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Citations for Agilent :
551 - 600 of 1327 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 4µm sections were stained with H+E and by IHC for p53 (DAKO IS616), Pax8 (Abcam ab181054 ...
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bioRxiv - Neuroscience 2021Quote: ... and cover-slipped (Catalog #12-544-E) using fluorescence mounting medium (Dako, Cat. #S3023).
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bioRxiv - Molecular Biology 2022Quote: ... The resulting chromatograms were processed using the software MSD ChemStation E.01.00.237 from Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Mahlavu and Huh7 cells were plated into E-96 plates (Agilent, cat. no:5232368001), 1500-2500 cells/wells ...
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bioRxiv - Cancer Biology 2023Quote: Cells were seeded (8000 cells/well) in a 96-well RTCA E-plate (Agilent) in 4 replicates per condition ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene). Melanoma cells were transfected with combinations of reporter constructs ...
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bioRxiv - Immunology 2024Quote: 15,000 B16-OVA cells were seeded onto the E-Plate VIEW 96 (Agilent 300601020) and incubated for a minimum of 5 hours ...
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bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Bioengineering 2022Quote: ... The Escherichia coli strains DH5α and BL21-Gold (DE3) were purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains from glycerol were further analyzed through HPLC (1100 Series HPLC; Agilent) connected with MS (LC/MSD VL ...
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bioRxiv - Biophysics 2022Quote: Escherichia coli BL21-CodonPlus(DE3)-RIPL cells (Agilent Technologies, Santa Clara, CA, USA) were transformed with either pET15-MinD ...
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bioRxiv - Biophysics 2022Quote: ... His6-SUMO-TTL was expressed in Escherichia coli (BL21-DE3 competent cells, Agilent) overnight at 18 °C in LB broth (induced by 0.5 mM IPTG) ...
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bioRxiv - Biochemistry 2022Quote: ... Each construct was transformed into Escherichia coli BL21(DE3) Codon Plus Cells (Agilent). Select colonies were inoculated in 50 ml of Luria Broth (LB ...
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bioRxiv - Biochemistry 2019Quote: ... All constructs were overexpressed in Escherichia coli BL21-gold (DE3) cells (Agilent Technologies) using ZYM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Genomics 2019Quote: ... The plasmid was transformed and expanded in XL10-Gold Escherichia coli strain (Stratagene). The cDNA of the plasmid was sent for Sanger sequencing to validate the gene sequence.
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bioRxiv - Plant Biology 2019Quote: ... and transformed into XL1-BLUE Escherichia coli competent cells (Stratagene, Santa Clara, USA). Three hundred and thirty-six clones (48 from each library ...
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bioRxiv - Biochemistry 2021Quote: ... A freshly transformed colony of Escherichia coli BL21-CodonPLus (DE3)-RIPL cells (Agilent) was inoculated into 100 ml of Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transformed into Eschericia coli BL21-CodonPlus (DE3)-RIL cells (Agilent Technologies) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells from Agilent and purified as previously described (Haizel et al. ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21-ArcticExpress (DE3)-RIL competent cells (Agilent Technologies, Santa Clara, CA, USA) in Terrific Broth modified medium (Melford ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Immunology 2023Quote: ... codon-optimized for Escherichia coli and synthesized as an oligomer pool (Agilent Inc). Each peptide contained an alanine/glycine N-terminus linker (AAVVGGV ...
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bioRxiv - Molecular Biology 2023Quote: ... coli cells (NEB 5-alpha: C2987, Agilent through UCB MacroLab XL1-Blue: 200249) according to the manufacturer’s directions ...
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bioRxiv - Biophysics 2023Quote: ... and eIF4E were expressed and purified from Escherichia coli BL21(DE3) RIPL (Agilent) following established procedures30,35,40,41 ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
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bioRxiv - Microbiology 2020Quote: ... Gene-level intensities were scaled based on the intensity values of ten different in vitro-synthesized transcripts contained in the One Color RNA Spike-In kit (Agilent Technologies) in order to account for technical variation associated with sample processing ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Plant Biology 2020Quote: ... Mutant F161A was made using the Quick-Change II-E site-directed mutagenesis kit (Stratagene) and a pair of complementary primers (Supplementary Table S1) ...
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bioRxiv - Developmental Biology 2021Quote: ... and biotinylated anti-rabbit IgG antibody which is produced in goats (E 0432-1 Dako Cyt ...
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bioRxiv - Biophysics 2021Quote: ... The MutL (R-E) mutation was generated using the QuikChange site-directed mutagenesis kit (Stratagene). Two serine residues separated the his6 and srt ...
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bioRxiv - Cell Biology 2022Quote: ... The E-plate was engaged into the xCELLigence Real Time Cell Analysis (RTCA) Instrument (Agilent) and background measurement of the wells plus growth media was recorded before adding cells in 150 μL of media for each SLC condition (5 x 103 cells/well) ...
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bioRxiv - Systems Biology 2023Quote: ... HBMECs were grown to confluency in xCELLigence 96 PET E-plates (Agilent Technologies Cat# 300600900). On the day of the assay ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Cell Biology 2021Quote: The 3a ORF from SARS CoV-1 (Tor2 strain) was originally obtained from the Institute for Genomic Research and subcloned into pBluescript (Stratagene) prior to transferring to pCAGGS-MCS [17] after PCR amplification with KpnI and XhoI restriction sites ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding the single-mutation and the combination of mutations found in SARS-CoV-2 variants were generated by Quikchange II XL site-directed mutagenesis kit (Agilent). Recombinant Indiana vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... ORF6 mutations were introduced into the pLVX-StrepII-SARS-CoV-2-ORF6-IRES-Puro and pLVX-EF1α-SARS-CoV-2-ORF6-2xStrep-IRES-Puro plasmids using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions using SDM primers listed below ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...
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bioRxiv - Genomics 2021Quote: ... the initial concentrations of purified SARS-CoV-2 and the universal human reference RNA (UHRR, Agilent Technologies, product number 740000) were determined by ddPCR as described above ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Microbiology 2023Quote: ... were prepared and stained with hematoxylin eosin (HE) for histological examination or subjected to immunohistological staining to detect SARS-CoV-2 antigen (performed in an autostainer; Agilent), using the horseradish peroxidase (HRP ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 NTD-RBDL and NTDL-RBD Nucleocapsid constructs were expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Pathology 2024Quote: ... the above IHC protocol was modified such that the anti-SARS-CoV-2 S primary antibody was at 1:1000 dilution (1 µg/mL) in background-reducing antibody diluent (Dako, S302283-2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were propagated in Escherichia coli XL1-Blue cells (Agilent Technologies, Santa Clara, CA), after growth in Lysogeny broth (LB ...
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bioRxiv - Cell Biology 2019Quote: ... The fusion protein was expressed in BL21 Codon Plus (DE3) RIPL Escherichia coli (Agilent) using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen) ...
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bioRxiv - Systems Biology 2021Quote: ... For plasmid propagation chemically competent Escherichia coli XL1-Blue (Agilent Technologies, Santa Clara, CA) cells were used which were grown in lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transformed into Escherichia coli strain BL21-Codon-plus(DE3)-RIL (Stratagene). Bacteria was grown in LB broth in a shaker at 37°C until reaching the log phase (A600nm between 0.4 and 0.5) ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21-Codon-Plus (DE3) RIPL was purchased from Stratagene (La Jolla, CA, USA) and used for protein overexpression ...