Labshake search
Citations for Agilent :
451 - 500 of 1245 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... coli BL21-gold (DE3) cells (Agilent Technologies, Santa Clara, CA) using ZYM-5052 autoinduction media (Studier ...
-
bioRxiv - Biochemistry 2022Quote: ... coli XL10-Gold competent cells (Agilent Technology, Santa Clara, CA). The colonies formed on Luria–Bertani (LB ...
-
bioRxiv - Biophysics 2022Quote: ... coli BL21(DE3) cells (Agilent Technologies, Santa Clara, CA, USA) for protein expression.
-
bioRxiv - Neuroscience 2023Quote: ... coli strain BL21-CodonPlus(DE3)-RIL strain (Agilent Technologies, 230245). Cells were cultured at 37°C until OD600nm reached 0.5 ...
-
bioRxiv - Plant Biology 2023Quote: ... coli BL21-CodonPlus-RIPL [λDE3] from Stratagene (Santa Clara, CA). The pEC-GFP-CBM3a vector was kindly provided by Dr ...
-
bioRxiv - Cell Biology 2023Quote: Plasmid constructs were transformed into Escherichia coli BL21 DE3 (Stratagene) stains ...
-
bioRxiv - Biochemistry 2023Quote: ... coli ArcticExpress (DE3) cells (Agilent Technologies, Santa Clara, CA, USA) similar as previously described [5] ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21-CodonPlus (DE3)-RIPL cells (Agilent, CA, USA, #230280) transformed with the pET28a-based FLAG-Topbp1 expression plasmid by the addition of 0.5 mM IPTG in Terrific broth for 16 h at 25°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Vectors were transformed into BL21(DE3) competent Escherichia coli (Agilent) and a starter culture was grown at 37°C overnight in 10 ml Luria Broth (LB ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The mutant M1237I spike related constructs were generated using the QuikChange II site-directed mutagenesis kit (Agilent) with the primer set of S-M1237I-F ...
-
bioRxiv - Biochemistry 2021Quote: Mutagenic PCR was used to introduce point mutations in both PgDHAR and HsCLIC1 (Table S2) following manufacturers protocol (Stratagene). 2-5μl of the PCR product was used to transform E ...
-
bioRxiv - Pathology 2019Quote: ... resulting in 8 pairs of H&E and CK5 immunohistochemistry slides (Dako, catalogue number M7237 ...
-
bioRxiv - Cell Biology 2022Quote: ... sh18.1 or sh18.4 were plated in E-plate 96 well (5232368001; Agilent) at a density of 20,000cells/well ...
-
bioRxiv - Immunology 2020Quote: ... The RBD domain of SARS-CoV-2 S was biotinylated and tetramerized with streptavidin-APC (Agilent). The APC decoy reagent was generated by conjugating SA-APC to Dylight 755 using a DyLight 755 antibody labeling kit (ThermoFisher) ...
-
bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 RBDL Nucleocapsid construct was expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3C library preparation and target enrichment using a custom-designed collection of 6979 biotinylated RNA “baits” targeting single MboI restriction fragments chr15:10283500-13195800 (mm9) (Supplementary Table S2; Agilent Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... a pool of the corresponding oligos (Tables S1 and S2) flanked by adapter sequences (TTCGATATCCGCATGCTAGC-tile-GATCGGAAGAGCACACGTCT) was synthesized (Twist or Agilent). Fragments were PCR-amplified (see Key resources table for primer sequences ...
-
bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: ... The SEGS-2 ATG mutant (pNSB2110) was created in S2-1.5b (pNSB1869) using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and the primers ...
-
bioRxiv - Cell Biology 2019Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... listed in Table S2) and the site directed deletions and amplification were performed using Pfu turbo DNA polymerase (Agilent, #600250) following manufacturers protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... The mutants were prepared by site directed mutagenesis (see Tables S2 and S3 for details) following the QuikChange site-directed protocol (Agilent). For generation of knockin cell lines the pPLOT blast-mNG-blast plasmid was generated with recoded wild type and mutant profilin genes (synthetized by TWIST Bioscience ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gene sequence was PCR-amplified with primers to introduce T7 promoters on both ends (see Table S2, Oligonucleotides tab) and cloned into pSC-A-amp/kan (Stratagene) vector ...
-
bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed with primers (Table S2) and Brilliant Sybr Green QPCR Reagent Kit (Stratagene, Waldbronn, Germany). The ratio of G ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
-
bioRxiv - Microbiology 2021Quote: ... coli XL1-Blue™ or SURE2™ Supercompetent Cells (Agilent, Germany) using QIAGEN Plasmid Mini and Midi Kit (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... The Escherichia coli strain XL10-Gold (Agilent, Santa Clara, CA, USA) was used as a host for derivatives of plasmid pQE-30 ...
-
bioRxiv - Biochemistry 2020Quote: ... coli XL-1 blue cells (Stratagene California, San Diego, CA, USA). For the cloning and ligation of the amplified gene ...
-
bioRxiv - Biophysics 2021Quote: ACT was expressed in Escherichia coli XL-1 blue cells (Stratagene) transformed with pT7CACT1 plasmid ...
-
bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
-
bioRxiv - Biochemistry 2022Quote: ... DNA manipulations were carried out in Escherichia coli XL2 cells (Agilent). The proteins were expressed in E ...
-
bioRxiv - Biophysics 2019Quote: ... coli BL21-CodonPlus(DE3)-RIPL (Agilent Technologies, Santa Clara, CA, USA) cells were transformed with the resultant plasmids.
-
bioRxiv - Biochemistry 2022Quote: ... Expression was performed in BL21(DE3)- RIPL Escherichia coli cells (Agilent) in LB at room temperature and induced through the addition of 0.2 mM IPTG for ~24 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... All constructs were transformed into Escherichia coli XL10-GOLD cells (Agilent), pEG103 construct transformants were selected using kanamycin (50 μg/mL in solid and 100 μg/mL in liquid medium ...
-
bioRxiv - Biophysics 2023Quote: ACT was expressed in Escherichia coli XL-1 blue cells (Stratagene) transformed with pT7CACT1 plasmid ...
-
bioRxiv - Biochemistry 2023Quote: ... coli BL21-Gold(DE3) competent cells (Agilent, Santa Clara, CA, USA). The expressions of TkSAHH and SfSAHH were performed in E ...
-
bioRxiv - Immunology 2023Quote: ... coli strain BL21 (DE3) pLysS (Agilent Technologies, Santa Clara, CA, USA). The inclusion body containing the κCL was dissolved in 6M guanidine HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... His6-SUMO-ZF5.3 was transformed into Escherichia coli BL21DE3 Gold (Agilent) and selected on a kanamycin Luria Broth (LB ...
-
bioRxiv - Immunology 2021Quote: ... followed by EnVision FLEX DAB+ Chromogen in Substrate buffer (Agilent; anti-SARS-CoV-2, -CD8, -CD45R, -Iba1) for 10 min at RT or the DAB-Map-Kit (Ventana ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Peak areas were integrated with MSD ChemStation software version E.02.01.1177 (Agilent Technologies). Peaks were identified by comparing their ion spectrum to the NIST library (NIST v2.2 ...
-
bioRxiv - Plant Biology 2021Quote: ... Data processing was carried out by MSD Chemstation (E.02.02.1431, Agilent Technologies Inc.). Only VOC peaks with signal- to-noise ratios greater than 10:1 were considered for data quantification ...
-
bioRxiv - Immunology 2020Quote: ... and backbone plasmid pNL4-3.Luc.R-E-(NIH AIDS Reagent) using GeneJammer (Agilent) in 10% FBS/DMEM enriched with 1% Pennicilin/Streptomycin (D10 medium) ...
-
bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used were as follows: E-cadherin antibody (M361201-2, Dako), anti-Filaggrin (sc-66192 ...
-
bioRxiv - Cell Biology 2023Quote: ... 15,000 cells/well were seeded in a 96-well E-plate (Agilent, 5232368001)47 containing gold microelectrodes fused to the bottom surface of the well plate ...
-
bioRxiv - Biochemistry 2024Quote: ... All GC-MS parameters were controlled by ChemStation (G1701EA E.02.02.1431; Agilent Technologies), and data acquisition was completed within 24 h of derivatization ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA was sonicated using COVARIS S2 and peak fragment distribution between 100-500bp was verified on an Agilent Bioanalyzer (Agilent Technologies) using the DNA1000 chip ...
-
bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
-
bioRxiv - Immunology 2021Quote: ... Biotinylated tetrameric spike (1ug) and RBD (0.5ug) were fluorochrome linked for flow cytometry by incubating with streptavidin conjugated APC (Prozyme) and PE (Prozyme ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were expressed using Escherichia coli BL21 gold strain (Agilent Technology Inc.) cultured in Terrific Broth (TB ...
-
bioRxiv - Microbiology 2020Quote: ... coli BL21 CodonPlus (DE3)-RIPL was purchased from Stratagene (La Jolla, CA). The pET-51b vector was obtained from Novagen (San Diego ...