Labshake search
Citations for Agilent :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Blocking was performed in 0.03% H2O2 containing sodium azide for 5 minutes and primary antibodies (Table S1) were incubated for 60 minutes before detection (Dako EnVision+ System-HRP labeled Polymer) for 30 minutes and development for 5 minutes ...
-
bioRxiv - Biophysics 2024Quote: ... Liquid chromatography-mass spectrometry (LCMS) was conducted on an Agilent 6550 iFunnel Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to an Agilent 1290 LC system (Agilent) ...
-
bioRxiv - Biophysics 2024Quote: ... coupled to an Agilent 1290 LC system (Agilent). Peptide samples were loaded onto a Sigma-Aldrich Ascentis Peptides ES-C18 column (2.1 mm x 100 mm ...
-
bioRxiv - Biophysics 2024Quote: The Panx1 mutants were engineered with QuickChange II site-directed mutagenesis kit (Stratagene) according to the manufacturer’s specifications ...
-
bioRxiv - Cancer Biology 2024Quote: ... and analyzed via High Sensitivity D5000 ScreenTape (Agilent, cat ...
-
bioRxiv - Cancer Biology 2024Quote: ... and characterized using a Genomic DNA ScreenTape Analysis (Agilent, cat. no’s. 5067-5366 and 5067-5365) on an Agilent 4150 TapeStation system.
-
bioRxiv - Cancer Biology 2024Quote: ... and RNA from the Seraseq Fusion RNA mix v4 (SeraCare, cat. no. 0710-0497) were quality checked using a High Sensitivity RNA ScreenTape (Agilent, cat. no’s. 5067-5579 and 5067-5580) on an Agilent 4150 TapeStation system (Agilent ...
-
bioRxiv - Cancer Biology 2024Quote: The RNA sample (Seraseq® Fusion RNA Mix v4, cat. no. 0710-0497) was quality checked using a High Sensitivity RNA ScreenTape(Agilent, cat. no’s. 5067-5579 and 5067-5580) on an Agilent 4150 TapeStation system (Agilent ...
-
bioRxiv - Cancer Biology 2024Quote: ... and analyzed via High Sensitivity D5000 ScreenTape (Agilent, cat ...
-
bioRxiv - Biophysics 2024Quote: ... Fluorescence spectra were acquired with a Cary Eclipse fluorescence spectrophotometer (Agilent Technologies, USA) with a slit size of 5 nm ...
-
bioRxiv - Biophysics 2024Quote: ... where residues I3 and I5 were replaced with alanine using the Quick-Change Lightning kit following the manufacturer’s instructions (Agilent). Following mutagenesis ...
-
bioRxiv - Biophysics 2024Quote: ... Fluorescence was measured on a Mx3005P qPCR system (Agilent Technologies) using 492 nm as excitation and 610 nm as emission in 85 cycles with a 1 °C temperature increase ...
-
bioRxiv - Biophysics 2024Quote: The recombinant human Cα subunit of cAMP-dependent protein kinase with the Phe to Ala mutation in position 100 (PKA-CF100A) was generated from the human PKA-Cα wild-type using Quik-Change Lightning mutagenesis kit (Agilent genomics). The key resource table lists the PCR primers used to modify the pET-28a expression vector encoding for the wild-type human PKA-Cα gene (PRKACA – uniprot P17612 ...
-
bioRxiv - Biophysics 2024Quote: ... coli BL21 (DE3) pLyss cells (Agilent) were cultured overnight at 30 °C in Luria-Bertani (LB ...
-
bioRxiv - Cell Biology 2024Quote: ... 4 technical replicates for each time point were analysed using Microwave Plasma - Atomic Emission Spectrometry (MP-AES 4210, Agilent) as reported previously24.
-
bioRxiv - Cell Biology 2024Quote: ... and an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). All samples had a 260:280 nm ratio between 1.9 and 2.1 and RNA integrity number ≥ 9.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
-
bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
-
bioRxiv - Cell Biology 2024Quote: ... an MluI restriction site was introduced immediately upstream of the stop codon and XhoI site in pBluescript plasmids bearing full-length Slik and SlikKD coding sequences (PA isoform) as EcoRI-XhoI fragments (Hipfner and Cohen, 2003) by QuickChange mutagenesis (Agilent Technologies). For pUAST/SlikCCD-GFP ...
-
bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
-
bioRxiv - Cell Biology 2024Quote: ... was diluted 1:100 (Antibody Diluent, S0809 Dako Agilent) and cells were incubated for 2 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... was diluted 1:100 (Antibody Diluent, S0809 Dako Agilent) and cells were incubated for 2 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
-
bioRxiv - Cell Biology 2024Quote: ... The staining was performed with the Dako LSAB2 System-HRP kit (Dako, Glostrup, Denmark). Subsequently ...
-
bioRxiv - Cell Biology 2024Quote: ... and incubated for 1 h at 37 °C with blocking buffer (Agilent Technologies, Waldbronn, Germany). Afterwards ...
-
bioRxiv - Biophysics 2024Quote: ... Solution size exclusion chromatography coupled with multi-angle laser light scattering/quasi-elastic light scattering (SEC-MALS/QELS) experiments were conducted using Agilent Technologies 1200 LC HPLS system (Agilent Technologies, Santa Clara, CA) equipped with a Wyatt Dawn® Heleos™II 18-angle MALS light scattering detectors ...
-
bioRxiv - Biophysics 2024Quote: ... the constructs were transformed into BL21-CodonPlus (DE3)-RP competent cells (Agilent). For transformation and precultures ...
-
bioRxiv - Biophysics 2024Quote: Accurate masses of unlabeled and labeled proteins were determined via LC-MS with an Agilent PL1912- 1300 reverse phase HPLC column (Agilent, Santa Clara, CA, USA) connected to an AB Sciex 5600 QTOF mass spectrometer (AB Sciex ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA genomic libraries were prepared from fresh tissue (tumor and nontumor) and FFPE tumor tissue DNA prior to exome capture (SureSelect XT Human All Exon v5, Agilent). Libraries were sequenced on a HiSeq2000 instrument (Illumina ...
-
bioRxiv - Cancer Biology 2024Quote: ... antigen retrieval was performed using target retrieval solution pH 6.0 or pH 9.0 according to antibody protocol recommendations (Agilent, Santa Clara, CA, USA). The samples were scanned (panoramic slide digital scanner ...
-
bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and the OCR was calculated using Wave (version 2.6, Agilent). OCR was normalized to cell number with the CyQUANT NF Cell Proliferaiton Assay (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... and incubated with the conjugated antibody for one hour before being subjected to flow cytometry analysis on an ACEA NovoCyte Quanteon analyzer (Agilent, Santa Clara, CA). For xenograft TME cellular profiling and in vivo cancer cell HLA expression analysis ...
-
bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
-
bioRxiv - Cancer Biology 2024Quote: ... lipids were selectively isolated on a Bravo automated liquid handler platform (Agilent) operated by a VWorks automation protocol as described (5994-5065EN).
-
bioRxiv - Cancer Biology 2024Quote: ... cells were washed and then incubated in XF DMEM medium (pH 7.4, Agilent) with 1 mM pyruvate ...
-
bioRxiv - Cancer Biology 2024Quote: ... sgRNA was amplified from 50 mg gDNA using Herculase II Fusion DNA Polymerase (Agilent Technologies), column purified using Select-a-Size DNA Clean & Concentrator kit (Zymo Research) ...
-
bioRxiv - Cancer Biology 2024Quote: ... analyzed by Agilent TapeStation ...
-
bioRxiv - Cancer Biology 2024Quote: ... The assay was conducted on an XF96 Extracellular Flux Analyzer (Agilent), and the OCR was calculated using Wave (version 2.6 ...
-
bioRxiv - Cancer Biology 2024Quote: ... enrichment of phosphorylated peptides was performed on the Bravo automated liquid handling platform using AssayMAP Fe(III)-NTA cartridges (Agilent). In brief ...
-
bioRxiv - Cancer Biology 2024Quote: Sample clean-up and digest was performed using the SP3 method on an automated Bravo liquid handling platform (Agilent Technologies, CA, US) as previously described75,76 with minor modifications ...
-
bioRxiv - Cell Biology 2024Quote: ... 2 million sperm per well was placed into concanavalin A (0.05 mg/mL) coated 96-well plate for Seahorse XFe96 (Agilent). Mitochondrial activity (OXPHOS ...
-
bioRxiv - Cell Biology 2024Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
-
bioRxiv - Cell Biology 2024Quote: ... the coverslips were mounted with fluorescence mounting medium (S3023; Dako). Images were acquired using an inverted fluorescence phase contrast microscope (Keyence BZ-X810 ...
-
bioRxiv - Cell Biology 2024Quote: ... the sections were incubated with the primary antibodies (as mentioned above) diluted in Antibody Diluent (Agilent Dako, S0809) overnight at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... All subsequent steps were performed through an Agilent Bravo liquid handling robot (Agilent Technologies, USA). 5mM TCEP and 20mM CAA were added and incubated for 30 minutes in the dark to ensure reduction and alkylation of proteins ...
-
bioRxiv - Cancer Biology 2024Quote: ... samples were depleted semi-automatically with an Agilent Multiple Affinity Removal Column Human 14 column (MARS Cat# 5188-6557, Agilent Technologies, USA) on a microflow HPLC system using MARS buffers A and B in a gradient as suggested by the manual ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nanodrop quantified RNA was checked by Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Samples with RNA integrity number > 10 were used for library preparation (Illumina Stranded mRNA Prep kit ...
-
bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and analyzed with NovoCyte Flow Cytometer (Agilent). Median fluorescence intensities (MFI ...
-
bioRxiv - Cancer Biology 2024Quote: ... fragments using Herculase II Fusion DNA Polymerase (Agilent Technologies; cat. 600677) and primers with 30 cycles and annealing at 63°C ...