa Clara, CA, USA) in Terrific Broth modified medium (Melford ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Immunology 2023Quote: ... codon-optimized for Escherichia coli and synthesized as an oligomer pool (Agilent Inc). Each peptide contained an alanine/glycine N-terminus linker (AAVVGGV ...
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bioRxiv - Biophysics 2023Quote: ... and eIF4E were expressed and purified from Escherichia coli BL21(DE3) RIPL (Agilent) following established procedures30,35,40,41 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells (NEB 5-alpha: C2987, Agilent through UCB MacroLab XL1-Blue: 200249) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Plant Biology 2020Quote: ... Mutant F161A was made using the Quick-Change II-E site-directed mutagenesis kit (Stratagene) and a pair of complementary primers (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2021Quote: ... and biotinylated anti-rabbit IgG antibody which is produced in goats (E 0432-1 Dako Cyt ...
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bioRxiv - Biophysics 2021Quote: ... The MutL (R-E) mutation was generated using the QuikChange site-directed mutagenesis kit (Stratagene). Two serine residues separated the his6 and srt ...
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bioRxiv - Cell Biology 2022Quote: ... The E-plate was engaged into the xCELLigence Real Time Cell Analysis (RTCA) Instrument (Agilent) and background measurement of the wells plus growth media was recorded before adding cells in 150 μL of media for each SLC condition (5 x 103 cells/well) ...